水稻转化培养基组份
N6D2: 1L pH5.8
1)N6 Nutrient Stock
N6大量(20X) 50ml
N6微量(20X) 50ml
2) Iron Stock (100X) 10ml
3) Vitamine Stock
烟酸1mg/ml 0.5ml
VB1 1mg/ml 0.1ml
VB6 1mg/ml 0.5ml
Glycine 2mg/ml 1ml
肌醇50mg/ml 2ml
4) Sucrose 30g
5) 2,4-D 2mg/ml 1ml
6) Casein Enzymatic Hydrolysat 1g/l
7) Phytagel 2.0—2.5g/l
N6D2C (for co-culturing)
N6D2 medium
Glucose 10g/l
AS (20mg/ml) to 20mg/l final conc.
N6D2S1（for selecting）
N6D2 medium
Hygromycin to 25mg/l final conc.
Cefotaximine to 300—400mg/l final conc. (现用300mg/l)
N6D2S2 (for selecting)
N6D2 medium
Hygromycin to 50mg/l final conc.
Cefotaximine to 200mg/l final conc.
预分化
200ml ddH2O中加入0.5g phytagel, 灭菌
再依次加入Higrow Rice Medium 50X Salts I, II, III 各4ml,
微波炉加热消毒灭菌, 加入hygromycin 50mg/l,
Cefotaximine 200mg/l.
分化培养基MSRCH pH 5.8
1)MS
2)0.25g/l 水解酪蛋白CH (可不加)
3)Sucrose 30g/l
4)phytagel 2.5g/l
5)激素终浓度
6-BA 2mg/l
NAA 0.2mg/l
Zeatin 0.2mg/l
KT 0.5mg/l
6)Hygromycin 50mg/l
Cefotaxime 200mg/l
*MS 培养基1L
1. MS大量(20X) 50ml
MS微量(100X) 10ml
2. Fe-EDTA (100X) 10ml
3. Vitamine
烟酸0.5mg/l
VB6 0.5mg/l
VB1 0.1mg/l
Glycine 2mg/l
肌醇100mg/l
1/2MS培养基(生根培养基) 1L pH5.8
1. 1/2MS盐分
MS大量(20X) 25ml
MS微量(100X) 5ml
Fe-EDTA (100X) 5ml
2. MS维生素
3. 多效唑1mg/l
4. NAA 0.5mg/l
5. Sucrose 30g/l
6. Phytagel 2.g
7. Hygromycine 50mg/l
AAM培养基1L pH5.2 8磅灭菌
1. AA大量(20X) 50ml
2. AA微量(100X) 10ml
3. Fe-EDTA (100X) 10ml
4. Vitamine
烟酸0.5mg/l
VB6 0.5mg/l
VB1 1mg/l
Glycine 2mg/l
肌醇100mg/l
5. AA
L-Gln 880mg/l
L-Asp 270mg/l
L-Arg 230mg/l
L-Gly 80mg/l
6. CH (水解酪蛋白) 500mg/l 可不加
7. Sucrose 68.5g/l
8. Glucose 36g/l
AB 1L pH7.2 8磅灭菌
AB大量(20X) 50ml
AB微量(100X) 10ml
Fe-EDTA (100X) 1ml
Glucose (无水) 5g
*N6培养基母液：
Nutrient Stock: (20X) Stock conc. Final conc.
大量: KNO3 56.6g/l 2.83 g/l (NH4)2SO4 9.26g/l 463 mg/l
KH2PO4 8.0 g/l 400 mg/l
MgSO4.7H2) 3.7 g/l 185 mg/l
CaCl2.2H2O 3.3 g/l 166 m g/l
微量: MnSO4.H2O 66 mg/l 3.3 mg/l ZnSO4.7H2O 30 mg/l 1.5 mg/l
H3BO3 32 mg/l 1.6 mg/l
KI 16 mg/l 0.8 mg/l
*Iron Stock (100X)
FeSO4.7H2O 2.8 g/l 28 mg/l
Na2EDTA 3.7 g/l 37 mg/l
Macronutrients (20x): g/L
*独立溶解后加入。

Micronutrients (100x) : mg/L。