Bpu1102I
FastDigest® BsaAI FastDigest® BsaBI FastDigest® BsaHI FastDigest® BsaJI
BseDI
CCTNAGC GCTNAGC YACGTR GATNNNNA
TC GRCGYC
CCNNGG CCNNGG
BseGI FastDigest® BseGI BseJI
37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 37 °C
T
50-100%
Buffer AarI + oligo
NR
B
100%
T
50-100%
F
F
O
0-20%
F
F
F
F + SAM
0-20% 100% 20-50%
0-20% 20-50%
100%
20-50%
50-100% 50-100% 50-100% 50-100%
0-20%
0-20%
100%
20-50%
F + SAM
F Buffer Bpu10I
F
T
F
0-20% 20-50% 50-100% 100% 50-100% 100%
BspPI
GGATC
BspTI FastDigest® BsrBI FastDigest® BsrDI FastDigest® BsrFI FastDigest® BssHII Bst1107I
BstXI
FastDigest® BstXI FastDigest® BstZ17I Bsu15I FastDigest® Bsu36I BsuRI
AGCT
Alw21I
GWGCWC
FastDigest®
Alw21I
GWGCWC
Alw26I
GTCTC
FastDigest® Alw26I
GTCTC
Alw44I
GTGCAC
FastDigest® CAGNNNCT
AlwNI
G
ApaI
GGGCCC
FastDigest® ApaI
GGGCCC
FastDigest® ApaLI
100% 100%
50-100% 0-20%
NR
100%
0-20% 20-50% 50-100% 50-100%
F T T Buffer BfuI
O
20-50% 0-20%
NR
0-20%
20-50% 50-100%
NR
50-100%
0-20% 0-20% 0-20%
100%
0-20% 0-20% 0-20%
内切酶
识别位点 孵育温度 推荐缓冲液 B
AanI
TTATAA
AarI
CACCTGC
AasI
AatII FastDigest® AatII FastDigest® AccI Acc65I FastDigest® Acc65I FastDigest® AciI FastDigest® AclI FastDigest® AcuI
0-20%
0-20% 0-20% 50-100% 0-20% 50-100% 50-100% 100%
BamHI
FastDigest® BamHI FastDigest® BanI BauI
GGATCC GGATCC GGYRCC CACGAG
37 °C Buffer BamHI 20-50%
37 °C
100% 100%
FastDigest® BbsI FastDigest® BbvI
BclI
FastDigest® BclI
BcnI
GAAGAC GCAGC TGATCA TGATCA CCSGG
BcuI
ACTAGT
BdaI
FastDigest® BfaI BfiI
TGANNNNN NTCA CTAG
F
37 °C
F
37 °C
T
0-20%
G 50-100%
NR 20-50% 20-50% 20-50%
100%
NR 50-100%
0-20% 0-20% 0-20% 20-50% 100% 100% 20-50%
100% 50-100%
在各种Fermentas缓冲液中的活性
O 0-20%
R 0-20%
50-100% 50-100% 20-50% 20-50%
100%
20-50%
F
F
F
T
50-100% 20-50% 0-20%
0-20%
100% 50-100%
T
20-50% 50-100% 20-50% 20-50%
100%
20-50%
F
O
NR
100%
100%
NR
NR
100%
T
20-50%
100% 50-100% 20-50%
50-100% 50-100%
0-20% 0-20% 0-20%
100% 100% 0-20%
0-20% 20-50%
100%
20-50% 100%
20-50%
100% 50-100% 20-50% 50-100%
0-20%
0-20%
100%
100%
0-20% 50-100% 100% 50-100%
50-100%
50-100% NR
50-100% 50-100% 20-50%
0-20% NR
F
R
0-20%
0-20% 50-100% 100%
20-50% 50-100%
100% 100%
100%
100% 100% 100% 100% 100%
100% 100% 100% 100% 100% 100% 100% 100%
T 100%
0-20%
0-20%
NR
2xT 20-50% 50-100%
0-20% 0-20%
0-20% 0-20%
50-100% 100%
0-20% 20-50%
100%
20-50% 20-50% 50-100%
20-50%
100%
100%
20-50%
20-50% 20-50%
NR 100%
NR
20-50%
37 °C
37 °C
37 °C 37 °C 37 °C 37 °C 37 °C 55 °C 37 °C 37 °C 55 °C 37 °C 37 °C 37 °C 55 °C
AdeI
FastDigest® AfeI FastDigest® AflII FastDigest® AgeI AjiI
GACNNNNN NGTC
GACGTC GACGTC
GTMKAC GGTACC GGTACC
CCGC
AACGTT
CTGAAG CACNNNGT
G AGCGCT
CTTAAG
ACCGGT CACGTC
GTGCAC
FastDigest® AscI
GGCGCGCC
FastDigest® AseI
ATTAAT
FastDigest®
AsiSI
GCGATCGC
FastDigest®
AvaI
CYCGRG
FastDigest® AvaII
GGWCC
FastDigest®
AvrII
CCTAGG
37 °C
37 °C
0-20%
0-20% 20-50% 0-20%
20-50% 50-100% 100% 50-100% 0-20% 50-100% 100% 50-100%
F
100% 100% 100% 100% 100% 100%
100% 100% 100%
100% 100%
100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100% 100%
37 °C 37 °C 55 °C 37 °C 37 °C 37 °C 30 °C 37 °C 37 °C 37 °C 37 °C 37 °C
37 °C 37 °C 37 °C 37 °C 37 °C
37 °C
37 °C 37 °C 37 °C 37 °C
37 °C 37 °C 37 °C 37 °C 37 °C 37 °C 65 °C 37 °C 37 °C 55 °C 55 °C 37 °C 65 °C
ACTGGG
BfmI
CTRYAG
BfuI
GTATCC
BglI
FastDigest® BglI
BglII FastDigest® BglII FastDigest® BlpI Bme1390I
GCCNNNNN GGC
GCCNNNNN GGC
AGATCT
AGATCT
GCTNAGC
CCNGG
FastDigest® Bme1580I
55 °C 55 °C 55 °C 65 °C 65 °C 55 °C 65 °C 65 °C 37 °C
F
2xT G F T T
G + SAM
20-50%
100%
20-50% 20-50%
100%
100% 100%
20-50% 50-100% 50-100% 50-100% 50-100% 50-100% 0-20% 20-50%