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基础医学实验学生实验守则

Rotundine •结构为四氢巴马汀,镇痛作用比dolantin 弱,无成瘾性。 •阻断脑内多巴胺受体,增加与痛觉有关 的特定脑区脑啡肽原和内啡肽原的 mRNA表达,促进脑啡肽和内啡肽的释 放,产生镇痛作用。
实验材料
1. 实验动物:小鼠(雌性,体重25-30g); 2. 实验器材:数控超级恒温槽,金属罐(热板,hot plate); 3. 实验试剂:2.5g/L dolantin,2.5g/L rotundine sulfate,生
5、实验期间,不得喧哗,保持安静,禁止串岗、饮食。垃圾按类投放。 保持实验室整洁卫生。
6、实验完毕,按要求清理实验台桌,清洁实验室,并将器具等复位。 经指导老师检查后,方可离开实验室。
医学基础综合实验
实验17 热板法镇痛实验
王梦令 Ph. D(ml_wang128@) 浙江大学医学院
实验目的
1. 学习热板法镇痛实验方法; 2. 观察麻醉性镇痛药派替啶(dolantin)与非麻醉性镇痛药
罗通定(rotundine)的镇痛效应。
实验原理
Dolantin • 吗啡的代用品,较吗啡的成瘾性轻。 •作用于中枢神经系统的阿片受体而发挥作用, 镇痛效力约比吗啡弱10倍,持续时间也比吗 啡短。
组别
生理盐水组 哌替啶组 罗通定组
给药后时间(min)
0
15
30
45
பைடு நூலகம்
60
注: * p<0.05,** p<0.01 vs 生理盐水组; # p<0.05,## p<0.01 vs 罗通定组
Pain threshold elevated rate (%)
control
150
rotundine
dolantin
3、在掌握实验操作规程情况下,严格按实验操作规程进行实验。禁止 搬移仪器和作与实验无关的操作。按规定领用、存放实验用品。禁止将实 验室物品带出实验室。丢失或损坏实验用品按规定赔偿。
4、实验期间须严格遵守实验室规章制度和实验操作规程操作,保证实 验室和学生本人自身的安全。凡违反实验室规章制度和实验操作规程,造 成事故和损害,当事人负全责。
rotundine group
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