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常用荧光染料探针列表.doc

这是来自于 Salk 的一个比较全的荧光染料列表,这些荧光染料可广泛用于流式细胞术以及荧光显微镜技术,汇集了各种荧光染料的特性,方便大家查找。

可根据实际所用的检测平台、染料的最大激发光波长和最大发射光波长来选择合适的荧光染料用于实验。

请注意这上面所显示的颜色可能会由于所用浏览器不同而有所不同,他们只是一个与实际颜色的近似值。

染料名称Excitation(n Emission(分子量备注信息m) nm)Reactive and conjugated probesHydroxycoumarin 325 386 331 Succinimidyl ester Aminocoumarin 350 445 330 Succinimidyl ester Methoxycoumarin 360 410 317 Succinimidyl ester Cascade Blue (375);401 423 596 Hydrazide Pacific Blue 403 455 406 Maleimide Pacific Orange 403 551Lucifer yellow 425 528NBD 466 539 294 NBD-XR-Phycoerythrin (PE) 480;565 578 240 kPE-Cy5 conjugates 480;565;650 670 aka Cychrome, R670, Tri-Color, Quantum RedPE-Cy7 conjugates 480;565;743 767Red 613 480;565 613 PE-Texas RedPerCP 490 675 Peridinin chlorphyll protein TruRed 490,675 695 conjugateFluorX 494 520 587 GE Healthcare Fluorescein 495 519 389 FITC; pH sensitive BODIPY-FL 503 512TRITC 547 572 444 TRITCX-Rhodamine 570 576 548 XRITCLissamine Rhodamine570 590BTexas Red 589 615 625 Sulfonyl chloride Allophycocyanin (APC)650 660 104 kAPC-Cy7 conjugates 650;755 767 PharRedAlexa Fluor 系列荧光染料Alexa Fluor 350 343 442 410Alexa Fluor 405 401 421 1028Alexa Fluor 430 434 540 702Alexa Fluor 488 499 519 643 QYAlexa Fluor 500 503 525 700Alexa Fluor 514 517 542 714Alexa Fluor 532 530 555 724 QYAlexa Fluor 546 561 572 1079 QYAlexa Fluor 555 553 568 1250 QYAlexa Fluor 568 579 603 792 QYAlexa Fluor 594 591 618 820 QYAlexa Fluor 610 610 629 1285Alexa Fluor 633 632 648 1200Alexa Fluor 647 652 668 1300 QYAlexa Fluor 660 663 691 1100Alexa Fluor 680 680 702 1150Alexa Fluor 700 696 719 1400Alexa Fluor 750 752 776 1300Alexa Fluor 790 782 804 1750Cy 系列荧光染料Cy2 489 506 714 QYCy3 (512);550 570;(615)767 QYCy3B 558 572;(620)658 QY581 594;(640)1102 QYCy5 (625);650 670 792 QY675 694 1128 QYCy7 743 767 818 QY核酸相关探针染料Hoechst 33342 343 483 616 AT-selectiveDAPI 345 455 AT-selectiveHoechst 33258 345 478 624 AT-selectiveSYTOX Blue 431 480 ~400 DNAChromomycin A3 445 575 CG-selective Mithramycin 445 575YOYO-1 491 509 1271Ethidium Bromide 493 620 394Acridine Orange 503 530/640 DNA/RNASYTOX Green 504 523 ~600 DNAVital stain, TOTO: Cyanine TOTO-1, TO-PRO-1 509 533 DimerTO-PRO: Cyanine Monomer Thiazole Orange 510 530Propidium Iodide (PI) 536 617LDS 751 543;590 712;607 472 DNA (543ex/712em), RNA (590ex/607em)7-AAD 546 647 7-aminoactinomycin D, CG-selectiveSYTOX Orange 547 570 ~500 DNA TOTO-3, TO-PRO-3 642 661DRAQ5 647 681,697 413 usable excitation down to 488细胞功能探针Indo-1 361/330 490/405 1010 AM ester. Low/High Ca++, Fluo-3 506 526 855 AM ester. pH > 6DCFH 505 535 529 2'7'Dichorodihydrofluoresce in, oxidized form Dihydrorhodamine 123,DHR 505 534 346 oxidized form,light catalyzes oxidation SNARF 548/579 587/635 pH 6/9荧光蛋白QY BR PSY66H 360 442Y66F 360 508EBFP 380 440 monome rEBFP2 383 448 20 monome rAzurite 383 447 15 monome rGFPuv 385 508T-Sapphire 399 511 26 25 weak dimerCerulean 433 475 27 36 weak dimermCFP 433 475 13 64 monome rECFP 434 477 3CyPet 435 477 18 59 weak dimerY66W 436 485mKeima-Red 440 620 3 monome rTagCFP 458 480 29 dimer AmCyan1 458 489 29 tetramer mTFP1 462 492 54 dimerS65A 471 504Midoriishi Cyan 472 495 25 dimer Wild Type GFP 396,475 508 26kS65C 479 507TurboGFP 482 502 26 k 37 dimerTagGFP 482 505 34 monome rS65L 484 510weakEmerald 487 509 39 dimer; (Invitroge n)S65T 488 511EGFP 488 507 26k 34 174 weak dimerAzami Green 492 505 41 monome rZsGreen1 493 505 105k 40 tetramerTagYFP 508 524 47 monome rEYFP 514 527 26k 51 60 weak dimerTopaz 514 527 57 monome rVenus 515 528 53 15 weak dimermCitrine 516 529 59 49 monome rYPet 517 530 80 49 weak dimerTurboYFP 525 538 26 k dimer ZsYellow1 529 539 13 tetramerKusabira Orange 548 559 31 monome rmOrange 548 562 49 9 monome rmKO 548 559 31 122 monomer TurboRFP 553 574 26 k 62 dimertdTomato 554 581 95 98 tandem dimerTagRFP 555 584 50 monome rDsRed monomer 556 586 ~28k 16 monomer DsRed2 ("RFP") 563 582 ~110k 24mStrawberry 574 596 26 15 monome rTurboFP602 574 602 26 k 26 dimer AsRed2 576 592 ~110k 13 tetramermRFP1 584 607 ~30k monome rJ-Red 584 610 13 dimermCherry 587 610 16 96 monome rHcRed1 588 618 ~52k dimer Katusha 588 635 23 dimermKate (TagFP635) 588 635 15 monome rTurboFP635 588 635 26 k 22 dimer mPlum 590 649 53monomer; faster mRaspberry 598 625 13 photobleach thanmPlum 其他探针Monochlorobimane 380 461 226 Glutathione probe Calcein 496 517 623 pH > 5Ex: Peak excitation wavelength (nm)Em: Peak emission wavelength (nm)QY: Quantum yieldBR: Brightness; Extinction coefficient * Quantum yield / 1000PS: Photostability; time to 50% brightness (sec)光色波长λ(nm)代表波长红( Red) 780 ~ 630700橙( Orange) 630 ~ 600620黄( Yellow ) 600 ~ 570580绿( Green) 570 ~ 500550青( Cyan) 500~ 470500蓝( Blue) 470~ 420470紫( Violet ) 420~ 380420。

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