Bee

taco Total DNA Lysis Buffer:250 μl N/A
N/A
4
200 μl
N/A
PBS:5000 μl
200 μl
N/A
N/A
Canine
Conjunctival PBS:1000 μl swab Whole Blood N/A
Feline
Conjunctival PBS:1000 μl swab Whole Blood N/A
1. Swirl the swab in 1 ml PBS for 30 seconds. taco DNA/RNA 2. Centrifuge with cubee for 1 minute. Extraction Kit 3. Transfer 200 μl of the supernatant for extraction. 1. Swirl the swab in 1 ml PBS for 30 seconds. taco DNA/RNA 2. Centrifuge with cubee for 1 minute. Extraction Kit 3. Transfer 200 μl of the supernatant for extraction. 1. 12000 x g for 1 minutes discard the supernatant. 2. Homogenize sopre with lysis buffer by tacoPrep. taco Total DNA 3. Centrifuge at 12000 x g for 5 minutes to spin down the Extraction Kit debris. 3. Transfer 200 μl of the supernatant for extraction. taco DNA/RNA N/A Extraction Kit 1. Use the sterile artificial insemination (AI) pipette to scrape vaginal mucous several times. taco DNA/RNA 2. Take out the AI pipette and put into centrifuge tube Extraction Kit with 5000 μl PBS. Then mix well. 1. Swirl the swab in 1 ml PBS for 30 seconds. taco DNA/RNA 2. Centrifuge with cubee for 1 minute. Extraction Kit 3. Transfer 200 μl of the supernatant for extraction. taco DNA/RNA N/A Extraction Kit 1. Swirl the swab in 1 ml PBS for 30 seconds. taco DNA/RNA 2. Centrifuge with cubee for 1 minute. Extraction Kit 3. Transfer 200 μl of the supernatant for extraction. taco DNA/RNA N/A Extraction Kit 1. Swirl the swab in 1 ml PBS for 30 seconds. taco DNA/RNA 2. Centrifuge with cubee for 1 minute. Extraction Kit 3. Transfer 200 μl of the supernatant for extraction. taco DNA/RNA N/A Extraction Kit
200 μl
50-200 μl
200 μl
50-200 μl
200 μl
50-200 μl
200 μl
50-200 μl
200 μl 200 μl 200 μl 200 μl 200 μl 200 μl
50-200 μl 50-200 μl 50-200 μl 50-200 μl 50-200 μl 50-200 μl Page 2/5
taco DNA/RNA Extraction Kit
400 μl
50-200 μl
taco DNA/RNA Extraction Kit
200 μl
50-200 μl
taco DNA/RNA Extraction Kit taco DNA/RNA Extraction Kit taco DNA/RNA Extraction Kit taco DNA/RNA Extraction Kit taco DNA/RNA Extraction Kit
GeneReach Biotechnology Corp.
Sample Preparation Protocol (2015/12)
Classify Pretreatment Sample Sample type Buffer Size Semen N/A N/A sample Whole blood Upper respiratory tract swab Lymph node N/A PBS:1000 μl 200 μl 1 swab Beating beads N/A N/A N/A tacoPrep Cycle N/A N/A N/A Procedure 1. Centrifuge with cubee for 10 minute. 2. Transfer 200 μl of the supernatant for extraction. N/A 1. Swirl the swab in 1 ml PBS for 30 seconds. 2. Centrifuge with cubee for 1 minute. 3. Transfer 200 μl of the supernatant for extraction. 1. Homogenize tissue by tacoPrep or disposable grinder. 2. Centrifuge by cubee for 1 minute to spin down the debris. 3. Transfer 100 μl of the supernatant for extraction. 1. Homogenize tissue by tacoPrep or disposable grinder. 2. Centrifuge by cubee for 5 minute to spin down the debris. 3. Transfer 100 μl of the supernatant for extraction. 1. Swirl the swab in 1 ml PBS for 30 seconds. 2. Centrifuge with cubee for 10 seconds. 3. Transfer 200 μl of the supernatant for extraction. 1. Swirl the swab in 1 ml PBS for 30 seconds. 2. Centrifuge with cubee for 10 seconds. 3. Transfer 200 μl of the supernatant for extraction. 1. Swirl the swab in 1 ml PBS for 30 seconds. 2. Centrifuge with cubee for 5 minutes. 3. Transfer 200 μl of the supernatant for extraction. N/A 1. Homogenize tissue by tacoPrep or disposable grinder. 2. Centrifuge by cubee for 1 minute to spin down the debris. 3. Transfer 100 μl of the supernatant for extraction. taco Kit taco DNA/RNA Extraction Kit taco DNA/RNA Extraction Kit taco DNA/RNA Extraction Kit taco DNA/RNA Extraction Kit Supernatant Eluting Input Volume Volume 200 μl 200 μl 200 μl 50-200 μl 50-200 μl 50-200 μl
Sample Preparation Protocol ( 2015/12)
Pretreatment Sample Beating tacoPrep Classify Sample type Procedure Buffer Size beads Cycle taco 1. Homogenize tissue by tacoPrep or disposable grinder. Fish fin 2.5 mm DNA/RNA 2. Vortex for 30 seconds. Centrifuge at 12000 x g for 5 (pectoral 20 mg Steel Beads 1 Lysis minutes to spin down the debris. fins) x6 Buffer:500 μl 3. Transfer 400 μl of the supernatant for extraction. taco 1. Homogenize tissue by tacoPrep or disposable grinder. 2.5 mm DNA/RNA 2. Vortex for 30 seconds. Centrifuge at 12000 x g for 5 Fish gill 20 mg Steel Beads 1 Lysis minutes to spin down the debris. x6 Buffer:500 μl 3. Transfer 400 μl of the supernatant for extraction. Aquaculture 1. Homogenize tissue by tacoPrep or disposable grinder 2.5 mm 2. Centrifuge by cubee for 1 minute to spin down the Fish kidney PBS 500 µl 40 mg Steel Beads 1 debris. x6 3. Transfer 200 μl of the supernatant for extraction. 1. Homogenize tissue by tacoPrep or disposable grinder. 2.5 mm 2. Centrifuge by cubee for 1 minute to spin down the Fish spleen PBS 500 µl 40 mg Steel Beads 1 debris. x6 3. Transfer 200 μl of the supernatant for extraction. Joint (hock) N/A 200 μl N/A N/A Centrifuge with cubee for 5 minutes fluid 1. Homogenize tissue by tacoPrep or disposable grinder. 2.5 mm 2. Centrifuge by cubee for 1 minute to spin down the Liver PBS 500 µl 40 mg Steel Beads 2 debris. x6 3. Transfer 200 μl of the supernatant for extraction. Avian 1. Swirl the swab in 1 ml PBS for 30 seconds. Nasal cavity PBS 500 µl 1 swab N/A N/A 2. Centrifuge with cubee for 1 minute. Swab 3. Transfer 200 μl of the supernatant for extraction. 1. Homogenize tissue by tacoPrep or disposable grinder. 2.5 mm 2. Centrifuge by cubee for 1 minute to spin down the Spleen PBS 500 µl 40 mg Steel Beads 2 debris. x6 3. Transfer 200 μl of the supernatant for extraction. * PBS: Phosphate-Buffered Saline. taco Kit taco DNA/RNA Extraction Kit Supernatant Eluting Input Volume Volume 400 μl 50-200 μl