pAAV-‐CMV-‐iRFP编号 载体名称 北京华越洋VECT10016 pAAV-‐CMV-‐iRFPpAAV-‐CMV-‐iRFP载体基本信息载体名称: pAAV-‐CMV-‐iRFP质粒类型: 腺病毒载体；荧光蛋白报告载体高拷贝/低拷贝: 高拷贝克隆方法: 限制性内切酶，多克隆位点启动子: CMV载体大小: 5603 b p5' 测序引物及序列: pCAX-‐F (CAGCTCCTGGGCAACGTGC)3' 测序引物及序列: C-‐CMV-‐24 (TATTAGGACAAGGCTGGTGGGCAC) 载体标签: -‐-‐载体抗性: 氨苄青霉素筛选标记: 红外荧光蛋白iRFP克隆菌株: Stbl3 或 NEB S tble宿主细胞（系）: 常规哺乳动物细胞系备注: -‐-‐稳定性: 稳表达组成型/诱导型: 组成型病毒/非病毒: 腺病毒pAAV-‐CMV-‐iRFP载体质粒图谱和多克隆位点信息pAAV-‐CMV-‐iRFP载体序列:ORIGIN1 C CTGCAGGCA G CTGCGCGCT C GCTCGCTCA C TGAGGCCGC C CGGGCGTCG G GCGACCTTT 61 G GTCGCCCGG C CTCAGTGAG C GAGCGAGCG C GCAGAGAGG G AGTGGCCAA C TCCATCACT 121 A GGGGTTCCT G CGGCCGCAC G CGTGGAGCT A GTTATTAAT A GTAATCAAT T ACGGGGTCA 181 T TAGTTCATA G CCCATATAT G GAGTTCCGC G TTACATAAC T TACGGTAAA T GGCCCGCCT 241 G GCTGACCGC C CAACGACCC C CGCCCATTG A CGTCAATAA T GACGTATGT T CCCATAGTA 301 A CGTCAATAG G GACTTTCCA T TGACGTCAA T GGGTGGAGT A TTTACGGTA A ACTGCCCAC 361 T TGGCAGTAC A TCAAGTGTA T CATATGCCA A GTACGCCCC C TATTGACGT C AATGACGGT 421 A AATGGCCCG C CTGGCATTA T GCCCAGTAC A TGACCTTAT G GGACTTTCC T ACTTGGCAG 481 T ACATCTACG T ATTAGTCAT C GCTATTACC A TGGTGATGC G GTTTTGGCA G TACATCAAT 541 G GGCGTGGAT A GCGGTTTGA C TCACGGGGA T TTCCAAGTC T CCACCCCAT T GACGTCAAT 601 G GGAGTTTGT T TTGCACCAA A ATCAACGGG A CTTTCCAAA A TGTCGTAAC A ACTCCGCCC 661 C ATTGACGCA A ATGGGCGGT A GGCGTGTAC G GTGGGAGGT C TATATAAGC A GAGCTCGTT 721 T AGTGAACCG T CAGATCGCC T GGAGACGCC A TCCACGCTG T TTTGACCTC C ATAGAAGAC 781 A CCGGGACCG A TCCAGCCTC C GCGGATTCG A ATCCCGGCC G GGAACGGTG C ATTGGAACG 841 C GGATTCCCC G TGCCAAGAG T GACGTAAGT A CCGCCTATA G AGTCTATAG G CCCACAAAA 901 A ATGCTTTCT T CTTTTAATA T ACTTTTTTG T TTATCTTAT T TCTAATACT T TCCCTAATC961 T CTTTCTTTC A GGGCAATAA T GATACAATG T ATCATGCCT C TTTGCACCA T TCTAAAGAA 1021 T AACAGTGAT A ATTTCTGGG T TAAGGCAAT A GCAATATTT C TGCATATAA A TATTTCTGC 1081 A TATAAATTG T AACTGATGT A AGAGGTTTC A TATTGCTAA T AGCAGCTAC A ATCCAGCTA 1141 C CATTCTGCT T TTATTTTAT G GTTGGGATA A GGCTGGATT A TTCTGAGTC C AAGCTAGGC 1201 C CTTTTGCTA A TCATGTTCA T ACCTCTTAT C TTCCTCCCA C AGCTCCTGG G CAACGTGCT1261 G GTCTGTGTG C TGGCCCATC A CTTTGGCAA A GAATTGGGA T TCGAACATC G ATTGAATTC 1321 C CCGGGGATC T GCCGCCACC A TGGCGGAAG G ATCCGTCGC C AGGCAGCCT G ACCTCTTGA 1381 C CTGCGACGA T GAGCCGATC C ATATCCCCG G TGCCATCCA A CCGCATGGA C TGCTGCTCG 1441 C CCTCGCCGC C GACATGACG A TCGTTGCCG G CAGCGACAA C CTTCCCGAA C TCACCGGAC 1501 T GGCGATCGG C GCCCTGATC G GCCGCTCTG C GGCCGATGT C TTCGACTCG G AGACGCACA 1561 A CCGTCTGAC G ATCGCCTTG G CCGAGCCCG G GGCGGCCGT C GGAGCACCG A TCACTGTCG 1621 G CTtCACGAT G CGAAAGgAC G CAGGCTTCA T CGGCTCCTG G CATCGCCAT G ATCAGCTCA 1681 T CTtccTCGA G CTCGAGCCT C CCCAGCGGG A CGTCgccga g ccgcaggcg t tcttccgcc1741 g caCCAACAG C GCCATCCGC C GCCTGCAGG C CGCCGAAAC C TTGGAAAGC G CCTGCGCCG 1801 C CGCGGCGCA A GAGGTGCGG A AGATTACCG G CTTCGATCG G GTGATGATC T ATCGCTTCG 1861 C CTCCGACTT C AGCGGCGAA G TGATCGCAG A GGATCGGTG C GCCGAGGTC G AGTCAAAAC 1921 T AGGCCTGCA C TATCCTGCC T CAACCGTGC C GGCGCAGGC C CGTCGGCTC T ATACCATCA 1981 A CCCGGTACG G ATCATTCCC G ATATCAATT A TCGGCCGGT G CCGGTCACC C CAGACCTCA 2041 A TCCGGTCAC C GGGCGGCCG A TTGATCTTA G CTTCGCCAT C CTGCGCAGC G TCTCGCCCG 2101 T CCATCTGGA A TTCATGCGC A ACATAGGCA T GCACGGCAC G ATGTCGATC T CGATTTTGC 2161 G CGGCGAGCG A CTGTGGGGA T TGATCGTTT G CCATCACCG A ACGCCGTAC T ACGTCGATC 2221 T CGATGGCCG C CAAGCCTGC G AGCTAGTCG C CCAGGTTCT G GCCTGGCAG A TCGGCGTGA 2281 T GGAAGAGTG A GTCGACGCG G CCGCTCGAG C CTAAGCTTG C CTCGAGCAG C GCTGCTCGA 2341 G AGATCTACG G GTGGCATCC C TGTGACCCC T CCCCAGTGC C TCTCCTGGC C CTGGAAGTT2401 G CCACTCCAG T GCCCACCAG C CTTGTCCTA A TAAAATTAA G TTGCATCAT T TTGTCTGAC2461 T AGGTGTCCT T CTATAATAT T ATGGGGTGG A GGGGGGTGG T ATGGAGCAA G GGGCAAGTT 2521 G GGAAGACAA C CTGTAGGGC C TGCGGGGTC T ATTGGGAAC C AAGCTGGAG T GCAGTGGCA 2581 C AATCTTGGC T CACTGCAAT C TCCGCCTCC T GGGTTCAAG C GATTCTCCT G CCTCAGCCT2641 C CCGAGTTGT T GGGATTCCA G GCATGCATG A CCAGGCTCA G CTAATTTTT G TTTTTTTGG 2701 T AGAGACGGG G TTTCACCAT A TTGGCCAGG C TGGTCTCCA A CTCCTAATC T CAGGTGATC 2761 T ACCCACCTT G GCCTCCCAA A TTGCTGGGA T TACAGGCGT G AACCACTGC T CCCTTCCCT2821 G TCCTTCTGA T TTTGTAGGT A ACCACGTGC G GACCGAGCG G CCGCAGGAA C CCCTAGTGA 2881 T GGAGTTGGC C ACTCCCTCT C TGCGCGCTC G CTCGCTCAC T GAGGCCGGG C GACCAAAGG 2941 T CGCCCGACG C CCGGGCTTT G CCCGGGCGG C CTCAGTGAG C GAGCGAGCG C GCAGCTGCC 3001 T GCAGGGGCG C CTGATGCGG T ATTTTCTCC T TACGCATCT G TGCGGTATT T CACACCGCA 3061 T ACGTCAAAG C AACCATAGT A CGCGCCCTG T AGCGGCGCA T TAAGCGCGG C GGGTGTGGT 3121 G GTTACGCGC A GCGTGACCG C TACACTTGC C AGCGCCCTA G CGCCCGCTC C TTTCGCTTT 3181 C TTCCCTTCC T TTCTCGCCA C GTTCGCCGG C TTTCCCCGT C AAGCTCTAA A TCGGGGGCT3241 C CCTTTAGGG T TCCGATTTA G TGCTTTACG G CACCTCGAC C CCAAAAAAC T TGATTTGGG 3301 T GATGGTTCA C GTAGTGGGC C ATCGCCCTG A TAGACGGTT T TTCGCCCTT T GACGTTGGA 3361 G TCCACGTTC T TTAATAGTG G ACTCTTGTT C CAAACTGGA A CAACACTCA A CCCTATCTC3421 G GGCTATTCT T TTGATTTAT A AGGGATTTT G CCGATTTCG G CCTATTGGT T AAAAAATGA 3481 G CTGATTTAA C AAAAATTTA A CGCGAATTT T AACAAAATA T TAACGTTTA C AATTTTATG 3541 G TGCACTCTC A GTACAATCT G CTCTGATGC C GCATAGTTA A GCCAGCCCC G ACACCCGCC 3601 A ACACCCGCT G ACGCGCCCT G ACGGGCTTG T CTGCTCCCG G CATCCGCTT A CAGACAAGC 3661 T GTGACCGTC T CCGGGAGCT G CATGTGTCA G AGGTTTTCA C CGTCATCAC C GAAACGCGC 3721 G AGACGAAAG G GCCTCGTGA T ACGCCTATT T TTATAGGTT A ATGTCATGA T AATAATGGT 3781 T TCTTAGACG T CAGGTGGCA C TTTTCGGGG A AATGTGCGC G GAACCCCTA T TTGTTTATT 3841 T TTCTAAATA C ATTCAAATA T GTATCCGCT C ATGAGACAA T AACCCTGAT A AATGCTTCA3901 A TAATATTGA A AAAGGAAGA G TATGAGTAT T CAACATTTC C GTGTCGCCC T TATTCCCTT 3961 T TTTGCGGCA T TTTGCCTTC C TGTTTTTGC T CACCCAGAA A CGCTGGTGA A AGTAAAAGA 4021 T GCTGAAGAT C AGTTGGGTG C ACGAGTGGG T TACATCGAA C TGGATCTCA A CAGCGGTAA 4081 G ATCCTTGAG A GTTTTCGCC C CGAAGAACG T TTTCCAATG A TGAGCACTT T TAAAGTTCT 4141 G CTATGTGGC G CGGTATTAT C CCGTATTGA C GCCGGGCAA G AGCAACTCG G TCGCCGCAT 4201 A CACTATTCT C AGAATGACT T GGTTGAGTA C TCACCAGTC A CAGAAAAGC A TCTTACGGA 4261 T GGCATGACA G TAAGAGAAT T ATGCAGTGC T GCCATAACC A TGAGTGATA A CACTGCGGC 4321 C AACTTACTT C TGACAACGA T CGGAGGACC G AAGGAGCTA A CCGCTTTTT T GCACAACAT 4381 G GGGGATCAT G TAACTCGCC T TGATCGTTG G GAACCGGAG C TGAATGAAG C CATACCAAA 4441 C GACGAGCGT G ACACCACGA T GCCTGTAGC A ATGGCAACA A CGTTGCGCA A ACTATTAAC 4501 T GGCGAACTA C TTACTCTAG C TTCCCGGCA A CAATTAATA G ACTGGATGG A GGCGGATAA 4561 A GTTGCAGGA C CACTTCTGC G CTCGGCCCT T CCGGCTGGC T GGTTTATTG C TGATAAATC 4621 T GGAGCCGGT G AGCGTGGGT C TCGCGGTAT C ATTGCAGCA C TGGGGCCAG A TGGTAAGCC 4681 C TCCCGTATC G TAGTTATCT A CACGACGGG G AGTCAGGCA A CTATGGATG A ACGAAATAG 4741 A CAGATCGCT G AGATAGGTG C CTCACTGAT T AAGCATTGG T AACTGTCAG A CCAAGTTTA 4801 C TCATATATA C TTTAGATTG A TTTAAAACT T CATTTTTAA T TTAAAAGGA T CTAGGTGAA4861 G ATCCTTTTT G ATAATCTCA T GACCAAAAT C CCTTAACGT G AGTTTTCGT T CCACTGAGC4921 G TCAGACCCC G TAGAAAAGA T CAAAGGATC T TCTTGAGAT C CTTTTTTTC T GCGCGTAAT 4981 C TGCTGCTTG C AAACAAAAA A ACCACCGCT A CCAGCGGTG G TTTGTTTGC C GGATCAAGA5041 G CTACCAACT C TTTTTCCGA A GGTAACTGG C TTCAGCAGA G CGCAGATAC C AAATACTGT 5101 C CTTCTAGTG T AGCCGTAGT T AGGCCACCA C TTCAAGAAC T CTGTAGCAC C GCCTACATA5161 C CTCGCTCTG C TAATCCTGT T ACCAGTGGC T GCTGCCAGT G GCGATAAGT C GTGTCTTAC5221 C GGGTTGGAC T CAAGACGAT A GTTACCGGA T AAGGCGCAG C GGTCGGGCT G AACGGGGGG 5281 T TCGTGCACA C AGCCCAGCT T GGAGCGAAC G ACCTACACC G AACTGAGAT A CCTACAGCG 5341 T GAGCTATGA G AAAGCGCCA C GCTTCCCGA A GGGAGAAAG G CGGACAGGT A TCCGGTAAG 5401 C GGCAGGGTC G GAACAGGAG A GCGCACGAG G GAGCTTCCA G GGGGAAACG C CTGGTATCT 5461 T TATAGTCCT G TCGGGTTTC G CCACCTCTG A CTTGAGCGT C GATTTTTGT G ATGCTCGTC5521 A GGGGGGCGG A GCCTATGGA A AAACGCCAG C AACGCGGCC T TTTTACGGT T CCTGGCCTT 5581 T TGCTGGCCT T TTGCTCACA T GT//pAAV-‐CMV-‐iRFP其他腺病毒表达载体：pNTAP-‐Shuttle-‐B pAAV-‐CAG-‐RFPpAAV-‐CaMKIIa-‐EGFP pCTAP-‐Shuttle-‐ApAAV-‐CMV-‐Rluc pNTAP-‐Shuttle-‐ApAAV-‐CAG-‐GFP pBHGloxdelE13cre pCTAP-‐Shuttle-‐C pDC511pDC515 pDC312pAdTrack-‐CMV pAAV-‐IRES-‐hrGFP pShuttle-‐IRES-‐hrGFP-‐1 pShuttle-‐CMV-‐EGFP-‐C pacAd5 9.2-‐100 pacAd5 C MV-‐GFPpAAV-‐Syn-‐Rluc pCMV-‐MCSpShuttle-‐IRES-‐hrGFP-‐2 pShuttle-‐CMVpAAV-‐Syn-‐Rluc pAAV-‐hSyn-‐RFPpAAV-‐CMV-‐iRFP pAAV-‐UbCpDC415 pAAV-‐CAG-‐FlucpAAV-‐LacZ pNTAP-‐Shuttle-‐BpDC316 pDC516pAAV-‐CA pDC416pNTAP-‐Shuttle-‐C pDC311pDC512 pAAV-‐hrGFPpDC411 pAAV-‐RCpAdTrack pacAd5 C MVK-‐NpA pAdEasy-‐1 pDC315pAAV-‐MCS pShuttlepHelper pBApo-‐CMV-‐neo pShuttle-‐CMV-‐lacZ pBApo-‐CMVpAAV-‐TRE-‐Syn-‐Fluc pBApo-‐CMV-‐PurpAAV-‐minCMV-‐mCherry。