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教研部细胞库常用细胞株(细胞及资料来源食品工业发展研究所生.

Biosafety level:2
HepG2
60025
human
hepatoblastoma
90%-MEM
10%FBS
HL60
60027
human
promyelocytic leukemia
80%Iscove’s Modified Dulbecco’s
Media(IMDM)
20%FBS
生長特性:suspension
Species
Tissue
medium
note
TOV-112D
60408
human
ovarian cancer
85%1:1 MCDB 105 and Medium 199
Cell line
BCRC No.
Species
Tissue
medium
note
MCF7
60436
human
breast adenocarcinoma
90%-MEM
10%FBS
細胞成團生長且易漂浮。解凍初期,部份細胞呈懸浮性cluster,貼附的細胞,部分是epithelial-like,部分為圓形。待第2-3次繼代培養後細胞逐漸生長成扁平狀之epithelial-like細胞。
RL95-2
60103
human
endometrial carcinoma
90%1:1 ratio mixture of DMEM and
Ham’sF12 medium
10%FBS
SW480
60249
human
colon adenocarcinoma
90%Leibovitz’s L15 medium
10%FBS
培養條件:
without CO2in air
T24
60062
human
bladder carcinoma
90%McCoy’s5a medium
10%FBS
(續)教研部細胞庫常用細胞株(細胞及資料來源:食品工業發展研究所生物資源保存及研究中心(BCRC))第4頁,共4頁
Cell line
BCRC No.
LNCaP cloneFGC
60088
Human
prostate carcinoma
90%RPMI 1640 medium
10%FBS
解凍或繼代後,靜置48小時,勿移動,細胞才會貼附良好。
(續)教研部細胞庫常用細胞株(細胞及資料來源:食品工業發展研究所生物資源保存及研究中心(BCRC))第3頁,共4頁
ES-2
60067
human
ovarian carcinoma
90%McCoy’s 5a medium
10%FBS
HCT 116
60349
human
colorectal carcinoma
90%McCoy’s5a medium
10%FBS
To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37℃to facilitate dispersal.
◎教研部細胞庫常用細胞株(細胞及資料來源:食品工業發展研究所生物資源保存及研究中心(BCRC))第1頁,共4頁
Cell line
BCRC No.
Species
Tissue
medium
note
293
60019
human
embryonic kidney
90%-MEM
10%FBS
Biosafety level:2
Media(IMDM)
10%FBS
生長特性:suspension
KATO III
60200
human
gastric carcinoma
80%Iscove’s Modified Dulbecco’s
Media(IMDM)
20%FBS
生長特性:mixed ; adherent & suspension
細胞生長較慢。繼代培養時,吸附的細胞請用細胞刮刀刮下。
90%RPMI 1640 medium
10%FBS
Biosafety level:2
細胞含Integrated HPV-16 genome,操作培養時應注意HPV感染。
COLO205
60054
human
colon adenocarcinoma
90%RPMI 1640 medium
10%FBS(heat-inactivated)
MDA-MB-231
60425
human
breast adenocarcinoma
90%Leibovitz’s L15 medium
10%FBS
培養條件:
without CO2in air
NIH/3T3
60008
mouse
NIH/Swiss embryo
90%Dulbecco’s modified Eagle’s
(續)教研部細胞庫常用細胞株(細胞及資料來源:食品工業發展研究所生物資源保存及研究中心(BCRC))第2頁,共4頁
Cell line
BCRC No.
Species
Tissue
medium
note
HeLa
60005
human
cervical epithelioid carcinoma
90%-MEM
10%FBS
解凍時建議離心去除DMSO,且初期生長較慢約需一星期培養。
IMR-32
60014
human
neuroblastoma
90%-MEM
10%FBS
細胞易脫落,繼代培養時直接pipetting即可。
K-562
60007
human
chronic myelogenous leukemia
90%Iscove’s Modified Dulbecco’s
生長較慢,且放置室溫時會detached。
A549
60074
human
lung carcinoma
90%Ham’s F-12Kmedium
10%FBS
AGS
60102
human
gastric carcinoma
90%Ham’s F-12Kmedium
10%FBS
CaSki
60251
human
cervical epidermoid carcinoma
medium(DMEM)
10%calf serum
contact-inhibited cell,不要培養至completely confluency。
Raji
60116
human
Burkitt's lymphoma
90%RPMI medium
10%FBS
Biosafety level:2
生長特性:suspension
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