(文档含英文原文和中文翻译)中英文对照外文翻译文献Biological effects of the Magnetic Stimulation on the T oad Heart Abstract-W e stimulated the exposed toad heart by a low frequency and high energy magnetic. By analyze the data of this experiment, it shows that the pulsating of the weak toad heart would make change after stimulated by magnetic. W eak heartbeat strengthened, the single peak curve would become the two peaks curve with atria wave and ventricle wave after the magnetic stimulation. But the cycling of rhythmic pulsatile curve of toad doesn't change.I. INTRODUTIONAll life forms have magnetism. All kinds of magnetic field would have some effects on the configuration and activities of life forms that whichever environmental magnetic, additional magnetic or inside magnetic of organism. The biologic effects are related to the characteristics and附录Ⅳ英文文献及翻译the intension of the magnetic field, as well as the species and the tissues of the life forms.The experimentation showed that magnetism stimulation in some range would control the growth of rat tumour, whatever they are in or out the body. Much more they can induce the cancer cells dead.30mT magnetic stimulation would increase the content of NO in the liver and the kidney.Magnetic also can improve the activity of some enzyme and promote the regeneration of nerve tissue.Cell would increase, the bones would be concrescence, the scar would be rehabilitate.The blood rheology and blood cell number both of human and rat would change obviously, DI the blood mucosity would be low.Heart is the most important apparatus of life. It pulsates day and night. Heart once stop pulsating, the life for danger.Numerous scholar pays attention to the role of magnetic field.But they just studied the effects of magnetic stimulation of the heart pacemaker. The experiments about direct effects of stimulate heart by magnetic is very few.The toads are our experiment animals.W e stimulated and noted by the magnetic stimulation equipment and the noted equipment of pulsatile curve made by ourselves. Analyze the results.II. STUFFA.Experiment equipments:①magnetic stimulation equipment; (magnetic intension 8-10T, impulse width 150ms,maximal stimulation frequency 5Hz);②software of noted pulsatile curve (made by ourselves);③cardiomuscular transducer;④Ringer.Sol ;⑤Batrachia instruments; ⑥clip of frog heart; ⑦cotton thread; ®burette.B.Experiment animals: toads.III. METHODA. Destroy the brain and the spinal cord of the toad by stylet:Penetrate into the occipital aperture upright with stylet,destroyed the brain upwards, take back the stylet and destroy the spinal downwards. If the limb of toad were relaxed, it showed that the brain and spinal were destroyed completely.B. Expose the toad heart: Make the toad lying on its back on the winding center. The magnetic aspect is upright through the toad heart.Cut the ventral skin of toad, snip the breastbone,expose the rat heart. Nip the heart tip by clip carefully. Make the cotton thread tied with the clip of frog hear the linked with the cardiomuscular transducer. Do not make the toad heart leave thorax, or it would disturb the experiment results.C.Noted the result:Connect the cardiomuscular transducer with the computer. Take notes the curve of toad heart without giving the stimulate of magnetic fieldD. After three minutes, noted the weak pulsatile curve.E . Make the magnetic intension 10T, electricize 10s.Stimulate the toad heart and record the pulsatile curve.IV. RESUL TSThe abscissa of cardiac rhythmic pulsatile curve is time, the ordinate is constriction power. Take notes for the pulsatile curve of toad heart that exposed just.W e can know the rhythmic pulsatile cycle of the toad heart is 1.5s from fig 1 which show the cardiac rhythmic pulsatile curve of the toad which was exposed the heart just now. There are two waves in each cycle, one is atria wave, the other is ventricle wave. The atria wave is 0.5s and the ventricle wave is 1.0s. The constriction power of atria is less than that of ventricle. The amplitude of constriction power of ventricle is the 2 times of the atria.Fig. 4.1. It is rhythmic pulsatile curve of the toad without magnetic stimulation.The constriction power of toad heart would become weaker after the toad heart was exposed for a while. At the same time,atrium wave and ventricle wave can not be already distinguished. Heart contracting amplitude were reduced obviously, do not go to the half of original atrium wave. The rhythmic pulsatile cycle of the toad heart is still 1.5s.Fig. 4.2. It is the weak pulsatile curve of toad without magnetic stimulation.But we can distinguish the atria wave and the ventricle wave again after giving the toad heart a magnetic stimulation on following picture. And the amplitude of ventricle waves is more than that of the single wave. The rhythmic pulsatile cycle of the toad heart is still 1.5s.There were six toads as experiment animal in our experiment.After exposing heart a time, the rhythmic pulsatile curve all became single peak curve. Stimulate them when the single amplitudewas 0.95. Noted the data and analyze them.Following is the pulsatile curve of the six toads recorded which were stimulated by magnetic field.Fig. 4.3. It is the pulsatile curve of the fist toad which heart was stimulated by magnetic field.Fig. 4.4. It is the pulsatile curve of the second toad which heart was stimulated by magnetic field.Fig. 4.5. It is the pulsatile curve of the third toad which heart was stimulated by magnetic field.Fig. 4.6. It is the pulsatile curve of the fourth toad which heart was stimulated by magnetic field.Fig. 4.7. It is the pulsatile curve of the fifth toad which heart was stimulated by magnetic field.Fig. 4.8. It is the pulsatile curve of the six toads which heart was stimulated by magnetic field.V. COMPARISIIONRecord ventricle wave amplitude and atrium wave amplitude of the six toads after magnetic stimulation.T able. 5.1. From "T oad1"to "T oad6" expressed the six toads which was stimulated by magnetic field. The "T oad0" expressed the toad which was not stimulated by magnetic field. "T oad7" expressed the toad which pulsated weakly.amplitudes of atria wave amplitudes of ventricle wave T oad0 2.275 2.34T oad1 1.140 1.170T oad2 1.120 1.129T oad3 1.165 1.18T oad4 1.120 1.128T oad5 1.214 1.230T oad6 1.151 1.169T oad7 0.95 Express the toad which was not stimulated by magnetic with"T oad 0", and express the toad which pulsate weakly with"T oad 7". Make histogram to contrast by these data. The first histogram was made by the data of the pulsatile amplitudes of when toad was not gets stimulate and pulsateweakly, as well as the pulsatile amplitude of the fist stimulated toad. After magnetic stimulation, amplitudes of atria wave and ventricle wave were higher than single wave of weak heart. But it is more low than the amplitudes of heart when just exposes obviously.Fig. 5.1. The histogram was make by the amplitudes of the toad exposed heart justly and the toad which stimulated by magnetic field, the toad which pulsate weakly. The "T oad 0" expressed the toad which was not stimulated by magnetic field. The "T oad 1" expressed the fist toad which was stimulated by magnetic field. The "T oad 7" expressed the toad which pulsated weakly.Make histogram respectively with the data of amplitude of each toad stimulated by magnetic field and the amplitude of single wave. Make histogram with the data of amplitudes of six toads stimulated by magnetic field, and compare them.Fig. 5.2. The histogram was made by the amplitudes of the first toad which was stimulated by magnetic field and the toad which pulsate weakly. The"T oad 1" expressed the fist toad which was stimulated by magnetic field. The"T oad 7" expressed the toad which pulsated weakly.Fig. 5.3. The histogram was made by the amplitudes of the second toad which was stimulated by magnetic field and the toad which pulsate weakly The "T oad2" expressed the second toad which was stimulated by magnetic field. The "T oad7" expressed the toad which pulsated weakly.Fig. 5.4. The histogram was made by the amplitudes of the third toad which was stimulated by magnetic field and the toad which pulsate weakly. The"T oad 3" expressed the third toad which was stimulated by magnetic field. The"T oad 7" expressed the toad which pulsated weakly.Fig. 5.5. The histogram was made by the amplitudes of the fourth toad which was stimulated by magnetic field and the toad which pulsate weakly. The "T oad 4" expressed the fourth toad which was stimulated by magnetic field.The "T oad 7" expressed the toad which pulsated weakly.Fig. 5.6. The histogram was made by the amplitudes of the fifth toad which was stimulated by magnetic field and the toad which pulsate weakly. The "T oad5" expressed the fifth toad which was stimulated by magnetic field. The "T oad7" expressed the toad which pulsated weakly.Fig. 5.7. The histogram was made by the amplitudes of the was stimulated by magnetic field and the toad which puls,"T oad 6" expressed the sixth toad which was stimulated by ma "T oad 7" expressed the toad which pulsated weakly.Fig. 5.8. The histogram was made by the amplitudes of the was stimulated by magnetic field.Fig. 5.9. The histogram was made by the amplitudes of the was stimulated by magnetic field and the toad which pulsate weakly.There is discrepancy between the pulsatile a each toad which stimulated by magnetic field. This is dividual discrepancy, it is related with the strong of the experiment animals. But if compared these pulsatile amplitudes of toads which stimulated by magnetic field with amplitude of the toad which pulsated weakly at the same time of discrepancy is very not obvious.VI. CONCLUSIONSThere are a P wave and a QRS wan pare the pulsatile curve with the electrocardiogram to we can discover that the P wave that express atrium constriction is earlier than atria wave.the ORS wave that express ventricle constriction is earlier than ventricle constriction is earlier than ventricle wave. Heart constriction connected closely with the change of biological electricity of cardiac muscle. Before heart contracts,must occur on muscle cell membrane a movement potential that can be conducted, pass through then excited-contract unite can just arouse muscle cell contract to respond. The P wave and QRS wave of electrocardiogram reflect atrium and ventricle respectively with the electrical change in polarization course. Atrium wave and ventricle wave reflect atrium and ventricle respectively the mechanical campaign. Mechanical campaign is only initiated from electrical campaign. So P wave is earlier than atrium wave, QRS wave are earlier than ventricle sixth toad which wave.When the pulsatile rhythmically of heart stopped or in disorder.the electric attack would be helpful on clinic data. The magnetism stimulation may have the same effects as the electric stimulation based on electromagnetism.The pulsatile curve of toad which just exposed heart can divide into atrium wave and ventricle wave. After a time, heart is weak gradually, right now, heart contracts intensity weakens obviously. Atrium wave can not already distinguish with ventricle wave on the curves of toad weak pulsatilecurve Original two summit curves change to single summit curve,and contract range reduces obviously. Do not go to the half of original atrium wave. But heart pulsatile period still ask 1 second. Stimulate toad heart, the direction of magnetic field vertical cross toad heart center from the back to belly. T ake T oad 6 notes at once, the pulsatile curve of toad recovery became original two summit curves. And the amplitude of ventricle six toads which wave worth than single wave is in height of.T ested result proves that the magnetic stimulation of high energy can promote toad heart strength obviously, but for the pulsatile curve period does not be acted on obviously. Can make the curve of pulsatile curve already can not be districted the atrium wave and ventricle of the weak heart recovery that atrium constriction with ventricle constriction alternately.The cell of cardiac muscle has special electrical physiology.Electrical stimulate can affect the electrical physiology moving of heart obviously. Magnetic field and electric field have the characteristic that changes mutually. The role of extra magnetic field can also arouse the ion current in the organism toad 7 cell of cardiac muscle to occur change. Therefore, it changes the electrical physiological campaign of the cell of cardiac muscle, change heart contract condition.Compared with direct electrical stimulation, the magnetic stimulation has a lot of advantages. It shows by clinical information, eliminate the heart shake of human body with current (go through chest wall) to need the energy of 150-350 J probably, directly eliminate heart shake to need the energy of 16-24 J probably. Specific size and the current distribution of electrode have relevant uniformity. The magnetism of biological organization is even basically, magnetic field reaches the deeply layer organization of organism very easily on toad through skin and skeleton. The magnetic stimulation does not have wound. The resistance rate of skin and skeleton is great.Induction current and organization resistance become inverse ratio. There is a small current passes through organism when was stimulated by magnetic field, so person does not have uncomfortable feeling. The body and coil are not contacted in the magnetic stimulation therefore we can stimulate directly without doing any handling for skin in advanced, will not arouse pain.And the body does not have electricity connect with environment, so have very good safety.Just start for the study of biological effects of the magnetic stimulation on life-form.Quantification of the effects of the magnetic stimulation of pulsatile curve still needs to be study furtherACKNOWLEDGMENTThis paper is supported by the National Natural Science Foundation of Chinese (No. 59977024)REFERENCES[1] A.B. Smith, C.D. Jones, and E.F. Roberts, "Article Title", Journal,Publisher, Location, Date,pp. 1-10.[2] Jones, C.D., A.B. Smith, and E.F. Roberts, Book Title, Publisher,Location, Date.[3] Xiao Hongyu, Zhou W anshong, the Development of the Biological Effect of Magnetic Field onHome. Chin .T Phvs Thcr, Fclnvarv. 1999, V ol. 22.[4] Chang Hanyin, BIOMAGNIJTISM, 2003; 3(2): 6.[5] Li Guodong, the Research and Development of Biological Magnetism Application on 2003-2004. BIOMAGNIJTISM, 2004 V ol.4, NO.4:25-26.[6] Y ao T ai, Physiology [M], Beijing: People's sanitary press, 2001. SE57.[7] Guo Fengmei, Zhang Guilian, Cheng Xianghui, Liu Jianling,BIOMAGNIJTISM 2004 V ol. 4,No. 2.[8] Song Shijun, Guo Shumei, W ang Fuwei, the Method that Synchronous Record Machinery andElectricity Activity of Frog Heart. TOLRNAL OF HEBEI MIDICAL UNIVERSITI, VOL. 27, N o. 4 July 2000.高能磁场刺激对蟾蜍心脏搏动影响的生物学研究摘要：我们采用低频高能磁场对蟾蜍的暴露心脏给予刺激，实验结果表明一定强度的磁场刺激可使衰弱的蟾蜍心脏搏动有增强，表现为心肌收缩力度增强,心搏收缩曲线由衰弱时的单波曲线恢复为体现心房收缩和心室收缩的双波曲线，但心脏收缩周期不变。