SDS-PAGE浓缩胶/堆积胶/上层胶配方表
成分配制不同体积SDS-PAGE浓缩胶所需各成分的体积（毫升）5%胶235681015 蒸馏水 1.4 2.1 3.5 4.1 5.5 6.810.3 30% ACR-Bis（29:1）0.330.50.831 1.3 1.7 2.53 1M Tris Buf.，pH6.80.250.380.630.751 1.25 1.88 10% SDS0.020.030.050.060.080.10.15 10% AP（过硫酸铵）0.020.030.050.060.080.10.15 TEMED0.0020.0030.0050.0060.0080.010.015
SDS-PAGE分离胶配方表
成分配制不同体积SDS-PAGE分离胶所需各成分的体积（毫升）6%胶5101520253050 蒸馏水2468101220 30% ACR-Bis（29:1）12345610 1M Tris Buf.，pH8.8 1.9 3.8 5.77.69.511.419 10% SDS0.050.10.150.20.250.30.5 10% AP（过硫酸铵）0.050.10.150.20.250.30.5 TEMED0.0030.0060.0090.0120.0150.0180.03成分配制不同体积SDS-PAGE分离胶所需各成分的体积（毫升）8%胶5101520253050 蒸馏水 1.7 3.35 6.78.31016.7 30% ACR-Bis（29:1） 1.3 2.74 5.3 6.7813.3 1M Tris Buf.，pH8.8 1.9 3.8 5.77.69.511.419 10% SDS0.050.10.150.20.250.30.5 10% AP（过硫酸铵）0.050.10.150.20.250.30.5 TEMED0.0030.0060.0090.0120.0150.0180.03成分配制不同体积SDS-PAGE分离胶所需各成分的体积（毫升）10%胶5101520253050 蒸馏水 1.3 2.74 5.3 6.7813.3 30% ACR-Bis（29:1） 1.7 3.35 6.78.31016.7 1M Tris Buf.，pH8.8 1.9 3.8 5.77.69.511.419 10% SDS0.050.10.150.20.250.30.5 10% AP（过硫酸铵）0.050.10.150.20.250.30.5 TEMED0.0030.0060.0090.0120.0150.0180.03成分配制不同体积SDS-PAGE分离胶所需各成分的体积（毫升）12%胶5101520253050 蒸馏水12345610 30% ACR-Bis（29:1）2468101220 1M Tris Buf.，pH8.8 1.9 3.8 5.77.69.511.419 10% SDS0.050.10.150.20.250.30.5 10% AP（过硫酸铵）0.050.10.150.20.250.30.5 TEMED0.0030.0060.0090.0120.0150.0180.03成分配制不同体积SDS-PAGE分离胶所需各成分的体积（毫升）15%胶5101520253050 蒸馏水0.51 1.52 2.535 30% ACR-Bis（29:1） 2.557.51012.51525 1M Tris Buf.，pH8.8 1.9 3.8 5.77.69.511.419 10% SDS0.050.10.150.20.250.30.5 10% AP（过硫酸铵）0.050.10.150.20.250.30.5 TEMED0.0030.0060.0090.0120.0150.0180.03
备注：如配制非变性胶，参考上述配方不加10% SDS即可。