Solid product Liquid product Formulation Sterile ultrafiltration Polishing ultrafiltration Formulation Liquids or solids Drying Rotary filter Kerosene and tertiary amine Water Activated carbon CaCl and polyelectrolyte
4. The Objective of the Cour se
(1) Master ing main separ ation oper ations (2) Pr ocess design : developing a engineer ing solution to a given pr oblem under multidisciplinar y envir onment (3) Appar atus design : Scaling up/down or innovation, and Pr oduct design (4) Captur ing the science unity under lying diver sified separ ation oper ations
2. Characteristics of Bioseparations
2. Characteristics of Bioseparations
1. What is Bioseparations? 2. Characteristics of Bioseparations 3. General industrial bioseparation processes 4. The Objective of the Course 5. Content of this course
3. General industrial bioseparatindustrial bioseparation processes
Downstr eam pr ocesses for pr oduction of antibiotic
Pr oduction of r ecombinant human alpha-1-antitr ypsin (
T=5 Bioreactor fluid with cells Filtration fitrate Extraction pH2-3 Back extraction adsorption Spent solvent
Product
Precipitation B-Chain Dialysis
Drying centrifugation Crystallization Filtration Ion exchange
Precipitation A-Chain
Sulfonation
Precipitation
Extraction Downstr eam pr ocessing for pr oduction of liquid or solid pr otease
Recover y and pur ification of human insulin
2001
Homework
Fr iday, 3:00~5:00PM Room B102 or B105, Chemical engineer ing Building
A detailed summary ( 1000-2000 words) 1000of today's topic and any advice today' about the course.
(1) Characteristics of biochemicals or bioproducts
Tremendous variety of products ( )
) )
Biological (or chemical) activity ( low concentr ation of the tar gets (
What is the value of the product? ( ( ) ) What is an acceptable product quality? Where is the product and impurities in each process stream? ( )
What are the unusual physicochemical
Chromatography
Cool to 4-5 Filter aid Bioreactor fluid pretreatment Chromatography Cells Filtration or centrifugation Two-stage ultrafiltration precoat Ultrafiltration
Downstr eam pr ocessing of citr ic acid
3. General industrial bioseparation processes
3. General industrial bioseparation processes
Unit oper ations above pr ocesses include: Filtr ation Centr ifugation Adsor ption Solvent extr action Chr omatogr aphy Ion exchange Dr ying Cr ystallization and pr ecipitation etc.
Fer mentation br oth being a complicated mixtur e (complex components) (
Poor stability (
) )
How to lower them?
Important!
The recovery and the purification processes must be well conceived and well designed!
3. General industrial bioseparation processes
(1) Removal of insolubles. Filtration, centrifugation (2) Isolation of products Adsorption, solvent extraction (3) Purification Chromatography, electrophoresis, precipitation (4) Polishing Crystallization, drying, etc.
properties of the product and the principal impurities?
( )
What are the economics of various alternative separations?
( )
Careful consideration of these questions can help us to determine the optimal separating processes.
References
1. Biosepar ations Science and Engineer ing, Roger G. Har r ison, et. al , Oxfor d Univer sity Pr ess, Inc., New Yor k Oxfor d, 2003 2. 2005
6. References 7. Question answering
(2) Characteristics of Bioseparations
Broad spectrum of separation methods most bioseparation methods come from separations of chemicals These separations are elaborate and expensive
)
3. General industrial bioseparation processes
3. General industrial bioseparation processes
3. General industrial bioseparation processes
Bioreactor fluid with cells Cell disruption Cell debris Centrifugal extraction supernatant Dialysis Cleaning pro insulin
High pur ity and str ict sanitation cr iter ion
2. Characteristics of Bioseparations 2. Characteristics of Bioseparations
So, Before designing, the questions we should ask and answer are:
5. Content of this course
Chapter 1 Intr oduction Chapter 2 Cell disr uption Chapter 3 Flocculation & Sedimentation Chapter 4 Filtr ation & Membr ane Separ ations Chapter 5 Extr action Chapter 6 Adsor ption & Chr omatogr aphy Chapter 7 Cr ystallization and Pr ecipitation Chapter 8 New separ ation methods