The term “enzyme” was coined by Wilhelm Kuhne.创造
crystallized 结晶centrifugal supernatant
离心上清液
were used to imitate enzymes mesoporous介孔材料，多孔
DMF-2甲基甲酰胺
Polymer with enzyme-like activity 聚合物
noble-metal nanoclusters 贵金属纳米团簇
In the presence of dopamine在多巴胺的存在下
Molecularly imprinted polymers were invented by 分子印迹聚合物
Nano ceria as superoxide dismutase mimic. 纳米二氧化铈作为超氧化物歧化酶模拟。

peroxidase mimic. 过氧化物模拟酶catalase,过氧化氢酶
Nano ceria as catalase and oxidase mimic纳米二氧化铈过氧化氢酶和氧化酶模拟
metal sulfide 硫化物 cobalt porphyri n钴 manganese锰facile温和的 chemicals and reagent s化学药品和试剂syring注射器 thioacetamide 硫代乙酰胺 stretching vibration 伸缩振动峰 carbonyl group羰基 aromatic acid 芳香酸 calibration curve 校准线
have been extensively explored to mimic the structures and functions of natural enzymes through various approaches.and several monographs andnumerous excellent reviews have been published have been found to exhibit unexpected enzyme-like
Although the progress and achievements of classic artificial enzymes have been thoroughly reviewed in the literature,
no comprehensive review has been devoted to nanozymes
we discuss the current challenges facing nanozyme technologies and future directions to realize their great potential.
新方法
Hybrid materials（杂化材料） formed by incorporating inorganicmaterials into a polymeric matrix have even more promisingadvantages, such as novel
functionalities, enhanced biocompatibility and improved mechanical and chemical properties.
Compared with natural enzymes and organic catalysts,the efficiency of most nanozymes are still lower. Therefore, the development of high performance nanozymes will be a hottopic for future research.
Following these early works, we found that the
pH-tunable oxidation ability of nanoceria to ferrocenecarboxylic acid (Fc-COOH).51
CeO2 nanoparticles, which exist in a mixed valence state (Ce3+, Ce4+), possess many unique properties that have proven to be of high utility in biomedical and catalytic applications.
8,52−57 Recently
Signi fi cant activity enhancement indicated that GO played an important role in modulating the catalytic activity of the AuNCs.
综述
In this Account, we focus on our recent progress in exploring and constructingsuch nanoparticulate arti fi cial enzymes,
We expect that nanozymes with unique properties and
functions will attract increasing research interest and lead to new opportunities in various fi elds of research.
This colorimetric “readout”o ff ers great advantages of simple operation process, low-costportable instrument, and easy-to-use applications.29
In addition to carbon-based nanomaterials, metal nanomaterialshave also been explored to mimic the functions of naturalenzymes.13
Recently, noble-metal nanoclusters have also become a burgeoning area of scienti fi c interest.
Our fi ndings pave the way to applying ionic liquid as a positive modulator in nanozyme-based catalytic reactions nanozyme-based catalytic reactions.
Compared with other nanomaterial-based peroxidase mimics,AuNCs as nanozymes are more prominent for bioanalysis due to their small size, excellent stability, and biocompatibility.50
Herein, we demonstrate the proof of concept of using
CoFe-LDHs nanoplates as an effective peroxidase mimetic to catalyze oxidation of peroxidase
substrate ,30,5,50-tetramethylbenzidine (TMB) in the presence of H2O2 to produce blue color solution
X-ray Diffraction:After sorption experiment, all samples were characterized by powder X-ray diffraction.
Nuclear magnetic resonance (NMR)：
UV-vis absorption spectra：To further demonstrate the stability of PCN-222(Fe), we employed UV-Vis spectrometry to detect the remaining solutions after different treatments
The absorption spectra of AuNPs and HRP-AuNPs were determined with a HITACHI
U-3010 UV-Vis spectrophotometer.
infrared (IR): The Fourier transform infrared spectroscopy (FTIR) measurements were carried out with a Thermo Nicolet Avatar 370 FTIR spectrophotometer. TEM:The morphology of AuNPs was characterized with a Philips CM300 FEG transmission electron microscopy.。