5 Viruses can be used as vectors for whole plants
6 Guns and electric shocks transfer DNA into plant cells
7 Molecular cloning of plant genes
8 Arabidopsis is being used as a model organism for molecular genetic analysis of plants
Ⅳ Application of plant genetic engineering
Ⅴ Biosafety
Genetic Engineering of Plants
Ⅰ Plants are very important for mankind
1) Photosynthesis 2) Food 3) Environmental improvements
Genetic Engineering of Plants
Ⅲ Principles and methods
1 Whole plants can be grown from singe cells
2 Leaf disks are an important target for gene transfபைடு நூலகம்r
4 Reporter genes demonstrate transgene expression in plant tissues
5 Viruses can be used as vectors for whole plants
T-DNA contains: 1) opine systhesis genes 2) iaaM, iaaH—auxins 3) iptZ--phytohormone
Genetic Engineering of Plants
Ⅰ Plants are very important for mankind
ⅡMain goal of plant genetic engineering
is making transgenic plants
Fundamental elements 1) specific genes 2) vectors 3) regeneration of plant
3 Ti plasmid of Agrobacterium 4 Reporter genes demonstrate
transgene expression in plant tissues 5 Viruses can be used as vectors for
whole plants
Genetic Engineering of Plants
crown gall tumors 2)T-DNA, part of the Ti plasmid, is
transferred to plant cells
3)T-DNA has been modified to act as a gene vector
4 Reporter genes demonstrate transgene expression in plant tissues
5 Viruses can be used as vectors for whole plants
Ⅲ Principles and methods
3 Ti plasmid of Agrobacterium 1)Ti plasmid of Agrobacterium causes
crown gall tumors 2)T-DNA, part of the Ti plasmid, is
Genetic Engineering of Plants
Ⅰ Plants are very important for mankind
Ⅱ Main goal of plant genetic engineering is making transgenic plants
Ⅲ Principles and methods
1 Whole plants can be grown from singe cells
2 Leaf disks are an important target for gene transfer
3 Ti plasmid of Agrobacterium
4 Reporter genes demonstrate transgene expression in plant tissues
Genetic Engineering of Plants
Ⅰ Plants are very important for mankind
ⅡMain goal of plant genetic engineering
is making transgenic plants
Ⅲ Principles and methods
Ⅲ Principles and methods
1 Whole plants can be grown from singe cells
2 Leaf disks are an important target for gene transfer
3 Ti plasmid of Agrobacterium 1)Ti plasmid of Agrobacterium causes crown gall tumors 2)T-DNA, part of the Ti plasmid, is transferred to plant cells 3)T-DNA has been modified to act as a gene vector
Ti plasmid---circular ds DNA about 200kb
Genetic Engineering of Plants
Ⅲ Principles and methods
3 Ti plasmid of Agrobacterium 1)Ti plasmid of Agrobacterium causes