SANTA CRUZ BIOTECHNOLOGY,INC.ER α(MC-20):sc-542Santa Cruz Biotechnology,Inc. 1.800.457.3801831.457.3800fax 831.457.3801Europe +BACKGROUNDEstrogen receptors (ER)are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors.Estrogen receptors,including ER αand ER β,contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues.ER αand ER βhave been shown to be differentially activated by vari-ous ligands.Receptor-ligand interactions trigger a cascade of events,including dissociation from heat shock proteins,receptor dimerization,phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes.Evidence suggests that ER αand ER βmay be regulated by distinct mechanisms even though they share many functional characteristics.REFERENCES1.Danielian,P .S.,et al.1992.Identification of a conserved region required for hormone dependent transcriptional activation by steroid hormone receptors.EMBO J.11:1025-1033.2.Mosselman,S.,et al.1996.ERb:identification and characterization of a novel human estrogen receptor.FEBS Lett.392:49-53.CHROMOSOMAL LOCATIONGenetic locus:ESR1(human)mapping to 6q25.1;ESR1(mouse)mapping to 10A1.SOURCEER α(MC-20)is an affinity purified rabbit polyclonal antibody raised against a peptide mapping at the C-terminus of ER αof mouse origin.PRODUCTEach vial contains 200µg IgG in 1.0ml of PBS with <0.1%sodium azide and 0.1%gelatin.Blocking peptide available for competition studies,sc-542P ,(100µg peptide in 0.5ml PBS containing <0.1%sodium azide and 0.2%BSA).Available as TransCruz reagent for Gel Supershift and ChIP applications,sc-542X,200µg/0.1ml.Available as fluorescein (sc-542FITC)or rhodamine (sc-542TRITC)conjugates for immunofluorescence,200µg/ml.Available as Alexa Fluor ®405(sc-542AF405),Alexa Fluor ®488(sc-542AF488)or Alexa Fluor ®647(sc-542AF647)conjugates for immunofluores-cence;100µg/2ml.Alexa Fluor ®is a trademark of Molecular Probes,Inc.,Oregon,USASTORAGEStore at 4°C,**DO NOT FREEZE**.Stable for one year from the date of shipment.Non-hazardous.No MSDS required.RESEARCH USEFor research use only,not for use in diagnostic procedures.APPLICATIONSER α(MC-20)is recommended for detection of estrogen receptor αof mouse,rat and human origin by Western Blotting (starting dilution 1:200,dilution range 1:100-1:1000),immunoprecipitation [1-2µg per 100-500µg of total protein (1ml of cell lysate)],immunofluorescence and immunohistochemistry (including paraffin-embedded sections)(starting dilution 1:50,dilution range 1:50-1:500)and solid phase ELISA (starting dilution 1:30,dilution range 1:30-1:3000).Suitable for use as control antibody for ER αsiRNA (h):sc-29305,ER αsiRNA (m):sc-29306,ER αsiRNA (h2):sc-44204,ER αsiRNA (r):sc-45949ER αshRNA Plasmid (h):sc-29305-SH,ER αshRNA Plasmid (m):sc-29306-SH,ER αshRNA Plasmid (h2):sc-44204-SH,ER αshRNA Plasmid (r):sc-45949-SHER αshRNA (h)Lentiviral Particles:sc-29305-V,ER αshRNA (m)Lentiviral Particles:sc-29306-V,ER αshRNA (h2)Lentiviral Particles:sc-44204-V and ER αshRNA (r)Lentiviral Particles:sc-45949-V.ER α(MC-20)X TransCruz antibody is recommended for Gel Supershift and ChIP applications.Molecular Weight of ER α:66kDa.DATASELECT PRODUCT CITATIONS1.Massaad-Massade,L.,et al.2002.HMGA1enhances the transcriptional activity and binding of the estrogen receptor to its responsive element.Biochemistry 41:2760-2768.2.Guo,Z.,et al.2002.Estradiol-induced nongenomic calcium signaling regu-lates genotropic signaling in macrophages.J.Biol.Chem.277:7044-7050.3.McCarthy,T.L.,et al.2003.Runx2integrates estrogen activity in osteo-blasts.J.Biol.Chem.278:43121-43129.4.Perissi,V.,et al.2004.A corepressor/coactivator exchange complex required for transcriptional activation by nuclear receptors and other regulated transcription factors.Cell 116:511-526.5.Omoto,Y.,et al.2005.Estrogen receptor αand imprinting of the neonatal mouse ventral prostate by A 102:1484-1489.6.Pedram,A.,et al.2007.A conserved mechanism for steroid receptor translocation to the plasma membrane.J.Biol.Chem.282:22278-22288.7.Klein,C.,et al.2010.Transcriptional profiling of equine endometrium during the time of maternal recognition of pregnancy.Biol.Reprod.83:102-113.ER α(MC-20):sc-542.Western blot analysis of human recombinant ER α.<ERα116K -80K -52K -35K -30K-ER α(MC-20):sc-542.Immunoperoxidase staining of formalin-fixed,paraffin-embedded human breast carcinoma tissue showing nuclear localization.。