Commonly used strainsThis page describes some of the most commonly used yeast lab strains. Much of the information is taken from F. Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include:
Mortimer and Johnston (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C
van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly
used lab strainsWinzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic
identity between common lab strainsS288C
Genotype: MATα SUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically.
The S288C genome was recently resequenced at the Sanger Institute.References: Mortimer and Johnston (1986) Genetics 113:35-43.Sources: ATCC:204508BY4743
Genotype: MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0Notes: Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of MIP1 which increases petite frequency. See Brachmann et al. reference for details.
References: Brachmann et al. (1998) Yeast 14:115-32.Sources: Biosystems:YSC1050FY4
Genotype: MATaNotes: Derived from S288C.References: Winston et al. (1995) Yeast 11:53-55.Brachmann et al. (1998) Yeast 14:115-32.FY1679Genotype: MATa/α ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GALNotes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References: Winston et al. (1995) Yeast 11:53-55.Sources: EUROSCARF:10000DAB972
Genotype: MATα X2180-1B trp10 [rho 0]Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.
References: Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.Sources: ATCC:204511A364A
Genotype: MATa ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIONotes: Used in the systematic sequencing project, the sequence stored in SGD.References: Hartwell (1967) J. Bacteriol. 93:1662-1670.Sources: ATCC:208526XJ24-24a
Genotype: MATa ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2Notes: Derived from, but not isogenic to, S288CReferences: Strathern et al. (1979) Cell 18:309-319DC5
Genotype: MATa leu2-3,112 his3-11,15 can1-11Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References: Broach et al. (1979) Gene 8:121-133X2180-1A
Genotype: MATa SUC2 mal mel gal2 CUP1Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180
References: Mortimer and JohnstonSources: ATCC:204504YNN216Genotype: MATa/α ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre
Notes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.YPH499
Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note thattrp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker.gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.
Sources: ATCC:204679YPH500
Genotype: MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1Notes:MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.