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Microarray_Printing基因微阵列打印


Microarray Printing 基因微阵列打印
陈光磊
厦门大学模式识别与智能系统研究所
Play the video microarryer.flv
High Through-put Printer for Whole Genome Chips 图片出处:/people/macampbell/CSU/Introscanners.html
In Action

1:00
perfusion pump for the wash well

1:25 清洗池的灌注泵 1:45 又在同一组玻片上点了一次

注意到视频右下角的英文缩写了吗? HHMI(HOWARD HUGHES MEDICAL INSTITUTE霍华德·休斯医学研究会) 成立于1953年,是一家为全美科学家提供资助的富有卓越声望的非盈 利型研究机构. HHMI, a non-profit medical research organization that ranks as oneof the nation's largest philanthropies, plays a powerful role inadvancing biomedical research and science education in the U.S. Inthe past two decades HHMI has made investments of more than $8.3billion for the support, training, and education of the nation'smost creative and promising scientists. The Institute commitsalmost $700 million a year for research and distributes more than$80 million in grant support for science education. 更多请见: AVideo Introduction to HHMI /about/hr_movie/
384-well plate with small molecules
384池(小凹槽)渡上小分子
8X48=384
图片出处:/productDetails.asp?prodID=130 /catalog.asp?section=product&groupID=52
图片出处: /Products/B uffers/MCS/mcs.html

Drying Chamber

时间:00:41 干燥室 相当于干燥剂,烘干机。或者下面的设备
Washing Chamber

时间00:50 清洗室 00:54洗完又干燥了一次

Sonicator工作原理
Micro Cleaning Solution at 1X concentration reduces surface tension and enhances cavitation(空洞现象) for superior microarray pin cleaning at the molecular(分子) and atomic (原子) levels. Acoustic waves (声波,箭头所示) formed by a sonic transducer(传感器) produce cavitation bubbles (red spheres) that deliver the cleaning force to pins and other types of microarray hardware.
Sonicator for Cleaning

Sonicator 4000 是世界上最先进的超声波破碎仪。 应用范围:
1.细胞破碎/裂解 2.抽提蛋白、核酸 3.修剪DNA/RNA 4.纳米技术研究 5.染色质免疫沉淀技术 6.样品均质、乳化 7.其它样品处理 (眼睛店帮你洗眼镜也用到) 时间00:33
48-pin printhead



定位00:29 针头排列(4X12)与我们获得图像的区块分布一样 一个针点一个22X22的斑点区?
A closeup of a microarray printer head, showing the detail of a microarray printing pin. /programs/as k/082406.html 图片出处: /stealth4bmicro spottingpinbubble145umfeatureswith06 ulbubbleuptake.aspx
Others


另一个牌子的点样机Array it arrayit pointer.flv 无字幕有解说 slow printing.flv Microarray Printing
Printing microarrays at the UHN Microarray Centre (这个针头的速度慢了点, 但可以从近距离观察其工作细节。可惜没有文字说明)
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