pEGFP-N-3’:
CGT CGC CGT CCA GCT CGA CCA G
pEGFP-C-5’:
CAT GGT CCT GCT GGA GTT CGT G
pEGFP-C-3' :
TAT GGC TGA TTA TGA TCA GT
PBV220F:
AAG AAG GGC AGC ATT CAA AG
M13(-96)
CCC TCA TAG TTA GCG TAA CG
SP6
ATT TAG GTG ACA CTA TAG
T7
TAA TAC GAC TCA CTA TAG GG
T7 terminator
TGC TAG TTA TTG CTC AGC GG
T3
ATT AAC CCT CAC TAA AGG GA
P1
CCA GGC TTT ACA CTT TAT GC
P2
GCG ATT AAG TTG GGT AAC GC
pLEXA-F
CGT CAG CAG AGC TTC ACC AT
pLEXA-R
TAA AAC CTA AGA GTC ACT TT
pLNCX-F
AGC TCG TTT AGT GAA CCG TCA GAT CG
pGEX-4T-5'
GGG CTG GCA AGC CAC GTT TGG TG
pGEX-4T-3'
CCG GGA GCT GCA TGT GTC AGA GG
GLp1
TGT ATC TTA TGG TAC TGT AAC TG
GLp2
CTT TAT GTT TTT GGC GTC TTC CA
RVp3
CTA GCA AAA TAG GCT GTC CC
RVp4
GAC GAT AGT CAT GCC CCG CG
pcDNA3.1R
TAG AAG GCA CAG TCG AGG
PinPoint primer
CGT GAC GCG GTG CAG GGC G
pCMV-F
TCT AAA AGC TGC GGA ATT GT
5'TriplEx2
CTC CGA GAT CTG GAC GAG C
RVP4
GAC GAT AGT CAT GCC CCG CG
RVP3
CTA GCA AAA TAG GCT GTC CC
pQE30-R
GTT CTG AGG TCA TTA CTG G
pQE30-F
TGA GCG GAT AAC AAT TTC AC
GTG GTA TGG CTG ATT ATG ATC AG
MalE Primer
GGT CGT CAG ACT GTC GAT GAA GCC
pCMV-R
TCCAAACTCATCAATGTATC
pTRC99C-F:
TTG CGC A CAT CAT AAC
pTRC99C-R:
CTGCGTTCTGATTTAATCTG
pCEP-F:
AGA GCT CGT TTA GTG AAC CG
EBV-R :
GTG GTT TGT CCA AAC TCA TC
PBV220R:
CTG CGT TCT GAT TTA ATC TG
U6
ATG GAC TAT CAT ATG CTT ACC GTA
2.0rev primer:
AGG CGA TTA AGT TGG GTA
3.0rev:
GAG TTA GCT CAC TCA TTA GGC
S.tag
GAA CGC CAG CAC ATG GAC
pAS2-1.R
AAG AGT TAC TCA AGA ACA AGA A
pFlag-CMV-F:
GGT AGG CGT GTA CGG TGG
pFlag-CMV-R:
GCA CTG GAG TGG CAA CTT
pIRES-F:
CTT ACT GAC ATC CAC TTT GC
pIRES.R:
CAC TGC ATT CTA GTT GTG GT
原文地址：Invitrogen中国测序通用引物序列作者：凤歌
引物名称
序列(5'-3')
M13R
CAG GAA ACA GCT ATG ACC
M13F
TGT AAA ACG ACG GCC AGT
M13F(-47)
CGC CAG GGT TTT CCC AGT CAC GAC
M13R(-48)
AGC GGA TAA CAA TTT CAC ACA GGA
TTC CAA AAT GTC GTA ATA AC
P5'
TGC GTA CTG CGG TGA TCA AC
P3'
CTG CAA GGC GAT TAA GTT GG
BAC2
ACG CAC AGA ATC TAG CGC TT
BAC1
AAC CAT CTC GCA AAT AAA TA
3'BD:
TAA GAG TCA CTT TAA AAT TTG TAT AC
CAA CGT GAA AAA ATT ATT ATT CGC
S6
GTA AAT GAA TTT TCT GTA GTA GG
5`AOX1
GAC TGG TTC CAA TTG ACA AGC
3`AOX1
GCA AAT GGC ATT CTG ACA TCC
α-Factor
TAC TAT TGC CAG CAT TGC TGC
pLNCX-R
ACC TAC AGG TGG GGT CTT TCA TTC CC
pLXSN-F
CTT GAA CCT CCT CGT TCG AC
pLXSN-R
GTT GCT GAC TAA TTG AGA TG
pSHUTTLE-CMV-F
GGT CTA TAT AAG CAG AGG TG
pSHUTTLE-CMV-R
GAL4 AD
TAC CAC TAC AAT GGA TG
pACT2-R
GTGCACGATGCACAGTTGAA
pB42ADF:
CCA GCC TCT TGC TGA GTG GAG ATG
pB42ADR:
AAG CCG ACA ACC TTG ATT GGA G
pEGFP-N-5’:
TGG GAG GTC TAT ATA AGC AGA G
pTarget.F
CCA GGA TTT TCC CAG TCA C
pTarget.R
GGC TTT ACA CTT TAT GCT TC
T7(17base)
ACA TCC ACT TTG CCT TTC TC
pYes2.R
TCG GTT AGA GCG GAT GTG
GAL4 BD
TCA TCG GAA GAG AGT AG
pEF-F
TCA AGC CTC AGA CAG TGG TTC
pDSRED-N1-R
TGA AGC GCA TGA ACT CCT TG
pDsRED-express-C1-F
TCC CAC AAC GAG GAC TAC AC
pCMV5R
ATT ATA GAG GAC ACC TAG TC
pCMV5F
pIRES2-EGFP.P5’:
GTA GGC GTG TAC GGT GGG AG
pIRES2-EGFP.P3’:
AAC GCA CAC CGG CCT TAT TC
3'AD:
AGA TGG TGC ACG ATG CAC AG
CMV -F
CGC AAA TGG GCG GTA GGC GTG
S1