测序通用引物列表测序通用引物列表载体名称上游引物（5'primer）下游引物（3'primer）P3*FLAG-CMV CMV-30(825-854) CMV-24(1129-1152) pAc5.1/V5-His(_A,_B,_C) pAc5-5 BGH rev pAcG2T pGEX-5 not availablepACT T7EEV T3/EBVrevpACT2 GAL4 ADfor pGAL4-ADrvpACT2 p17110 p12584pACYC184(BamHI-site) PBRforBam PBRrevBampACYCDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1pACYCDuet-1(MCSII) DuetUp2 T7-Terminator pAdEasy-1 not available not availablepAdTrack-CMV not available not available pAS2-1 GAL4 Bdfor/P24990 pGAL4-BDrv/P31430pB42AD pB42ADF PB42ADRpBABE pBMN_5'pBacPAK8 BAC1 BAC2pBAD pBAD-F pBAD-R pBAD/HisABC pBAD-5'pBBR1MCS T3/M13rev T7/M13forpBD-GAL4 pBD-GAL4-F PBD-GAL4-RpBI121 35S NOSpBIND GAL4-Bdfor T3,EBVrevpBK-CMV M13F/T7 M13R/T3 pBluescriptIIKS(+/-) T3/M13rev T7/M13for（优先用M13F）pBluescriptIISK(+/-) T3/M13rev T7/M13forpBluescriptKS(+/-) T3/M13rev T7/M13forpBluescriptSK(+/-) T3/M13rev T7/M13forpBR322(BamHI-site) PBRforBam PBRrevBampBR322(EcoRI/HindIII-sites) pBRforEco pBRrevHind pBR325(BamHI-site) PBRforBam PBRrevBampBR329(BamHI-site) PBRforBam PBRrevBam pBridge(MCSI) GAL4-Bdfor 3'BDpBridge(MCSII) not available not available pBS-T II T3/M13rev T7/M13for pBS（AMP）T7 T3 pBSR T3 T7ter pBudCE4.1(MCSI) CMV-Profor,T7 c-mycrev,EBVrevpBudCE4.1(MCSII) EF-1α Fwd BGHpBudCE4.1/lacZ/CAT CMV-Profor,T7 c-mycrev,EBVrev pBV220 pBV220F PBV220RpCAL-c T7/Tac-Profor T7-TerminatorpCAL-n T7/Tac-Profor T7-Terminator pCAMBIA1301(1300) pCAMBIA1301-5'(ECORI)/M13R pCAMBIA1301-3'(HindIII) pCAMBIA1304 pCAMBIA1301-5'(ECORI) pCAMBIA1301-3'(HindIII) pCAMBIA2300 M13R(-48) M13F(-47)/M13F(-49) pCANTB5E S1 S6pCAT3-enhancer RVP3 此载体无反向引物pCDFDuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1pCDFDuet-1(MCSII) DuetUP2 T7TER pcDNA1.1 CMV-Profor,T7 pCDM8-3pcDNA2.1 M13for/T7 M13rev/Tac-ProforpcDNA3 T7/CMV-Profor BGH rev/SP6 pcDNA3.1(+/-) pcDNA3.1F/T7/CMV-Profor BGH rev pcDNA3.1/His(_A,_B,_C) T7/CMV-Profor BGH rev pcDNA3.1/myc-His(_A,_B,_C) T7/CMV-Profor BGH rev/c-mycrev pcDNA3.1/V5-His(_A,_B,_C) T7/CMV-Profor BGH rev/V5rev pcDNA3.1/Hygro(+,-) T7/CMV-Profor BGH rev/V5revpcDNA3.1/Zeo(+.-) T7 BGHrevpcDNA3.3TOPOTA CMV-Profor TKPAreverse pcDNA4/HisMax(_A,_B,_C) Xpressfor BGH revpcDNA4/HisMax-TOPO Xpressfor BGH rev pcDNA4/HisMax-TOPO/lacZ Xpressfor BGH rev pcDNA4/myc-His(_A,_B,_C) T7,CMV-Profor BGH rev,c-mycrev pcDNA4/TO TREfor/CMV-Profor BGH rev pcDNA4/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev pcDNA4-TET/ON/AMP+ CMV-ProforpcDNA5/FRT T7/CMV-Profor BGH rev pcDNA6/myc-His(_A,_B,_C) CMV-Profor,T7 BGH rev,c-mycrev pcDNA6/V5-His(_A,_B,_C) CMV-Profor,T7 BGH rev,V5rev pCEP4 pCEP-F/CMV-Profor EBVrevpCI T7（17BASE）EBVrevpCI-neo T7（17BASE）EBVrev,T3pCMS-EGFP T7 T3pCMS-EGFP T7 EBVrev,T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7 pCMV5 pCMV5F PCMV5RpCMV6 CMV-Profor pCMV6-3、HGHrevpCMV6-XL4 CMV-Profor/T7 pCMV6-3、HGHrevpCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrev pCMV-MYC(AMP+) PCMV-F PCMV-R, EBVrevpCMVmyc/nuc CMV-Profor BGH rev,c-mycrev pcmv-scriptEXVector T3 T7 pCMV-Sport6 T7/M13for SP6/M13rev pCOLADuet-1(MCSI) ACYCDuetUP1 Primer DuetDOWN1 pCOLADuet-1(MCSII) DuetUP2 T7TER pCR2.1 T7,M13for M13rev pCR2.1-TOPO M13for,T7 M13rev pCR3.1 T7 BGH rev pCR3.1-Uni T7 BGH rev pCR4 T3/M13rev T7/M13for pCR4-Blunt T3/M13rev T7/M13forpCR4-TOPO M13rev,T3 M13for,T7pCR-Blunt M13rev T7,M13forpCR-BluntII M13rev/SP6 T7/M13for pCR-BluntII-TOPO M13rev/SP6 M13for/T7 pCRII M13rev/SP6 T7/M13for pCRII-TOPO M13rev/SP6 M13for/T7pCR-ScriptSK(+) T7/M13for T3/M13revpCR-XL-TOPO M13rev T7,M13forpCS2+(MCSI) SP6 EBVrev,T7?pCS2+(MCSII) T3 pSG5-3pDB_GAL4cam T7PpDB_leu PDBLeu-5 PDBLeu-3pDEST22 DEST22F DEST22RpDEST15 pGEX-5 T7-TerminatorpDEST20 pGEX-5 pFastBac-3pDEST26 TREfor/CMV-Profor T7/M13forpDisplay T7/CMV-Profor c-mycrev pDNR-LIB(MCS_A) M13for,T7 pSG5-3,M13R pDonar M13F M13RpDONR201 PDONR-F PDONR-RpDONR207 PDONR-F PDONR-RpDONR223 M13F M13RpDrive T7/M13rev SP6/M13for pDsRED1-C1 (DsRed1-N-f) DsRed1-C-rpDsRed1-N1 CMV-Profor RFP-Nrev,CMV-R?pDsRed2-N1 (DsRed1-N-f) DsRed1-C-rpDsRed2-C1 (DsRed1-N-f) DsRed1-C-r pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’ pDsRed-Express dsRed1_N_primer/CMV-F dsRed1_C_primer/CMV-R pDSRED-monomer-N1(KAN+) dsRed1_N_primerpDSRED-N1(KAN+) pEGFP-N-5’ pDSRED-N-RpEASY-Blunt M13F T7/ M13RpEASY-BluntSimple M13F T7/ M13R（距离插入位点合适，优先安排）pEASY-T1 Simple M13F M13R（距离插入位点合适，优先安排）pEASY-T1 M13F/T7 M13RpEASY-T3 M13F/T7 M13R/SP6pECFP-N(C) PECFP-N-5. PECFP-N-3,pEF/myc/cyto/ER/mito/nuc pEF-5 Pcdna3.1R/BGH rev pEF1(4,6/myc-His) T7/pEF-5 Pcdna3.1R/BGH rev pEF1(4,6/V5-His) T7/pEF-5 Pcdna3.1R/BGH rev pEF1(4;6/His_A;_B;_C) T7/Xpressfor Pcdna3.1R/BGH rev pEF5/FRT/V5-D(-TOPO) T7/pEF-5 Pcdna3.1R/BGH rev pEGFP M13rev EGFP-NrevpEGFP-1 not available EGFP-Nrev/ PEGFP-N-3′pEGFP-C PEGFP-C-5′ PEGFP-C-3′pEGFP-C1 EGFP-Cfor SV40-pArevpEGFP-C2 EGFP-Cfor SV40-pArevpEGFP-C3 EGFP-Cfor SV40-pArevpEGFP-F EGFP-Cfor SV40-pArevpEGFP-N PEGFP-N-5′ PEGFP-N-3′pEGFP-N1 CMV-Profor EGFP-NrevpEGFP-N2 CMV-Profor EGFP-NrevpEGFP-N3 CMV-Profor EGFP-NrevpENTR M13F M13R pET-11(-a,-b,-c,-d) T7 T7-TerminatorpET-12(-a,-b,-c) T7 T7-Terminator pET-14b T7 T7-Terminator pET-15b T7 T7-TerminatorpET-16b T7 T7-TerminatorpET-17b T7 T7-TerminatorpET-17xb T7 T7-TerminatorpET-19b T7 T7-TerminatorpET-20b(+) T7 T7-TerminatorpET-21(-a,-b,-c,-d)(+) T7 T7-Terminator pET-22b(+) T7 T7-TerminatorpET-23(-a,-b,-c,-d)(+) T7 T7-TerminatorpET-24(-a,-b,-c,-d)(+) T7 T7-Terminator pET-25b(+) T7 T7-TerminatorpET-26b(+) T7 T7-TerminatorpET-27b(+) T7 T7-Terminator pET-28a(-b,-c)(+) T7 T7-TerminatorpET-29a(-b,-c)(+) S.tag/T7 T7-TerminatorpET-3(-a,-b,-c,-d) T7 T7-Terminator pET-30_Ek/LIC T7 T7-Terminator pET-30_Xa/LIC T7 T7-T erminator pET-30a(-b,-c)(+) S.tag/T7 T7-Terminator pET-31b(+) not available T7-T erminator pET-32_Ek/LIC TRXfor T7-T erminator pET-32_XA/LIC TRXfor T7-Terminator pET-32a(-b,-c)(+) S.tag/TRXfor T7-Terminator pET-33b(+) T7 T7-Terminator pET-34b(+) S.tag T7-Terminator pET-35b(+) S.tag T7-T erminator pET-36b(+) S.tag T7-Terminator pET-37b(+) S.tag T7-Terminator pET-38b(+) not available T7-Terminator pET-39b(+) S.tag T7-T erminator pET-40b(+) S.tag T7-Terminator pET-41_Ek/LIC GST-Cfor T7-Terminator pET-41a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator pET-42a(-b,-c)(+) S.tag/pGEX-5 T7-Terminator pET-43.1a(-b,-c)(+) S.tag coliDOWN pET-43.1a_EK/LIC S.tag not available pET-44a(-b,-c)(+) S.tag T7-Terminator pET-44a_EK/LIC S.tag not available pET-45b(+) T7 T7-Terminator pET-46_Ek/LIC T7 T7-Terminator pET-5(-a,-b,-c) T7 T7-Terminator pET-52b(+) T7 T7-Terminator pET-7 T7 T7-Terminator pET-9(-a,-b,-c,-d) T7 T7-T erminator pET coco-1 T7 T7-Terminator pETDuet-1(MCSI) pETUP1 DuetDOWN1 pETDuet-1(MCSII) DuetUp2 T7-Terminator pET-T4 T7 T7-Terminator pEYFP-C1 pEGFP-C-5’ pEGFP-C-3' pEYFP-N1 pEGFP-N-5’,CMV-Profor pEGFP-N-3’ pFLAG-ATS N26for pTrcHis-rv；C24rev pFLAG-CTC N26for pTrcHis-rv；C24rev pFLAG1 N26for pTrcHis-rv；C24rev pFLAG-CMV(-2) CMV-30；CMV-Profor CMV-24 pFLAG-CMV1 CMV-30 CMV-24pGAD424 GAL4_ADfor 3'ADpGADGH GAL4_ADfor 3'ADpGADT7 T7/GAL4_ADfor 3'AD pGADT7-Rec T7/GAL4_ADfor 3'AD pGADT-T T7 3,BD pGAD10 GAL4_ADfor 3'AD pGAPZa-A a-FACTOR 3'AOX pGBKT7 T7 3,BDpGBT9 MATCHMAKER5'DNA-BD/GAL4BD MATCHMAKER3'DNA-BDpGEM-1 T7 SP6pGEM-3 T7 SP6 pGEM-3Zf(+)(-) SP6/M13rev T7/M13for pGEM-4 T7 SP6pGEM-4Z T7/M13for SP6/M13rev pGEM-5Zf(+)(-) SP6/M13rev T7/M13for pGEM-7Zf(+)(-) SP6/M13rev T7/M13for pGEM-9Zf(+)(-) T7/M13for SP6/M13rev pGEM-T T7/M13for SP6/M13rev pGEM-T_Easy T7/M13for SP6/M13rev pGene/V5-His_A(_B,_C) pGENE-5 BGH rev,V5rev pGEX-2T pGEX-5 pGEX-3 pGEX-2TK pGEX-5 pGEX-3pGEX-3X pGEX-5 pGEX-3 pGEX-4T-1(-2,-3) pGEX-5 pGEX-3 pGEX-5X-1(-2,-3) pGEX-5 pGEX-3 pGEX-6P-1(-2,-3) pGEX-5 pGEX-3 pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2)pGL3-Basic(MCSI) RVP3(PGL3+)? GLP2(PGL3-)?pGL3-Basic(MCSII) RVP4, PLXSN-3'?pGL4pGlow(-TOPO) T7 pGlow-3 pGM-T T7 SP6pGWC SP6 T7pHook-2 T7/CMV-Profor not availablepIND/V5-His_A(_B,_C) not available BGH rev,V5rev PinpointTMVector PinpointPrimer SP6pIRES(MCSI) T7 pIRES-3pIRES(MCSII) not available T3,EBVrevpIRES2-EGFP CMV-Profor,pIRES2-EGFP.P5’ IRESrev,pIRES2-EGFP.P3’ pIRES-hrGFP-2a CMV-Profor,T3 pIRES-hrGFP-3 pIRESneo2 T7/CMV-Profor pIRES-3pIVEX(all) T7/pBRrevBam T7-T erminatorpLEXA pLEXA-F pLEXA-RpLNCX pLNCX-F pLNCX-RpLNCX2 CMV-Profor pRevTRE-3 pLVTHM（H1启动子下游）H1primer SP6 pLXSN pLXSN-F pLXSN-RpMALc MalE primer M13F(-47)pMAL-c2E MalE primer M13F(-47)pMAL-C2x Pmal-c2x-F(P5’) Pmal-C2X-R(P3’ )pMALd MalE primer M13F(-47)pMALe MalE primer M13F(-47)pMAL-P4X MalE primer M13F(-47)pMAL-p2X MalE primer M13F(-47)pMET A/B/C AUG1F AUG1RpMD18-T M13R(-48)ECORI M13F(-47)(HINDⅢ)pMD19-T M13F47 M13R48pMD20-T M13F47 M13R48pMOSBlue T7/M13rev M13forpMSCV-puro pLXSNfw pMSCVrev pMT/V5-His_A(_B,_C) Mtfor BGH rev,V5rev pNTAP T3 T7pCTAP pCTAP5'primer T7pOTB7 M13rev/T7 M13for/SP6 pOptiVEC-TOPO TA CMV-F/CMV-Profor EMCVIRES reverse pPC86 PPC86-F PPC86-R pPC97 PBD-GAL4-F PBD-GAL4-RpPIC3.5K 5'AOX 3'AOXpPic9k(A+,Z+) 5’AOX(Ppic9k-f)/a-factor 3’AOX(Ppi c9k-R) pPICZa 5'AOX/PPICZa-F 3'AOX pPROEXHTA M13R(-48) 无反向引物pProEX-HTa(b,c) M13R48/ Tac-Profor pBADrv/pTrcHis-3 pPD129.36 M13F20/M13F pQE30or40or60(AMP+) PQE30-F PQE-30R pRC/CMV2/CAT CMV/T7pRECEIVER CMV-F 此载体无反向引物pRECEIVER-M03 pRECEIVER-M03F pRECEIVER-M03R pREP4 pREP4 forward primer EBV reverse primerpRK5 SP6 pSG5-3pRSET T7/Xpressfor T7ter pRSFDuet-1(MCSI) DuetUP1 DuetDOWN1 pRSFDuet-1(MCSII) DuetUP2 T7TER pSecTac2 T7/CMV-Profor BGH rev/c-mycrevpSG5 pSG5-5,/T7 PSG5-R(SV40)pSHlox1 T7 SP6 pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSI T7 EBVrev pSilencer1.0-U6 T7 T3 pSilencer2.0-U6 T7 M13for pSilencer2.1-U6neo T7 M13for pSilencer3.1-H1hygro M13F(-47) 3.0REV pSilencer4_1-CMVneo pSilener4.1-5' CMV-Profor pSILENCEV2.0-U6 T7 2.0REV pSIneo T7 EBVrev,T3Psit T7 T7T pSK01-T M13F M13RpSK-CMV(KAN+) T7 T3 pSOS Psos5' Psos3'pSP64 SP6 M13revpSP64/65 SP6 此载体无反向引物pSP65 SP6 M13revpSP70 SP6 T7pSP71 SP6 T7pSP72 SP6 T7pSP73 SP6 T7pSport1 SP6/M13F(-47) T7/M13RpSPORT1 SP6/M13for T7/M13revpSPT18 SP6 T7pSTBlue T7/M13rev SP6/M13for pSUPER.Basic T7/M13F M13R pSUPER T7 T3pSURE-T M13F M13R pSURE-Tsimple M13F-47 M13R-48 pSuperCos pBRforEco T7pT3T7 M13rev/T3 M13for/T7pT7-7 T7 not availablepT7Blue? T7 M13F(-47)pT7T3_18U M13rev/T7 M13for/T3 pT7T3D-PAC M13rev/T7 M13for/T3 pTA2 M13F/T7(优先使用M13F）T3/M13R pT-Adv M13rev T7,M13forpTAg T7/M13rev M13for pTARGETTM pTarget.F(在T7前面）/T7 pTarget.R pTG19-T M13F(-47) M13R(-48) pTARGETTM T7/M13F PTARGET-SEQ pThioHisA,B,andC Trx Forward Trx Reverse / M13forpTO-T7 T7 T7TERpTRC99A PTRC99A-F pTrcHis-rvpTrc99a-c PTRC99C-F PTRC99C-RpTRC99a-c PTRC99C-F PTRC99C-RpTrcHisB PTrcHisA-F/Xpressfor PTrcHisA-RpTrcHisA PTrcHisA-F PTrcHisA-RpT-REX-DEST30 TREfor T7 pTriEx1 T7/TriExup TriExdownpTriEx1.1 T7/TriExup TriExdownpTriEx1.1_Hygro T7/TriExup not availablepTriEx1.1_Neo T7/TriExup IRESrevpTriplEX1 TriplEx-LD-5 T7,TriplEx-LD-3pTriplEX2 TriplEx-LD-5；pTR5‘ T7,TriplEx-LD-3pTWIN-1 T7 T7TERpTXB1 T7 MxeInteinrevpTXB2 T7 MxeInteinrevpTXB3 T7 MxeInteinrevpTYB1 T7 InteinrevpTYB2 T7 InteinrevpTYB3 T7 InteinrevpTYB4 T7 InteinrevpTZ_18U T7/M13rev M13forpUB6/V5-His UB-F BGH rev,V5rev pUC13 M13rev/M13rev(-48) M13for/M13for(-49)pUC18 M13rev/M13rev(-48) M13for/M13for(-49) pUC18(19)/118(119) M13F(-47) M13R(-48)/PUC19(HINDⅢ) pUC19 M13rev/M13rev(-48) M13for/M13for(-49)pUC57 M13rev/M13rev(-48) M13for/M13for(-49)pUC8 M13rev/M13rev(-48) M13for/M13for(-49)pUC9 M13rev/M13rev(-48) M13for/M13for(-49)pUM-T M13F(-20) M13R(-20)pUCm-T(AMP+) M13F/T7 M13RpUNI/V5-His not available BGH rev,V5revpVAX1T7 T7/CMV-Profor PCDNA3.1R/BGHR pVP22/mycHis VP22-Cfor BGH rev,c-mycrev pVP22/mycHis2 T7/CMV-Profor VP22-Nrev pWE15 pBRrevHind T7pYES2 T7 pYes2.R pYES-DEST52 T7/GAL1-Profor V5rev/CYC1-Terminator pYEX_4T_1 Clontech pYEX_4T-3 pYEX_4T_2 pGEX-5 pYEX_4T-4pYEX_4T_3 pGEX-5 pYEX_4T-4pYEX_4T_4 pGEX-5 pYEX_4T-4 pZeoSV2+/hu-Tyr T7 not available pZERO-1 M13rev/SP6 T7/M13forpZERO-2 M13rev/SP6 M13for/T7pZome-1-C Zome-F Zome-RpZome-1-N Zome-F Zome-R YCp50(BamHI-site) PBRforBam PBRrevBam YEp13(BamHI-site) PBRforBam PBRrevBam YEp24(BamHI-site) PBRforBam PBRrevBam YIp5(BamHI-site) PBRforBam PBRrevBam RNAi-ReadypSIREN-RetroQ-U6Zs Green U6菌PCDB T7 BGH 天为时代TA载体T7 T3 m13（+）T7？T3？pMSCV-neo pMSCV-5' pMSCV-3'。