《生物信息学》大作业 参考答案
芍药 ACS 基因的生物信息学分析 ห้องสมุดไป่ตู้
姓名： 班级： 学号： 2016 年 4 月 11 日
一、芍药 ACS 基因序列及其编码的蛋白的功能 乙烯是存在于植物体内的唯一的一种气态植物激素，调控植物花、果和叶片的衰老进程。乙烯的合成 主要在转录水平上受到 ACS（ACC synthase，ACC 合酶）和 ACC 氧化酶的调控，ACS 将 SAM（S-adenosyl
a b s t r a c t
1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) is a rate-limiting enzyme in the biosynthesis of ethylene which regulates many aspects of the plant development and responses to biotic and abiotic stresses. In this study, a full-length cDNA of ACC synthase, OnACS2, was cloned from the senescing flower of Oncidium Gower Ramsey by RACE. The full-length cDNA of OnACS2 (GenBank accession no. JQ822087) was 1557 bp in length with an open reading frame (ORF) of 1308 bp encoding for a protein of 435 amino acid residues. The predicted OnACS2 protein had a molecular mass of 49.1 kDa with pI value of 7.51. Phylogenetic analysis indicated its evolutionary relationships with corresponding orthologous sequences in orchids, Hosta ventricosa and monocots. Real-time PCR assay demonstrated that OnACS2 was constitutively expressed in all tested organs with the highest transcript level in the gynandria. Differential expression pattern of OnACS2 gene correlated to the ethylene production and the subsequent occurrence of senescent symptoms in flower suggested that OnACS2 probably played an important role in the initiation of flower senescence. © 2015 Elsevier Inc. All rights reserved.
methionine，S-腺苷甲硫氨酸）转化为 ACC，ACC 氧化酶进一步将 ACC 转化为乙烯。 （3 分）
本作业的研究材料为芍药 ACS（ACC synthase）基因，NCBI 序列号为 JX512359，其核苷酸序列及编 码的氨基酸序列如图 1。 （5 分）
acatggggacagcatacgcaataaaagcaataccaaacttcctt taaatctgcttctgctattcgatcatcattgttacattcttctctacaaacctcctctgttttttcttcaaatttctatagtcacataaa ATGGGATTCATGTCCACAGATCAACAAAAGCAATTGCTGTCAAAGATGGCAACAGGCAATGGCCATGGAGAAGACTCTCCTTACTTTGAT M G F M S T D Q Q K Q L L S K M A T G N G H G E D S P Y F D GGTTGGAAGGCATATGACAGCAATCCATTTCATCTTAATAACAATCCTAATGGGGTTATCCAAATGGGACTTGTAGAAAATCTGCTTTCC G W K A Y D S N P F H L N N N P N G V I Q M G L V E N L L S TTTGATGTGATTCAAGAATGGGTTCTGAATAATCCAAAAGCCTCCATTTGCACGCCAGAAGGAATTAATGAATTCAGAGATACTGCTATT F D V I Q E W V L N N P K A S I C T P E G I N E F R D T A I TTTCAGGATTATCATGGGTTTCCAGAGTTCAGAAATGCTATTGCAAAATTTATGGGAAAAGTGGGAGGAGGAAGAGTCACATTCGATCCA F Q D Y H G F P E F R N A I A K F M G K V G G G R V T F D P GACCGCATTGTCATGAGTGGTGGGGCGACTGGAGCTCATGAGATTCTGGCCTTCTGCTTGGCTGACCCTGGCGATGCATTTCTGGTGCCA D R I V M S G G A T G A H E I L A F C L A D P G D A F L V P ACTCCATATTATCCAGGATATGATCGCGATTTGAGATGGCGAACAGGAGCTCAACTGCTTCCCGTTCAATGCGACAGCTCCAACAATTTC T P Y Y P G Y D R D L R W R T G A Q L L P V Q C D S S N N F ATGGTTACCATAAGCGCCCTAGAATTGGCATACCAGAAAGCTCAAGATGCAAACATTAAAGTAAAGGGTTTGATCATAAACAACCCATCA M V T I S A L E L A Y Q K A Q D A N I K V K G L I I N N P S AATCCATTAGGCACTGTCTTAAATGGAGAGACACTAAAAACTATAGTGAACTTCATCAATGAAAAGAACATCCACCTTGTTTGTGATGAG N P L G T V L N G E T L K T I V N F I N E K N I H L V C D E ATTTACGCGGCCACTGTCTTTTGCCAGCCTCGTTTCATTAGCATTGCGGAAATAATAAACGACATGGATGGTGTTAATCGAAATCTCATC I Y A A T V F C Q P R F I S I A E I I N D M D G V N R N L I CACATTGTCTACAGTCTCTCAAAGGACATGGGGTTCCCTGGATTTAGGGTTGGCATTGTGTATTCATACAATGATGCCGTAGTCAGTTGT H I V Y S L S K D M G F P G F R V G I V Y S Y N D A V V S C GCGCGCAAGATGTCTAGCTTTGGGCTAGTTTCAACTCAAACCCAACACCTAATTGCATCAATGCTATCAGATGAACACTTCACCGAGAGA A R K M S S F G L V S T Q T Q H L I A S M L S D E H F T E R TATATTGTGGAGAGTGCAAAGAAATTGGCAGAAAGGCAGAGGCGCTTCACTAGGGGACTTGCTCAAGTAGGCATTAATTTTTTGAAGAGC Y I V E S A K K L A E R Q R R F T R G L A Q V G I N F L K S AATGCAGGTCTCTTCTTATGGATGGATTTAAGCTCGCTGCTCAAGGAGAAGACGGTCGAAGAAGAGCTAACACTTTGGCGAGTGATAATC N A G L F L W M D L S S L L K E K T V E E E L T L W R V I I AATGAAGTTAAACTCAATGTTTCACCTGGTTCATCTTTTCATTGCTCGGAGCCTGGATGGTTTAGGGTTTGCTTTGCTAACATGGATGAT N E V K L N V S P G S S F H C S E P G W F R V C F A N M D D GCCACCATGGAAGTTGCTCTTCGAAGGATTCGAACATTTGCACTTAAGGCCAAGGAAGCAGATGTGCCAAGGAAGAAACAAAGTTGGCAA A T M E V A L R R I R T F A L K A K E A D V P R K K Q S W Q AACAACAACCTTAGATTCAGCTTCAAATCTGGGAAATATGATGATGTCTTGTTGTCTCCTCGTATGATGTCCCCTTGCATGAGGTCCCCT N N N L R F S F K S G K Y D D V L L S P R M M S P C M R S P CATTCCCCCATACCCCAATCACCCCTTGTTCGAGCTTAAttactcggtccatctgtaattaagatcaggaaggttagtgtatgtaattta H S P I P Q S P L V R A * gaccaaattgatgcattctttcctgtggtaatagccaataaaacatggaccatttgatcaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 44 134 224 30 314 60 404 90 494 120 584 150 674 180 764 210 854 240 944 270 1034 300 1124 330 1214 360 1304 390 1394 420 1484 450 1574 480 1664 492 1752