ATCC® Number:CRL-2865™Price: $329.00Designations: T47D-KBlucDepositors: VS WilsonBiosafety Level: 1Shipped: frozenMedium &Serum:See PropagationGrowthProperties:adherentOrganism: Hom o sapi ens (human)Morpholog y: epithelialSource: Organ: mammar y gland; breas tTissue: ductDisease: ductal carcinomaDerived from metastatic site: pleural effusionCell T y p e: epithelialtransfected with reporter plas midPermits/F orms: In addition to the MTA menti oned above, other ATCC and/or regulatory per mits may be required for the transfer of this ATCC material. Any one purchasing ATCC material is ultimatel y r esponsible for obtaining the permits. Please click here for informationregarding the s pecific requirements for shipment to your loc ation.ReverseTranscript:NAge: 54 years adultHeLa Mar kers: NPropagati on: ATCC complet e gro w th medium: T he bas e medi um for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No.30-2001. To mak e the complete growth medium, add the following components to the base medium: 0.2 Units/ml bov ineinsulin; fetal bov i ne s erum to a final c onc entration of 10%.Atmosphere: air, 95%; c arbon diox ide (CO2), 5%Temperature: 37.0°CDuration: The depositor states that suppl ementi ng the growth medi um with ins ulin is optional.Preser v ation: Freeze medium: c ulture medium, 95%; DMSO, 5%Storage temp erature: liquid nitrogen vapor phaseRelatedProduc ts: Recommended medium (without the additional s upplements or s erum described under ATCC Medium):ATCC 30-2001 recommended s erum:ATCC 30-2020parental c ell line:ATCC HTB-1330.25% (w/v ) Try psi n - 0.53 mM EDTA in Hank ' BSS (w/o Ca++, Mg++):ATCC 30-2101Hank s' Balanced Salt Soluti on (w/o Ca++, Mg++, phenol red):ATCC 30-2213实验室常用细胞株来源：<a href='http://www.dxy .c n'>丁香园</a>细胞株名称ATCC 编号细胞来源细胞种类生长状态使用培养基细胞株名称ATCC 编号细胞来源细胞种类生长状态使用培养基293 CRL-1573 人胎肾贴壁、上皮样MEM/NEAA,10%FBS2215 无人肝癌贴壁、上皮样DMEM,15%FBS127TAg CRL-2817 小鼠多能胚胎干细胞胚胎型DMEM, 10% FBS293/CHE-Fc CRL-2368 人肾上皮细胞、贴壁DMEM,10%FBS3T3-L1 CL-173 小鼠胚胎成纤维成纤维DMEM,10%FBS3T6 CCL-96 小鼠胚胎成纤维细胞、贴壁DMEM,10%FBSA431 CRL-1555 人表皮细胞癌上皮细胞、贴壁D MEM,10%FBSA549 CCL-185 人肺癌贴壁、上皮样F12,10%FBSA9 CRL-6319 小鼠结缔组织成纤维细胞、贴壁D MEM,10% FBS+AR42J CRL-1492 大鼠胰腺肿瘤贴壁、上皮样Ham'sF-12K, 20%FBSAtT-20 CCL-89 小鼠垂体肿瘤上皮细胞、贴壁F-10, 15% HS和2.5% FBSB95-8 CRL-2311 绒猴EB病毒感染的白血病细胞成淋样RPMI-1640, 10% FBS BALB/3T3 CCL-163 小鼠胚胎成纤维细胞、贴壁DMEM, 10% FBSbel7402 无人肝癌贴壁、上皮样RPMI-1640,15%FBSBeWo CCL-98 人绒毛癌上皮细胞、贴壁F-12K, 15%F12BHK-21 CCL-10 仓鼠肾成纤维细胞、贴壁MEM/NEAA, 10% FBSBHL-100 无人乳房上皮细胞、贴壁McCoy'5A, 10% FBSBRL 3A CRL-1442 大鼠肝上皮细胞、贴壁MEM/NEAA, 10% FBSBT CRL-1390 牛鼻甲骨细胞成纤维细胞、贴壁DMEM, 10% FBSCaco-2 HTB-37 人结肠腺癌上皮细胞、贴壁MEM/NEAA, 10% FBSChang 无人肝脏上皮细胞、贴壁BME, 10% FCSCHO-K1 CRL-9618 仓鼠卵巢上皮细胞、贴壁F-12, 10%FBSCL2 CRL-2514 小鼠B细胞淋巴细胞、悬浮RPMI-1640, 10% FBSCL3 CRL-2515 小鼠B细胞淋巴细胞、悬浮RPMI-1640, 10% FBSClone 9 CRL-1439 大鼠肝脏上皮细胞、贴壁F-12K, 10%FBSClone M-3 CCL-53.1 小鼠黑素瘤上皮细胞、贴壁F-12, 15% HS和2.5% FBS COS-1 CRL-1650 猴肾成纤维细胞、贴壁DMEM, 10% FBSCOS-3 无猴肾成纤维细胞、贴壁MEM, 10% FBSCOS-7 CRL-1651 猴非洲绿猴肾成纤维细胞、贴壁DMEM, 10% FBSCRFK CCL-94 猫肾上皮细胞、贴壁MEM/NEAA, 10% FBSCV-1 CCL-70 猴非洲绿猴肾成纤维细胞、贴壁MEM/NEAA, 10% FBSD-17 CCL-183 狗骨肉瘤上皮细胞、贴壁MEM/NEAA, 10% FBSDAN CRL-2130 狗骨肉瘤上皮细胞、贴壁MEM/EBSS, 8% FBSDaudi CCL-213 人淋巴瘤病人血液成淋巴细胞、悬浮RPMI-1640, 10% FBSDLD-1 CCL-221 人结肠直肠腺癌上皮细胞、贴壁RPMI-1640, 10% FBSDSDh CRL-2131 狗骨肉瘤上皮细胞、贴壁MEM/EBSS, 8% FBSDSN CRL-9939 狗骨肉瘤上皮细胞、贴壁MEM/NEAA, 10% FBSDu145 HTB-81 人前列腺瘤贴壁、上皮样MEM/NEAA,10%FBSEB-3 CCL-85 人Burkitt淋巴瘤淋巴样、悬浮RPMI-1640, 10% FBSES-D3 CRL-1934 小鼠多能胚胎干细胞胚胎型DMEM+ 15% FBSF9 CRL-1720 小鼠睾丸畸胎癌胚胎型至内胚层DMEM, 15% FBSGH1 CCL-82 大鼠垂体肿瘤上皮细胞、贴壁F-12, 15% HS和2.5% FBSGH3 CCL-82.1 大鼠垂体肿瘤上皮细胞、贴壁F-12, 15% HS和2.5% FBSH9 HTB-176 人T细胞淋巴瘤成淋巴细胞、悬浮RPMI-1640, 10% FBSH9/HTLV-IIIB CRL-8543 人T细胞淋巴瘤成淋巴细胞、悬浮RPMI-1640, 10% FBS HaK CCL-15 仓鼠肾上皮细胞、贴壁BME, 10%FCSHCT-15 CCL-225 人结肠直肠腺癌上皮细胞、贴壁RPMI-1640, 10% FBSHela CCL-2 人宫颈癌贴壁、上皮样MEM/NEAA,10%FBSHela S3 CCL-2.2 人子宫颈癌贴壁、上皮样F12,10%FBSHep-2 无人喉癌上皮细胞、贴壁MEM, 10% FBS;或RPMI-1640, 10% FBS Hep3B HB8064 人肝癌贴壁、上皮样MEM/NEAA,10%FBSHepG2 HB-8065 人肝癌贴壁、上皮样MEM/NEAA,10%FBSHK-2 CRL-2190 人肾皮质贴壁、上皮样Keratinocy te-Serum Free Medium + 5 ng/ml rhEGF HL-60 CCL-240 人急性早幼粒细胞白血病悬浮、粒细胞样RPMI-1640,20%FBSHP [HT1080 poly] CRL-12012 人纤维肉瘤上皮细胞、贴壁D MEM, 10% FBSHT-1080 CCL-121 人纤维肉瘤上皮细胞、贴壁MEM/NEAA, 10% FBSHT-29 HTS-38 人腺癌贴壁、上皮样DMEM/F12(1:1),5%FBSHTC ICLC 收录ATL95006 大鼠肝脏贴壁、上皮样MEM/EBSS,10%FBSHuT 78 TIB-161 人T细胞淋巴瘤成淋巴细胞、悬浮IMD M, 20% FBSHUVEC 无人脐带内皮细胞、贴壁F-12K, 10% FBS 和肝素盐100 ug/mlHX [HT1080 xeno] CRL-12011 人纤维肉瘤上皮细胞、贴壁D MEM, 10% FBS细胞株名称ATCC 编号细胞来源细胞种类生长状态使用培养基I-10 CCL-83 小鼠睾丸癌上皮细胞、贴壁F-10, 15% HS和2.5% FBSIEC-6 CRL-1592 大鼠正常小肠上皮样、贴壁DMEM, 5% FBS,胰岛素0.1μg/mlIM-9 CCL-159 人骨髓瘤病人骨髓成淋巴细胞、悬浮RPMI-1640, 10% FBSIMR-90 CCL-186 人肺成纤维细胞、贴壁MEM/NEAA, 10% FBSJAA-F11 CRL-2381 小鼠B细胞淋巴样、悬浮DMEM,10% FBS和NEAAJEG-3 HTB-36 人绒毛膜癌上皮细胞、贴壁MEM/NEAA, 10% FBSJensen CCL-45 大鼠肉瘤成纤维细胞、贴壁McCoy's 5A, 5% FBSJurkat TIB-152 人急性T淋巴细胞白血病悬浮、淋巴细胞样RPMI-1640,10%FBSK-562 CCL-243 人骨髓性的白血病成淋巴细胞、悬浮RPMI-1640, 10% FBSKB CCL-17 人口腔癌上皮细胞、贴壁MEM/NEAA, 10% FBSKG-1 CCL-246 人红白血病病人骨髓骨髓白细胞、悬浮IMDM, 20% FBSKG-1a CCL-246.1 人红白血病病人骨髓骨髓白细胞、悬浮IMDM, 20% FBSL2 CCL-149 大鼠肺上皮细胞、贴壁F-12K, 10%FBSL6 CRL-1458 大鼠骨骼肌成肌细胞贴壁D MEM, 10% FBSLLC-WRC 256 CCL-38 大鼠癌上皮细胞、贴壁199培养基, 5% HSLnCap CRL-1740 人前列腺癌贴壁、上皮样RPMI-1640,10%FBSLO2 无人正常肝细胞贴壁、上皮样DMEM,15%FBSM. dunni (Clone III8C) CRL-2017 小鼠皮肤成纤维细胞、贴壁McCoy's 5a , 10% FBSMARC S5 CRL-2507 小鼠B细胞悬浮、淋巴细胞样MEM/NEAA或DMEM, 20%FBS(灭活)MCF7 HTB-22 人乳腺癌上皮细胞、贴壁MEM/NEAA, 10% FBS10ug/ml 胰岛素MHCC97 无人肝癌贴壁、上皮样D MEM,15%FBSMOLT-4 CRL-1582 人T淋巴细胞、急性淋巴细胞白血病悬浮、淋巴细胞样RPMI-1640,10%FBS NIH3T3 CRL-1658 小鼠胚胎贴壁、成纤维细胞D MEM,15%FBSNK-92 CRL-2407 人淋巴瘤成淋巴细胞、悬浮α-MEM,12.5% HS和12.5% FBSNK-92MI CRL-2408 人淋巴瘤成淋巴细胞、悬浮α-MEM,12.5% HS和12.5% FBSPANC-1 CRL-1469 人胰腺癌贴壁、上皮样DMEM,10%FBSPC12 CRL-1721 大鼠肾上腺嗜铬细胞瘤多角型、松散贴壁、易结团生长F12 ,15% HS和2.5%FBS PC3 CRL-1435 人前列腺腺癌早期、骨转移贴壁、上皮样F12,10%FBS细胞株名称ATCC 编号细胞来源细胞种类生长状态使用培养基Raji CCL-86 人Burkitt淋巴瘤淋巴样、悬浮RPMI-1640, 10% FBSRat2 CRL-1764 大鼠Rat2(TK-)克隆衍生成纤维细胞样、贴壁DMEM, 5% FBS,30μg/ml BrdURS4;11 CRL-1873 人急性淋巴细胞白血病成淋巴细胞、悬浮RPMI-1640, 10% FBSSaos-2 HTB-85 人骨肉瘤上皮细胞、贴壁McCoy'5A, 15% FBSSMMC7721 无人肝癌贴壁、上皮样RPMI-1640,15%FBSSP2/0 ACC146 小鼠杂交瘤悬浮IMDM,7.5%FBSSW480 CCL-228 人结肠癌贴壁、上皮样10%FBS,L15SW620 CCL-227 人结肠癌贴壁、上皮样10%FBS+2m M L-GLU+90%L15THP-1 TIB-202 人急性单核系白血病、血液悬浮、单核细胞样RPMI-1640,10%FBSU937 CRL-1593.2 人淋巴浆母细胞瘤悬浮、单核细胞样RPMI-1640,10%FBSVero CCL-81 非洲绿猴肾贴壁、上皮样DMEM,10%FBSVero-E6 CRL-1586 非洲绿猴肾贴壁、上皮样MEM/EBSS,10%FBSWEHI-3 TIB-68 小鼠骨髓单核细胞白血病类巨噬细胞、悬浮IMDM, 10% FBSWI-38 CCL-75 人胚胎肺上皮细胞、贴壁BME, 10% FBSWISH CCL-25 人羊膜上皮细胞、贴壁BME, 10% FBSWS1 CRL-1502 人胚胎皮肤上皮细胞、贴壁MEM/NEAA 10% FBS WSS-1 [WS-1] CRL-2029 人肾上皮细胞、贴壁DMEM, 10% FBSXC CCL-165 大鼠肉瘤上皮细胞、贴壁MEM, 10% FBS 和NEAAY-1 CCL-79 小鼠肾上腺瘤上皮细胞、贴壁F-10, 15% HS和2.5% FBS 说明：以上数据来源于ATCC,建议使用以上推荐的培养基。