Rosetta系列的表达菌株可以提供T7 RNA聚合酶，它能表达PET系列载体上的外源基因。

pGEX系列载体上的外源基因不需要T7 RNA聚合酶，普通的大肠杆菌经IPTG诱导即可表达Tac启动子是一组由Lac和trp启动子人工构建的杂合启动子，受Lac阻遏蛋白的负调节，它的启动能力比Lac和trp都强。

其中Tac 1是由Trp启动子的－35区加上一个合成的46 bp DNA片段(包括Pribnow 盒)和Lac操纵基因构成，Tac 12是由Trp的启动子-35区和Lac 启动子的-10区，加上Lac操纵子中的操纵基因部分和SD序列融合而成蛋白标签：pGEX4T1载体基本信息出品公司: GE别名: pGEX-4T-1, pGEX4T1, pGEX 4T 1质粒类型: 大肠杆菌蛋白表达载体表达水平: 高拷贝启动子: Tac克隆方法: 多克隆位点，限制性内切酶载体大小: 4969 bp5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG载体标签: N-GST载体抗性: Ampicillin 氨苄备注: 复制子是pMB1产品目录号: 27-4580-01稳定性: 瞬时表达 Transient组成型: 诱导表达病毒/非病毒: 非病毒pGEX4T1载体质粒图谱和多克隆位点信息原核生物DNA复制起始点，是DNA链上独特的具有起始DNA复制功能的碱基序列。

大肠杆菌的复制起pGEX4T1载体简介pGEX4T1载体序列LOCUS pGEX-4T-1 4969 bp DNA circular SYN DEFINITION pGEX-4T-1ACCESSIONKEYWORDSSOURCEORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI/COMMENT VNTAUTHORNAME||FEATURES Location/Qualifierssource 1..4969/organism="pGEX-4T-1"/mol_type="other DNA"promoter 184..212/label="tac_promoter"misc_feature 224..246/label="M13_pUC_rev_primer"gene 258..977/label="GST (variant)"/gene="GST (variant)"CDS 258..977/label="ORF frame 3"/label="pGEX_3_primer"promoter 1307..1335/label="AmpR_promoter"gene 1377..2237/label="Ampicillin"/gene="Ampicillin"CDS 1377..2237/label="ORF frame 3"rep_origin 2392..3011/label="pBR322_origin"misc_feature 3309..4400/label="lacI"CDS 3441..4400/label="ORF frame 3"promoter 4449..4478/label="lac_promoter"misc_feature 4492..4514/label="M13_pUC_rev_primer" promoter 4513..4531/label="M13_reverse_primer" CDS 4523..81/label="ORF frame 2"misc_feature 4540..4695/label="lacZ_a"/label="M13_forward20_primer"misc_feature complement(4552..4574)/label="M13_pUC_fwd_primer"pGEX4T2载体基本信息出品公司: GE别名: pGEX-4T-2, pGEX4T2, pGEX 4T 2 质粒类型: 大肠杆菌蛋白表达载体表达水平: 高拷贝启动子: Tac克隆方法: 多克隆位点，限制性内切酶载体大小: 4970 bp5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG 3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG 载体标签: N-GST载体抗性: Ampicillin 氨苄备注: 复制子是pMB1产品目录号: 27-4581-01稳定性: 瞬时表达 Transient组成型: 诱导表达病毒/非病毒: 非病毒pGEX4T2载体质粒图谱和多克隆位点信息pGEX4T2载体简介pGEX4T2载体序列LOCUS pGEX-4T-2 4970 bp DNA circular SYN DEFINITION pGEX-4T-2ACCESSIONKEYWORDSSOURCEORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI/COMMENT VNTAUTHORNAME||FEATURES Location/Qualifiers/organism="pGEX-4T-2"/mol_type="other DNA"promoter 184..212/label="tac_promoter"misc_feature 224..246/label="M13_pUC_rev_primer" gene 258..978/label="GST (variant)"/gene="GST (variant)"CDS 258..974/label="ORF frame 3"misc_feature complement(1020..1042)/label="pGEX_3_primer"promoter 1308..1336/label="AmpR_promoter"gene 1378..2238/label="Ampicillin"/gene="Ampicillin"CDS 1378..2238/label="ORF frame 1"rep_origin 2393..3012/label="pBR322_origin"misc_feature 3310..4401/label="lacI"/label="ORF frame 1"promoter 4450..4479/label="lac_promoter"misc_feature 4493..4515/label="M13_pUC_rev_primer"promoter 4514..4532/label="M13_reverse_primer"CDS 4524..81/label="ORF frame 3"misc_feature 4541..4696/label="lacZ_a"promoter complement(4544..4560)/label="M13_forward20_primer" misc_feature complement(4553..4575)/label="M13_pUC_fwd_primer" pGEX4T3载体质粒图谱和多克隆位点信息pGEX4T3载体简介pGEX4T3载体序列LOCUS pGEX-4T-3 4968 bp DNA circular SYN DEFINITION pGEX-4T-3ACCESSIONKEYWORDSSOURCEORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI/COMMENT VNTAUTHORNAME||FEATURES Location/Qualifierssource 1..4968/organism="pGEX-4T-3"/mol_type="other DNA"promoter 184..212/label="tac_promoter"misc_feature 224..246/label="M13_pUC_rev_primer" gene 258..976/label="GST (variant)"/gene="GST (variant)"CDS 258..980/label="ORF frame 3"misc_feature complement(1018..1040)/label="pGEX_3_primer"promoter 1306..1334/label="AmpR_promoter"gene 1376..2236/label="Ampicillin"/gene="Ampicillin"CDS 1376..2236/label="ORF frame 2"rep_origin 2391..3010/label="pBR322_origin"misc_feature 3308..4399/label="lacI"CDS 3440..4399/label="ORF frame 2"promoter 4448..4477/label="lac_promoter"misc_feature 4491..4513/label="M13_pUC_rev_primer"promoter 4512..4530/label="M13_reverse_primer"CDS 4522..81/label="ORF frame 1"misc_feature 4539..4694/label="lacZ_a"promoter complement(4542..4558)/label="M13_forward20_primer"misc_feature complement(4551..4573)/label="M13_pUC_fwd_primer"pGEX6P1载体基本信息出品公司: GE别名: pGEX-6P-1, pGEX6P1, pGEX 6P 1 质粒类型: 大肠杆菌蛋白表达载体表达水平: 高拷贝启动子: Tac克隆方法: 多克隆位点，限制性内切酶载体大小: 4984 bp5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG 3' 测序引物及序列: pGEX3': CCGGGAGCTGCATGTGTCAGAGG 载体标签: N-GST载体抗性: Ampicillin 氨苄备注: 复制子是pMB1产品目录号: 27-4597-01稳定性: 瞬时表达 Transient组成型: 诱导表达病毒/非病毒: 非病毒pGEX6P1载体质粒图谱和多克隆位点信息pGEX6P1载体简介pGEX6P1载体序列LOCUS pGEX-6P-1 4984 bp DNA circular SYN DEFINITION pGEX-6P-1ACCESSIONKEYWORDSSOURCEORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI/COMMENT VNTAUTHORNAME||FEATURES Location/Qualifierssource 1..4984/organism="pGEX-6P-1"/mol_type="other DNA"promoter 184..212/label="tac_promoter"misc_feature 224..246/label="M13_pUC_rev_primer"gene 258..992/label="GST"/gene="GST"CDS 258..992/label="ORF frame 3"misc_feature 918..938/label="precision"misc_feature complement(1034..1056)/label="pGEX_3_primer"promoter 1322..1350/label="AmpR_promoter"gene 1392..2252/label="Ampicillin"/gene="Ampicillin"CDS 1392..2252/label="ORF frame 3"rep_origin 2407..3026/label="pBR322_origin"misc_feature 3324..4415/label="lacI"CDS 3456..4415/label="ORF frame 3"promoter 4464..4493/label="lac_promoter"misc_feature 4507..4529/label="M13_pUC_rev_primer"promoter 4528..4546/label="M13_reverse_primer"CDS 4538..81/label="ORF frame 2"misc_feature 4555..4710/label="lacZ_a"promoter complement(4558..4574)/label="M13_forward20_primer" misc_feature complement(4567..4589)/label="M13_pUC_fwd_primer"pET28a载体基本信息出品公司: EMD Biosciences (Novagen)别名: pET28a, pet 28a, pET-28a(+) 质粒类型: 大肠杆菌蛋白表达表达水平: 高克隆方法: 多克隆位点，限制性内切酶载体大小: 5369 bp5' 测序引物及序列: T7: 5'-TAATACGACTCACTATAGGG-3' 3' 测序引物序列: T7t: 5'-GCTAGTTATTGCTCAGCGG-3' 载体标签: N-His, N-Thrombin, N-T7, C-His载体抗性: 卡那备注: N端含有Thrombin蛋白酶切位点; pET28a, b, c 的差异仅仅存在于多克隆位点处。