各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。
这对CONFOCAL去做多重染色时是必不可少的参考资料。
全部文件Excitation and Emission Wavelengths of FluorophoresTavi\'s \"FluoroTable\": Common fluorophores(from )Fluorophore Absorption Emission Other info1,5 IAEDANS 3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluorescein)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)578567604 5915-TAMRA (5-Carboxytetramethylrhodamine)548542552 5686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydroxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471, 474ABQ344445Acid Fuchsin540630ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466Photoprotein AFPs -AutoFluorescent Protein -(QuantumBiotechnologies) see sgGFP, sgBFPAlexa Fluor 350346342442 441Alexa Fluor 430431540 Alexa Fluor 488495, 492519, 520 Alexa Fluor 532531, 532553, 554 Alexa Fluor 546556, 557572, 573 Alexa Fluor 568577, 578603 Alexa Fluor 594590, 594617, 618 Alexa Fluor 633632650 Alexa Fluor 647647666 Alexa Fluor 660668698 Alexa Fluor 680679702Alizarin Complexon530-560,580580, 624-645Alizarin Red530-560580 Allophycocyanin (APC)630, 645655, 660 AMC, AMCA-S345445AMCA (Aminomethylcoumarin)345347425 444AMCA-X353442 Aminoactinomycin D555655Aminocoumarin346350442 445Aminomethylcoumarin (AMCA)345347425 444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+ APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG CBQCA465560ATTO-TAG FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H) Blue Fluorescent Protein 381, 382,383445, 447,448blue shifted GFP (Y66H)Blue Fluorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+ Blancophor FFG390470Blancophor SV370435BOBO-1462481BOBO-3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodipy Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO-1462481BO-PRO-3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+ BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+ Calcein494517Calcein Blue373440Calcium Crimson588, 589611, 615Calcium Green501, 506531Calcium Green-1 Ca2+ Dye 506531Ca2+ DyeCalcium Green -2 Ca2+ 506/503 536 Ca2+Calcium Green -5N Ca2+ 506 532 Ca2+ Calcium Green -C18 Ca2+ 509 530 Ca2+ Calcium Orange 549575 576 Calcofluor White385, 395, 405437, 440, 445Carboxy -X -rhodamine (5-ROX) 576 601 Cascade Blue 377398 399420 423Cascade Yellow 399 400 550 552Catecholamine 410 470 CCF2 (GeneBlazer) CFDA 494 520 CFP - Cyan Fluorescent Protein 430, 433, 436, (453) 474, 475, 476, (501)Cyan Fluorescent ProteinCFP/YFP FRET Chlorophyll 480 650 Chromomycin A 436-460 470 Chromomycin A 445 575 CL -NERF (Ratio Dye, pH) 504/514 540 Ratio Dye, pH CMFDA 494 520 Coelenterazine Ca2+ Dye, bioluminescence (429) 465 Ca2+ Dye,bioluminescence, native moleculeCoelenterazine cp (Ca2+ Dye,) (430) 442 Ca2+ Dye,bioluminescenceCoelenterazine f (437) 473 Ca2+ Dye,bioluminescenceCoelenterazine fcp 452 Ca2+ Dye,bioluminescenceCoelenterazine h (437) 464 Ca2+ Dye,bioluminescenceCoelenterazine hcp (433) 444 Ca2+ Dye,bioluminescenceCoelenterazine ip 441 Ca2+ Dye,bioluminescenceCoelenterazine n (431) 467 Ca2+ Dye,bioluminescenceCoelenterazine O 460 575 Coumarin Phalloidin 387 470 C -phycocyanineCPM Methylcoumarin384469Methylcoumarin CTC400-450602CTC FormazanCy2489506Cy3.1 8554568Cy3.5581598Cy3514552554566 570Cy5.1 8649666Cy5.5675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Cell Proliferation Assay Dabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH (Dichlorodihydrofluorescein Diacetate)505535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488498605 713DiA (4-Di-16-ASP)456591 Dichlorodihydrofluorescein Diacetate (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic Tracer DiD (DiIC18(5))644665DIDS341415 Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3))549, 551565Dinitrophenol349DiO (DiOC18(3))484, 487501, 502DiR748780Lipophilic TracerDiR (DiIC18(7))750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent protein DTAF494520DY-630-NHS621660Hemicyanelabel for proteins andDNADY-635-NHS634664Hemicyanelabel for proteins andDNAEBFP383447Enhanced BlueFluorescent Protein ECFP436474Enhanced CyanFluorescent Protein EGFP488, 498507, 516Enhanced GreenFluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrysin430540EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhanced YellowFluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612520, 527Fluo-3480 506,506Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red(high pH)572657Fura RedFluo-3Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 33342347Filter sets installed on the Axiovert 200M:filter cube contains DIC analyzer1) BrightfieldDIC:2) DAPI / BFP: Chroma set 31041, Ex at 380 nm (bandwidth 30 nm), Em at 460 nm(bandwidth 50 nm).3) FITC / GFP:Chroma set 41001, Ex at 480 nm (bandwidth 40 nm), Em at 535 nm (bandwidth 50 nm).4) RHOD:Chroma set 41002c, Ex at 545 nm (bandwidth 30 nm), Em at 620 nm(bandwidth 60 nm).5) Cy5:Chroma set 41024, Ex at 620 nm (bandwidth 60 nm), Em 665 nm and above(long pass filter).Also available upon request:4) GFP:Chroma set 41017, Ex at 470 nm (bandwidth 40 nm), Em 525 nm (band width50 nm).5) CFP:Chroma set 31044v2, Ex at 436 nm (bandwidth 20 nm), Em 480 nm(bandwidth 40 nm).6) YFP: Chroma set 41028, Ex at 500 (bandwidth 20 nm), Em 535 (bandwidth 30 nm).。