STAT
binding
STAT
binding
STAT
binding
STAT
binding
STAT
binding
STAT
binding
FNFibronectin III type III domain FNFibronectin
CCCC type
FN Fibronectin
WS-WStype
III with WSXWS
binding
Jak 1
Jak 1
Tyk 2
Jak 1
Jak 2
Jak 1
Jak 1 IRS SOCS
Tyk 2
Jak 3
Jak 1 SOCS SHP STAT
binding
Jak 3
Jak 3 STAT
binding
Jak 3
STAT
binding
STAT
binding
STAT STAT
binding binding
Cloning an organism from somatic cells
Egg cell donor Perform nucleus removal under microscope
Nuclear DNA donor (subject to be cloned)
Grow and select cell for fusion
Fuse anucleated egg cell with somatic cell
Culture fused cells for several cell divisions
Implant embrio in uterus of a third animal
Modular Structure of Cytokine Receptors
IRS-binding
III domain
SHP Jak 2 STATSTAT-binding
binding
SHP
SHP-binding
IRS
III
III domain with cysteines
SOCSSOCS-binding
Tyk 2
JAK/TYK -binding
Ig
Ig - like
Embryonic stem cells
Allergen
PAF, LTC4
Eosinophil
IgE
PGD2, PAF, LTC4, Histamine
Tryptase
FcRI
EMBP
PAR-2 PGD2, PGE2, LTB4
Mast cell
Histamine
Endothelial cells
Vascular permeability, Edema
Ig
FN
CCCC
Ig
FN
CCCC
FN
CCCC
FN
CCCC
FN III FN III FN III FN III FN III FN FN III FN FN III FN FN III FN FN III FN FN III FN
IFNR2 515 aa IL-10R2 325 aa INFR2 332 aa
III
FN 449 aa WS-WS III FN
IL-6R
IL-11R 400 aa
III III
III III
III III
FN
WS-WS
FN
WS-WS
FN
WS-WS
FN
FN III FN
FN III FN III FN III
Ig
FN
CCCC
Ig
FN
CCCC
Ig
FN
CCCC
Ig
FN
CCCC
III FN III FN III
Macrophage M
IL-4 IL-10 INF- IL-17 MIF
FcRI
Haematopoiesis
Basophil
CFU-B Dendritic cell
CFU-DC Macrophage
CMP
CFU-GM
CFU-M Granulocyte CFU-G
HSC Platelets HSC CFU-MK Erythrocyte CFU-E
MPP
HSC
Early NK
NK cell
CLP
Pro-T
Pre-T
T-cell
Pro-B
Pre-B
B-cell
FcRI
Chymase
LTC4
Neuronal cells Smooth muscle cells
Vasodilation
Immune cell interactions in inflammation
Autocrine loop: FGF TGF
Fibroblasts
Antigen, Autoantigen
IL-6
Monocyte
Antibodies
IL-1 TNF IL-18 IL-10 MIF IL-6 IL-8 GM-CSF
B-cell
MHC II
FcRI
MCP-1 IL-1, TNF, IL-8
Th1
Autocrine loop: IL-1 IL-15 GM-CSF TNF MIF
MMPs
Ig
FN
CCCC
Ig
FN
CCCC
III FN III FN III
III
III III
III III
III III
III III
III III
III
FN III
FN
WS-WS
FN
WS-WS
FN
WS-WS
FN
WS-WS
FN
WS-WS
FN
WS-WS
III
III
III
III
III
III
III
Tyk 2 STAT
Potential use for therapeutic cloning
Use for In Vitro fertilization
Freeze in N2 for long-term storage
Mast cells and Eosinophils in ocular allergic response
OSM-R 952 aa gp130 896 aa IL-12 662 aa
LIF-R 1053 aa
FN
CCCC
III III
FN
WS-WS
FNFNR1 530 aa IL-10R1 557 aa INFR1 489 aa IL-22R1 574 aa IL-4R 825 aa IL-13R1 427 aa c 349 aa IL-13R2 380 aa
Sperm donor
Egg fertilization
Culture fertilized cells for several cell divisions
Egg donor
Dissociate cell cluster for subculturing and further utilization