各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。
这对CONFOCAL去做多重染色时是必不可少的参考资料。
全部文件请到这里下载！Excitation and Emission Wavelengths of FluorophoresTavi\'s \"FluoroTable\": Common fluorophores(from Zeiss Corporation website)Fluorophore Absorption E mission Other info1,5 IAEDANS 3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine(5-TAMRA)5425685-FAM (5-Carboxyfluorescein)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)578567604 5915-TAMRA(5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine 6G518543 6-CR 6G518543 6-JOE520548 7-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471, 474ABQ344445Acid Fuchsin540630ACMA(9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466Photoprotein AFPs - AutoFluorescent Protein -(Quantum Biotechnologies) see sgGFP,sgBFPAlexa Fluor 350346342442 441Alexa Fluor 430431540 Alexa Fluor 488495, 492519, 520 Alexa Fluor 532531, 532553, 554 Alexa Fluor 546556, 557572, 573 Alexa Fluor 568577, 578603 Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650 Alexa Fluor 647647666 Alexa Fluor 660668698 Alexa Fluor 680679702Alizarin Complexon530-560,580580, 624-645Alizarin Red530-560580 Allophycocyanin (APC)630, 645655, 660 AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345347425 444AMCA-X353442 Aminoactinomycin D555655Aminocoumarin346350442 445Aminomethylcoumarin (AMCA)345347425 444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+ APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480 Atabrine436490 ATTO-TAG CBQCA465560 ATTO-TAG FQ486591 Auramine460550 Aurophosphine G450580 Aurophosphine450-490515BAO 9(Bisaminophenyloxadiazole)365395 BCECF (high pH)492, 503520,528 BCECF (low pH)482520 Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H) Blue Fluorescent Protein 381, 382,383445, 447,448blue shifted GFP (Y66H)Blue FluorescentProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+ Blancophor FFG390470Blancophor SV370435BOBO-1462481BOBO-3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodipy Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO-1462481BO-PRO-3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+ BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein 494 517 Calcein Blue 373440Calcium Crimson 588, 589 611, 615 Calcium Green501, 506 531 Calcium Green-1 Ca2+ Dye 506 531 Ca2+ Dye Calcium Green-2 Ca2+ 506/503 536 Ca2+ Calcium Green-5N Ca2+ 506 532 Ca2+ Calcium Green-C18 Ca2+ 509 530 Ca2+ Calcium Orange549575 576Calcofluor White385, 395, 405437, 440, 445 Carboxy-X-rhodamine (5-ROX) 576 601 Cascade Blue377 398 399420 423Cascade Yellow 399 400550 552Catecholamine 410 470 CCF2 (GeneBlazer) CFDA494520CFP - Cyan Fluorescent Protein430, 433, 436, (453) 474, 475, 476,(501)Cyan FluorescentProteinCFP/YFP FRET Chlorophyll480650Chromomycin A 436-460 470 Chromomycin A445 575CL-NERF (Ratio Dye, pH) 504/514 540 Ratio Dye, pH CMFDA494 520 Coelenterazine Ca2+Dye, bioluminescence(429)465Ca2+Dye,bioluminescence, native moleculeCoelenterazine cp (Ca2+ Dye,) (430) 442 Ca2+Dye,bioluminescenceCoelenterazine f (437) 473 Ca2+Dye,bioluminescenceCoelenterazine fcp 452 Ca2+Dye,bioluminescenceCoelenterazine h (437) 464 Ca2+Dye,bioluminescenceCoelenterazine hcp (433) 444 Ca2+Dye,bioluminescenceCoelenterazine ip 441 Ca2+Dye,bioluminescenceCoelenterazine n (431) 467 Ca2+Dye,bioluminescenceCoelenterazine O 460 575 Coumarin Phalloidin 387 470 C-phycocyanineCPM Methylcoumarin 384 469 Methylcoumarin CTC400-450602CTC FormazanCy2489506 8554568581598Cy3514552554566 5708649666675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Cell ProliferationAssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574 DCFDA504529 DCFH (DichlorodihydrofluoresceinDiacetate)505535DDAO463607 DHR (Dihydorhodamine 123)505534 Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488498605 713DiA (4-Di-16-ASP)456591Dichlorodihydrofluorescein Diacetate(DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DiIC18(5))644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3))549, 551565Dinitrophenol349DiO (DiOC18(3))484, 487501, 502DiR748780Lipophilic TracerDiR (DiIC18(7))750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent protein DTAF494520DY-630-NHS621660Hemicyanelabel for proteins andDNADY-635-NHS634664Hemicyanelabel for proteins andDNAEBFP383447Enhanced BlueFluorescent Protein ECFP436474Enhanced CyanFluorescent Protein EGFP488, 498507, 516Enhanced GreenFluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrysin430540EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhanced YellowFluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612520, 527Fluo-3480 506,506Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red(high pH)572657Fura RedFluo-3Fura-2, high calcium335505Excitation ratio dye Fura-2, low calcium363512Excitation ratio dye Fura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516 GFP wild type, non-UV excitation475509 (wtGFP)GFP wild type, UV excitation (wtGFP)395509 GFPuv385508 Gloxalic Acid405460 Granular Blue355425 Haematoporphyrin530-560580 Hoechst 33258345487Hoechst 33342347。