pAAV-‐CAG-‐GFP编号 载体名称 北京华越洋VECT10004 pAAV-‐CAG-‐GFPpAAV-‐CAG-‐GFP载体基本信息载体名称: pAAV-‐CAG-‐GFP质粒类型: 腺病毒载体；荧光蛋白报告载体高拷贝/低拷贝: 低拷贝克隆方法: 限制性内切酶，多克隆位点启动子: CAG (CMV e nhancer/chicken β-‐actin p romoter) 载体大小: 5439 b p5' 测序引物及序列: pCAG f wd p rimer 5’CAACGTGCTGGTTATTGTG 3’ 3' 测序引物及序列: cggcttctggcgtgtgac载体标签: -‐-‐载体抗性: 氨苄青霉素筛选标记: EGFP克隆菌株: Stbl3 或 NEB S tble宿主细胞（系）: 常规哺乳动物细胞系备注: -‐-‐稳定性: 稳表达组成型/诱导型: 组成型病毒/非病毒: 腺病毒pAAV-‐CAG-‐GFP载体质粒图谱和多克隆位点信息pAAV-‐CAG-‐GFP载体序列:ORIGIN1 C TTCCGCTTC C TCGCTCACT G ACTCGCTGC G CTCGGTCGT T CGGCTGCGG C GAGCGGTAT61 C AGCTCACTC A AAGGCGGTA A TACGGTTAT C CACAGAATC A GGGGATAAC G CAGGAAAGA 121 A CATGTGAGC A AAAGGCCAG C AAAAGGCCA G GAACCGTAA A AAGGCCGCG T TGCTGGCGT 181 T TTTCCATAG G CTCCGCCCC C CTGACGAGC A TCACAAAAA T CGACGCTCA A GTCAGAGGT241 G GCGAAACCC G ACAGGACTA T AAAGATACC A GGCGTTTCC C CCTGGAAGC T CCCTCGTGC 301 G CTCTCCTGT T CCGACCCTG C CGCTTACCG G ATACCTGTC C GCCTTTCTC C CTTCGGGAA361 G CGTGGCGCT T TCTCATAGC T CACGCTGTA G GTATCTCAG T TCGGTGTAG G TCGTTCGCT421 C CAAGCTGGG C TGTGTGCAC G AACCCCCCG T TCAGCCCGA C CGCTGCGCC T TATCCGGTA 481 A CTATCGTCT T GAGTCCAAC C CGGTAAGAC A CGACTTATC G CCACTGGCA G CAGCCACTG 541 G TAACAGGAT T AGCAGAGCG A GGTATGTAG G CGGTGCTAC A GAGTTCTTG A AGTGGTGGC 601 C TAACTACGG C TACACTAGA A GAACAGTAT T TGGTATCTG C GCTCTGCTG A AGCCAGTTA 661 C CTTCGGAAA A AGAGTTGGT A GCTCTTGAT C CGGCAAACA A ACCACCGCT G GTAGCGGTG 721 G TTTTTTTGT T TGCAAGCAG C AGATTACGC G CAGAAAAAA A GGATCTCAA G AAGATCCTT 781 T GATCTTTTC T ACGGGGTCT G ACGCTCAGT G GAACGAAAA C TCACGTTAA G GGATTTTGG 841 T CATGAGATT A TCAAAAAGG A TCTTCACCT A GATCCTTTT A AATTAAAAA T GAAGTTTTA901 A ATCAATCTA A AGTATATAT G AGTAAACTT G GTCTGACAG T TACCAATGC T TAATCAGTG961 A GGCACCTAT C TCAGCGATC T GTCTATTTC G TTCATCCAT A GTTGCCTGA C TCCCCGTCG1021 T GTAGATAAC T ACGATACGG G AGGGCTTAC C ATCTGGCCC C AGTGCTGCA A TGATACCGC 1081 G AGACCCACG C TCACCGGCT C CAGATTTAT C AGCAATAAA C CAGCCAGCC G GAAGGGCCG 1141 A GCGCAGAAG T GGTCCTGCA A CTTTATCCG C CTCCATCCA G TCTATTAAT T GTTGCCGGG1201 A AGCTAGAGT A AGTAGTTCG C CAGTTAATA G TTTGCGCAA C GTTGTTGCC A TTGCTACAG1261 G CATCGTGGT G TCACGCTCG T CGTTTGGTA T GGCTTCATT C AGCTCCGGT T CCCAACGAT1321 C AAGGCGAGT T ACATGATCC C CCATGTTGT G CAAAAAAGC G GTTAGCTCC T TCGGTCCTC1381 C GATCGTTGT C AGAAGTAAG T TGGCCGCAG T GTTATCACT C ATGGTTATG G CAGCACTGC1441 A TAATTCTCT T ACTGTCATG C CATCCGTAA G ATGCTTTTC T GTGACTGGT G AGTACTCAA1501 C CAAGTCATT C TGAGAATAG T GTATGCGGC G ACCGAGTTG C TCTTGCCCG G CGTCAATAC1561 G GGATAATAC C GCGCCACAT A GCAGAACTT T AAAAGTGCT C ATCATTGGA A AACGTTCTT 1621 C GGGGCGAAA A CTCTCAAGG A TCTTACCGC T GTTGAGATC C AGTTCGATG T AACCCACTC 1681 G TGCACCCAA C TGATCTTCA G CATCTTTTA C TTTCACCAG C GTTTCTGGG T GAGCAAAAA1741 C AGGAAGGCA A AATGCCGCA A AAAAGGGAA T AAGGGCGAC A CGGAAATGT T GAATACTCA 1801 T ACTCTTCCT T TTTCAATAT T ATTGAAGCA T TTATCAGGG T TATTGTCTC A TGAGCGGAT1861 A CATATTTGA A TGTATTTAG A AAAATAAAC A AATAGGGGT T CCGCGCACA T TTCCCCGAA1921 A AGTGCCACC T AAATTGTAA G CGTTAATAT T TTGTTAAAA T TCGCGTTAA A TTTTTGTTA1981 A ATCAGCTCA T TTTTTAACC A ATAGGCCGA A ATCGGCAAA A TCCCTTATA A ATCAAAAGA2041 A TAGACCGAG A TAGGGTTGA G TGTTGTTCC A GTTTGGAAC A AGAGTCCAC T ATTAAAGAA 2101 C GTGGACTCC A ACGTCAAAG G GCGAAAAAC C GTCTATCAG G GCGATGGCC C ACTACGTGA 2161 A CCATCACCC T AATCAAGTT T TTTGGGGTC G AGGTGCCGT A AAGCACTAA A TCGGAACCC 2221 T AAAGGGAGC C CCCGATTTA G AGCTTGACG G GGAAAGCCG G CGAACGTGG C GAGAAAGGA 2281 A GGGAAGAAA G CGAAAGGAG C GGGCGCTAG G GCGCTGGCA A GTGTAGCGG T CACGCTGCG 2341 C GTAACCACC A CACCCGCCG C GCTTAATGC G CCGCTACAG G GCGCGTCCC A TTCGCCATT2401 C AGGCTGCGC A ACTGTTGGG A AGGGCGATC G GTGCGGGCC T CTTCGCTAT T ACGCCAGCT 2461 G CGCGCTCGC T CGCTCACTG A GGCCGCCCG G GCAAAGCCC G GGCGTCGGG C GACCTTTGG2521 T CGCCCGGCC T CAGTGAGCG A GCGAGCGCG C AGAGAGGGA G TGGCCAACT C CATCACTAG2581 G GGTTCCTTG T AGTTAATGA T TAACCCGCC A TGCTACTTA T CTACGTAGC C ATGCTCTAG2641 G AAGAGTACC A TTGACGTCA A TAATGACGT A TGTTCCCAT A GTAACGCCA A TAGGGACTT2701 T CCATTGACG T CAATGGGTG G AGTATTTAC G GTAAACTGC C CACTTGGCA G TACATCAAG2761 T GTATCATAT G CCAAGTACG C CCCCTATTG A CGTCAATGA C GGTAAATGG C CCGCCTGGC2821 A TTATGCCCA G TACATGACC T TATGGGACT T TCCTACTTG G CAGTACATC T ACGTATTAG2881 T CATCGCTAT T ACCATGGTC G AGGTGAGCC C CACGTTCTG C TTCACTCTC C CCATCTCCC2941 C CCCCTCCCC A CCCCCAATT T TGTATTTAT T TATTTTTTA A TTATTTTGT G CAGCGATGG3001 G GGCGGGGGG G GGGGGGGGG C GCGCGCCGG G CGGGGCGGG G CGGGGCGAG G GGCGGGGCG 3061 G GGCGAGGCG G AGAGGTGCG G CGGCAGCCA A TCAGAGCGG C GCGCTCCGA A AGTTTCCTT3121 T TATGGCGAG G CGGCGGCGG C GGCGGCCCT A TAAAAAGCG A AGCGCGCGG C GGGCGGGAG 3181 T CGCTGCGCG C TGCCTTCGC C CCGTGCCCC G CTCCGCCGC C GCCTCGCGC C GCCCGCCCC3241 G GCTCTGACT G ACCGCGTTA C TCCCACAGG T GAGCGGGCG G GACGGCCCT T CTCCTCCGG3301 G CTGTAATTA G CGCTTGGTT T AATGACGGC T TGTTTCTTT T CTGTGGCTG C GTGAAAGCC3361 T TGAGGGGCT C CGGGAGGGC C CTTTGTGCG G GGGGAGCGG C TCGGGGCTG T CCGCGGGGG3421 G ACGGCTGCC T TCGGGGGGG A CGGGGCAGG G CGGGGTTCG G CTTCTGGCG T GTGACCGGC3481 G GCTCTAGAG C CTCTGCTAA C CATGTTCAT G CCTTCTTCT T TTTCCTACA G CTCCTGGGC3541 A ACGTGCTGG T TATTGTGCT G TCTCATCAT T TTGGCAAAG A ATTGGATCC G CCACCATGG3601 T GAGCAAGGG C GAGGAGCTG T TCACCGGGG T GGTGCCCAT C CTGGTCGAG C TGGACGGCG3661 A CGTAAACGG C CACAAGTTC A GCGTGTCCG G CGAGGGCGA G GGCGATGCC A CCTACGGCA3721 A GCTGACCCT G AAGTTCATT T GCACCACCG G CAAGCTGCC C GTGCCCTGG C CCACCCTCG3781 T GACCACCCT G ACCTACGGC G TGCAGTGCT T CAGCCGCTA C CCCGACCAC A TGAAGCAGC3841 A CGACTTCTT C AAGTCCGCC A TGCCCGAAG G CTACGTCCA G GAGCGCACC A TCTTCTTCA3901 A GGACGACGG C AACTACAAG A CCCGCGCCG A GGTGAAGTT C GAGGGCGAC A CCCTGGTGA3961 A CCGCATCGA G CTGAAGGGC A TCGACTTCA A GGAGGACGG C AACATCCTG G GGCACAAGC4021 T GGAGTACAA C TACAACAGC C ACAACGTCT A TATCATGGC C GACAAGCAG A AGAACGGCA4081 T CAAGGTGAA C TTCAAGATC C GCCACAACA T CGAGGACGG C AGCGTGCAG C TCGCCGACC4141 A CTACCAGCA G AACACCCCC A TCGGCGACG G CCCCGTGCT G CTGCCCGAC A ACCACTACC4201 T GAGCACCCA G TCCGCCCTG A GCAAAGACC C CAACGAGAA G CGCGATCAC A TGGTCCTGC4261 T GGAGTTCGT G ACCGCCGCC G GGATCACTC T CGGCATGGA C GAGCTGTAC A AGTAAGAAT4321 T CGATATCAA G CTTATCGAT A ATCAACCTC T GGATTACAA A ATTTGTGAA A GATTGACTG4381 G TATTCTTAA C TATGTTGCT C CTTTTACGC T ATGTGGATA C GCTGCTTTA A TGCCTTTGT4441 A TCATGCTAT T GCTTCCCGT A TGGCTTTCA T TTTCTCCTC C TTGTATAAA T CCTGGTTGC4501 T GTCTCTTTA T GAGGAGTTG T GGCCCGTTG T CAGGCAACG T GGCGTGGTG T GCACTGTGT4561 T TGCTGACGC A ACCCCCACT G GTTGGGGCA T TGCCACCAC C TGTCAGCTC C TTTCCGGGA4621 C TTTCGCTTT C CCCCTCCCT A TTGCCACGG C GGAACTCAT C GCCGCCTGC C TTGCCCGCT4681 G CTGGACAGG G GCTCGGCTG T TGGGCACTG A CAATTCCGT G GTGTTGTCG G GGAAATCAT4741 C GTCCTTTCC T TGGCTGCTC G CCTGTGTTG C CACCTGGAT T CTGCGCGGG A CGTCCTTCT4801 G CTACGTCCC T TCGGCCCTC A ATCCAGCGG A CCTTCCTTC C CGCGGCCTG C TGCCGGCTC4861 T GCGGCCTCT T CCGCGTCTT C GCCTTCGCC C TCAGACGAG T CGGATCTCC C TTTGGGCCG4921 C CTCCCCGCA T CGATACCGT C GACCCGGGC G GCCGCTTCG A GCAGACATG A TAAGATACA4981 T TGATGAGTT T GGACAAACC A CAACTAGAA T GCAGTGAAA A AAATGCTTT A TTTGTGAAA5041 T TTGTGATGC T ATTGCTTTA T TTGTAACCA T TATAAGCTG C AATAAACAA G TTAACAACA5101 A CAATTGCAT T CATTTTATG T TTCAGGTTC A GGGGGAGAT G TGGGAGGTT T TTTAAAGCA5161 A GTAAAACCT C TACAAATGT G GTAAAATCG A TAAGGATCT T CCTAGAGCA T GGCTACGTA 5221 G ATAAGTAGC A TGGCGGGTT A ATCATTAAC T ACAAGGAAC C CCTAGTGAT G GAGTTGGCC 5281 A CTCCCTCTC T GCGCGCTCG C TCGCTCACT G AGGCCGGGC G ACCAAAGGT C GCCCGACGC 5341 C CGGGCTTTG C CCGGGCGGC C TCAGTGAGC G AGCGAGCGC G CAGCTGcat t aatgaatcg5401 g ccaacgcgc g gggagaggc g gtttgcgta t tgggcgct//pAAV-‐CAG-‐GFP其他腺病毒表达载体：pNTAP-‐Shuttle-‐B pAAV-‐CAG-‐RFPpAAV-‐CaMKIIa-‐EGFP pCTAP-‐Shuttle-‐ApAAV-‐CMV-‐Rluc pNTAP-‐Shuttle-‐ApAAV-‐CAG-‐GFP pBHGloxdelE13cre pCTAP-‐Shuttle-‐C pDC511pDC515 pDC312pAdTrack-‐CMV pAAV-‐IRES-‐hrGFP pShuttle-‐IRES-‐hrGFP-‐1 pShuttle-‐CMV-‐EGFP-‐C pacAd5 9.2-‐100 pacAd5 C MV-‐GFPpAAV-‐Syn-‐Rluc pCMV-‐MCSpShuttle-‐IRES-‐hrGFP-‐2 pShuttle-‐CMVpAAV-‐Syn-‐Rluc pAAV-‐hSyn-‐RFPpAAV-‐CMV-‐iRFP pAAV-‐UbCpDC415 pAAV-‐CAG-‐FlucpAAV-‐LacZ pNTAP-‐Shuttle-‐BpDC316 pDC516pAAV-‐CA pDC416pNTAP-‐Shuttle-‐C pDC311pDC512 pAAV-‐hrGFPpDC411 pAAV-‐RCpAdTrack pacAd5 C MVK-‐NpA pAdEasy-‐1 pDC315pAAV-‐MCS pShuttlepHelper pBApo-‐CMV-‐neo pShuttle-‐CMV-‐lacZ pBApo-‐CMVpAAV-‐TRE-‐Syn-‐Fluc pBApo-‐CMV-‐PurpAAV-‐minCMV-‐mCherry。