GGCATGCC CATGCATGCATG ACATGCATGCATGT
AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT
CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG
CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG
CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA
CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC
GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG
寡核苷酸序列
TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA
TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT
TTAATTAA GTTAATTAAC CCTTAATTAAGG
GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG
GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT
常用酶切位点表含保护 碱基
TTA standardization office【TTA 5AB- TTAK 08- TTA 2C】
酶 Acc I Afl III Asc I Ava I BamH I Bgl II BssH II BstE II BstX I Cla I
EcoR I Hae III Hind III Kpn I
10
50
10
75
Sca I Sma I Spe I Sph I Stu I Xba I Xho I Xma I
GAGTACTC AAAAGTACTTTT
CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG
0
0
0
0
50
>90
GGGT(A/T)ACCC
0
10
AACTGCAGAACCAATGCATTGG
0
0
AAAACTGCAGCCAATGCATTGGAA
25
50
CTGCAGAACCAATGCATTGGATGCAT
25
>90
CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG
0
0
0
0
>90
>90
50
50
GGAATTCC CGGAATTCCG CCGGAATTCCGG
>90
>90
>90
>90
>90
>90
GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA
>90
>90
>90
>90
>90
>90
CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG
0
0
0
0
10
>90
CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
50
>90
>90
>90
>90
>90
CGGATCCG CGGGATCCCG CGCGGATCCGCG
10
25
>90
>90
>90
>90
CAGATCTG GAAGATCTTC GGAAGATCTTCC
0
0
75
>90
25
>90
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
寡核苷酸序列
切割率%
2 hr
20 hr
GGTCGACC CGGTCGACCG CCGGTCGACCGG
0
0
0
0
0
0
CACATGTG CCACATGTGG CCCACATGTGGG
0
0
>90
>90
>90
>90
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
>90
>90
>90
>90
>90
75
GGGTACCC GGGGTACCCC CGGGGTACCCCG
0
0
>90
>90
>90
>90
Mlu I Nco I Nde I
Nhe I 酶 Not I
Nsi I Pac I Pme I
Pst I
Pvu I Sac I Sac II Sal I
GACGCGTC CGACGCGTCG
CCCATGGG CATGCCATGGCATG
CCGATCGG ATCGATCGAT TCGCGATCGCGA
CGAGCTCG
GCCGCGGC TCCCCGCGGGGA
GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCG
G ACGCGTCGACGTCGGCCATAGCGGCCGCG
GAA
0
0
25
50
0
0
50
75
0
0
0
0
0
0
0
0
75
>90

10
25
10
50
切割率%
2 hr
20 hr
0
0
10
10
10
10
25
90
25
>90
10
>90
>90
>90
0
0
0
25
0
>90
0
0
0
25
0
50
75
>90
0
0
10
10
>90
>90
>90
>90
0
0
0
0
10
25
0
10
10
10
0
0
50
>90
0
0
10
25
75
75
0
10
0
10
10
50
>90
>90
10
>90
10
>90
0
50
0
50
0
0
0
25
10
50
>90
>90
>90
>90
>90
>90
0
0
>90
>90
75
>90
75
>90
0
0
10
25
10
75
0
0
25
75
50
>90
>90
>90