0
0
CGC CATATG GCG
0
0
GGGTTT CATATG AAACCC
0
0
GGAATTC CAT ATG GAATTCC
75
>90
GGGAATTC CATATG GAATTCCC
75
>90
Nhel
GGCTAGC C
0
0
CG GCTAGC CG
10
25
CTA GCTAGC TAG
10
50
切割率%
CGC GGATCC GCG
>90
>90
Bglll
CAGATCT G
0
0
GAAGATCT TC
75
>90
GGA AGATCT TCC
25
>90
BssHII
GGCGCGC C
0
0
AG GCGCGC CT
0
0
TTG GCGCGC CAA
50
>90
1BstEII
GGGT(A/T)ACC C
0
10
BstXI
10
25
TCG CGATCG CGA
0
10
SacI
CGAGCTC G
10
10
Sacll
GCCGCGG C
0
0
TCC CCGCGG GGA
50
>90
Sall
GTCGAC GTCAAAAGGCCATAGCGGCCGC
0
0
GCGTCGAC GTCTTGGCCATAGCGGCCGCG
10
50
G
10
75
ACGC GTCGAC GTCGGCCATAGCGGCCGCG
GAA
Seal
GAGTACT C
10
25
AAA AGTACT TTT
75
75
Smal
CCCGGG
0
10
CCCCGGG G
0
10
CCCCCGGG GG
10
50
TCC CCCGGG GGA
>90
>90
Sp el
GACTAGT C
10
>90
GG ACTAGT CC
10
>90
CGG ACTAGT CCG
0
50
Hale Waihona Puke 1075KpnlGGGTACC C
0
0
GG GGTACC CC
>90
>90
CGG GGTACC CCG
>90
>90
Mlul
GACGCGT C
0
0
CG ACGCGT CG
25
50
Ncol
CCCATGG G
0
0
CATG CCATGG CATG
50
75
Ndel
CCATATG G
0
0
CC CATATG GG
CTAG ACTAGT CTAG
0
50
Sp hl
GGCATGC C
0
0
CAT GCATGC ATG
0
25
ACAT GCATGC ATGT
10
50
Stul
AAGGCCT T
>90
>90
GA AGGCCT TC
>90
>90
AAA AGGCCT TTT
>90
>90
Xbal
CTCTAGA G
0
0
GC TCTAGA GC
AACTGCAGAA CCAATGCATTGG
0
0
AAAACTGCAG CCAATGCATTGG AA
25
50
CTGCAGAA CCAATGCATTGG ATGCAT
25
>90
Clal
CATCGAT G
0
0
GATCGAT C
0
0
CCATCGAT GG
>90
>90
CCC ATCGAT GGG
50
50
EcoRI
75
>90
PstI
GCTGCAG C
0
0
TGCA CTGCAG TGCA
10
10
AACTGCAG AACCAATGCATTGG
>90
>90
AAAA CTGCAG CCAATGCATTGGAA
>90
>90
CTGCAG AACCAATGCATTGGATGCAT
0
0
P vul
CCGATCG G
0
0
ATCGATCG AT
>90
>90
TGC TCTAGA GCA
75
>90
CTAG TCTAGA CTAG
75
>90
Xhol
CCTCGAG G
0
0
CC CTCGAG GG
10
25
CCG CTCGAG CGG
10
75
Xmal
CCCCGGG G
0
0
CCCCCGGG GG
25
75
CCC CCCGGG GGG
50
>90
TCCC CCCGGG GGGA
不同内切酶对识别位点以外最少保护碱基数目的要求
不同内切酶对识别位点以外最少保护碱基数目的要求
酶
切割率%
寡核甘酸序列
2hr
20hr
AccI
GGTCGAC C
0
0
CG GTCGAC CG
0
0
CCG GTCGAC CGG
0
0
Afllll
CACATGT G
0
0
CCACATGT GG
>90
>90
CCC ACATGT GGG
酶
寡核甘酸序列
2hr
20hr
Notl
TTGCGGCCGC AA
0
0
ATTTGCGGCCGC TTTA
10
10
AAATAT GCGGCCGC TATAAA
10
10
ATAAGAAT GCGGCCGC TAAACTAT
25
90
AAGGAAAAAA GCGGCCGC AAAAGGAAAA
25
>90
Nsil
TGC ATGCAT GCA
GGAATTC C
>90
>90
CG GAATTC CG
>90
>90
CCG GAATTC CGG
>90
>90
Haelll
GG GGCC CC
>90
>90
AGC GGCC GCT
>90
>90
TTGC GGCC GCAA
>90
>90
Hi ndlll
CAAGCTT G
0
0
CCAAGCTT GG
0
0
CCC AAGCTT GGG
>90
>90
10
>90
CCA ATGCAT TGGTTCTGCAGTT
>90
>90
P acl
TTAATTAA
0
0
GTTAATTAA C
0
25
CC TTAATTAA GG
0
>90
Pmel
GTTTAAAC
0
0
GGTTTAAAC C
0
25
GGGTTTAAAC CC
0
50
AGCTTT GTTTAAAC GGCGCGCCGG
>90
>90
AscI
GGCGCGCC
>90
>90
AGGCGCGCC T
>90
>90
TT GGCGCGCC AA
>90
>90
Aval
CCCCGGG G
50
>90
CCCCCGGG GG
>90
>90
TCC CCCGGG GGA
>90
>90
BamHI
CGGATCC G
10
25
CG GGATCC CG
>90
>90