( Engineering Research Center of Industrial Microbiology，Ministry of Education，Fujian Normal University，Fuzhou 350108，China)
Abstract: Objective: To obtain high and well defined protease － producing strains． These strains can be used in seafood process to produce poly － peptide． Method: The protease － producing strains were first screen from soil by well － designed sampling sites and colony screening，fermentation supernatant identification and Folin － phenol measurement assay etc． To improve the production of protease capability for the target strain，UV mutagenesis were further introduced． Result: 187 protease － production strains were screened from the collected 12 soil samples． Further secondary screening indicated a strain W9 produced stably higher enzyme activity than any other strains among the primary screened 11 strains． The activity of strain W9 reached to 1 594U / ml． Mutant strain W9UV was obtained by UV mutagenesis which enhances the protease activity by 15． 6% in comparison with that of the wild strain W9，with the activity of 1845 U / ml． Conclusion: W9UV strain has a greater protease activity and more stably fermentation performance． Key words: screen; seafood process; neutral protease; enzyme activity; UV mutagenesis
中图分类号: Q503; Q959． 17 文献标识码: A doi: 10． 3969 / j． issn． 1004 － 311X． 2011． 01． 026
Screening and UV Mutagenesis of Protease Producing Strains
CAI Wan － ling，TIAN Bao － yu* ，GUO Jing，LAN Can － hua，HUANG Wei，HUANG Jian － zhong*
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