APB40
240.00 0.00 160.00 210.00 220.00 43.60 4.93 1.45
0.97 119.00
APB60
160.00 0.00 240.00 200.00 220.00 49.30 7.91 2.24
1.60 119.00
APB80
80.00 0.00 320.00 200.00 220.00 40.60 10.34 2.94
National Aqua-feed Safety Assessment Station, FRI, CAAS,Beijing, P. R. CHINA
Japanese sea bass (Lateolabrax japonicus)
☻ Carnivorous ☻ Widely cultured in both salty and
fresh water ☻ Highest production among marine
species in China.
Background and Justification
Fishmeal substitution: Supply, price, sustainability Animal protein blend: Amino acid complementation, cost savings, sustainability, Palatability for Carnivorous fish species (vs. plant sources) DIAA: More precise than determined AA level, lower crude protein formulation, reducing waste,
Hybid striped bass Menhaden FM,560,Smi-purified diets Pet-food grade PBM Met,Lys
100% Gaylord & Rawles（05）
Hybid striped bass Menhaden FM,250,Commercial diets Pet-food grade PBM Met,Lys
PBM: MBM: BM: HFeM=40:35:20:5 • Substitution level: 20%、40%、60%、80%
of PFM
Formulation and proximate composition of experimental diets (g.kg-1,wet basis)
Ingredients
Peru fish meal Local fish meal Animal protein blend Soybean meal
PFM
400.00 0.00 0.00
210.00
Wheat flour
220.00
Fish oil
50.00
L-lysine(50%) 0.00
DLmethionine(98%)
35%
Rawles et al.（06）
Ｈumpback grouper White FM,694,pratical test diets Pet-food grade PBM /
50%
Shapawi et al.（07）
Malabar grouper Herring meal,500，pratical test diets MBM+BM+PBM+FeM Met
<50% Wang ea al.(08)
Jpanese seabass White FM,400，pratical test diets MBM+BM+PBM+FeMl Met,Lys,Thr <50% Unpub. in our lab (08)
Many factors such as culturing system, husbandry, fish species would affect the trial conclusions, besides, the quality and quantity of the FM used in the control are the most important!
Materials & methods
IBW: 76.2±0.08g Positive Contorl: Peru FM（PFM） Negative Contorl: Local FM（LFM）with high VBN (148mg/kg) Alternative protein resources: Animal protein blend (APB)
3
Literatures review
Fish
FM in control(g.kg-1)
Rep. candidates CAA suplemets Rep. level
Sources
Grouper
Chilean FM,400,pratical test diets MBM+BM
/
80%
Millamena (02)
Moisture( %)
57.25
55.46 81.04
86.03 79.59
Ash( % )
101.44 119.26n( % )
443.40 449.80 434.70
434.40 434.30
Gross energy(MJ· kg-1) 195.20 197.10 190.60
2.07 124.00
Formulation and proximate composition of experimental diets (g.kg-1,wet basis)
Items
PFM
LFM
APB20 APB40 APB60 APB80
Proximate analysis (wet basis)
0.00
L-threonine(98%) 0.00
Others
120.00
LFM
0.00 500.00 0.00 110.00 220.00 42.00 0.00 0.00
0.00 128.00
APB20
320.00 0.00 80.00 210.00 220.00 46.30 2.46 0.72
0.49 120.00