姓名班级学号实验日期2014.5.21科目实验名称Mouse Dissection合作者指导教师成绩LAB 10: Mouse DissectionIntroduction:In biomedical research, animal models are always regarded as indispensable tools. They contribute to the scientific discovery in biology and our understanding of the functions of individual genes, even the mechanism of different diseases. Typically, although mice are different from humankind in size and appearance, they have a distinct genetic similarity. At the same time, mice have an efficient ability to reproduce, so they are important research tools for experiments in the lab.In this experiment, we will exercise to dissect a mouse, so that we can observe the inside of a mammalian body to identify the female and male mice. Learning and recognizing the anatomical structure of mice, including.Materials and Methods:Materials:Operation plate, Scissor, Forceps, Alcohol cotton, Mouse.Methods: [we get a male mouse]Part 1: Observations of external features. Make a table T-1.1．Having an overhead view, identify the mouse’s head, neck, truck, and tail; observe the dorsal and ventral surfaces. Roughly record what is seen.2．Observe the thorax which is supported by the rib cage, and the abdomen, and the details of appendages attached to the abdomen.3．Find the mouth, two external nostrils, two external auditory canals, and anus, which are on the body surface.4．Have a simply look at the surface of reproductive organ, prepuce, urethral and penis including.And locate the saclike scrotum; feel for the paired testes in the scrotum. Note the roughfeatures.Part 2: Observation of organs and structures inside the mouse. [Open the Ventral Body Cavities, Thoracic Cavity, and Abdominopelvic Cavity in order. We must break the ribs near the attachment to the vertebral column to fold back the upper flaps.] Make a table T-2.1.Ventral Body Cavities.a. Make a longitudinal incision through the skin with a dissecting scissor, from the neck to thepreputial opening. Pierce the body wall below the ribs, with the blades angled upward, sothat it won’t damage the internal organs.b. Pull the sides of the longitudinal incision, and look for the diaphragm. Then make two2. Thoracic Cavity.a. After opening, observe the heart and cut the thymus away so that we can observe the heartclearly.b. Make sure the location of lung, find the trachea and bronchus. Note the features in table T-2.3.Abdominopelvic Cavity.a.Observe the liver which is posterior to the diaphragm, look for the gallbladder.b.Locate the stomach, and then examine the spleen. Note where the small intestine is, and lookfor the pancreas (can secrete insulin). Take photos and record the features.c.Find the place where small intestine empties into large intestine. Observe the short rectumwhich is near to anus, and cecum.d.Separate the esophagus, dorsal mesentery, and the descending portion of the large intestine.Give the urinary bladder.e.Find two kidneys, and the urethra connected to them, the ureter start from the central ofkidney.f.Examine the organs of the reproductive system. Use scissors to open the scrotum to exposethe testis, and then find the coiled epididymis and vas deferens.g.Roughly observe a mouse of the opposite sex.Results:Part 1: table T-1As we can see, the blood vessels on the ear are apparent, and the penis is wrapped in prepuce. The saclikePart 2: table T-2The bronchi can carry air from the trachea toward the lungs, during the process of inspiration.The small intestine is longest organ in the body, filling most of the Abdominopelvic cavity.Pancreas produces enzymes for the digestion of food and, as a part of the endocrine system, it secretes insulin into the blood.The system of female mouse is similar to male we observe, except the reproductive organs. Discussions:Question 1: How to distinguish female and male mouse?From the appearance of external genital organs, we can recognize them, the difference of penisand vagina mouth is apparent. Besides, the female mouse has many visible nipples and theRespiratory system: lungs, trachea, bronchus.Digestive system: stomach, spleen, pancreas, liver, gallbladder. Urinary system: kidney, ureter, urinary bladder.Reproductive system: penis, prepuce, saclike scrotum.References:/article/495ba8413be36138b30ede13.html/link?url=0rmCCGLBKZt3--XtPeTfAzxlE3TKwCkGpt9pNOCfExKwE_xen4UsnC PSEESJL YLa-uCgEjRaB17EuNGatXZr3q/link?url=ijiTYru5lEYl1NIVmt_oa8ahhUc5NMksycmM_FukfG80Fn7N6yt1GlD00RXaz4aBJIUH8YSM0sbyr_NWszwF3a/link?url=6e5jeclQ_pBDBEadBRpQrOXzQOTKXPP_fVYZ1UaYWVSVGsUtQg0BoDWUQ3hg_kHXnWmkmBYq0xf7M10V9Mnx5aContribution statements:%%% is responsible for recording the phenomena, when %%% operates the mouse, and %%% prepares the materials needed.Figures:TailFigure 1-1 Figure 1-2The view of the ventral surface the view of the dorsal surface (belly) (back) MouthNeck Saclike scrotumThorax HeadEarFootAnusFigure 2-1 Figure 2-2 Figure 2-3 The view of mouse after being The mouse after being open Some organs cut through the skin. the ventral body cavity.Heart Stomach Trachea.Figure 2-4 Figure 2-4 Figure 2-5The view of Thoracic Cavity. The inside view of abdomen. The inside view of thorax.Figure 2-6. Figure 2-7Intestines. Some organs of digestive system. DiaphragmLung,4 pieces.ThymusLarge Intestine CecumSmallintestine Pancreas.Spleen.Penis. KidneyUrinary bladderFigure 2-8 Figure 2-9 The view of inside reproductive organ.Figure 2-10 Figure 2-11 Figure 2-12 The gallbladder The adrenal gland. The ureter. Vas deferens.Epididymis第页。