8 10 12 18 20 22 8 10 12 12 16 20 24 28 12 22 8 10 12 8 10 12 24 8 14 22 24 26 8 10 12 8 8 12 28 30 32 8 12 6 8 10 12 8 10 12 14
0 0 0 0 75 75 0 10 10 0 10 10 25 25 10 >90 0 0 0 0 0 0 75 0 10 >90 >90 0 0 10 0 10 0 50 0 10 10 10 75 0 0 10 >90 10 10 0 0
% Cleavage 2 hr 20 hr 0 0 0 0 0 0 0 0 >90 >90 >90 >90 >90 >90 >90 >90 >90 >90 50 >90 >90 >90 >90 >90 10 25 >90 >90 >90 >90 0 0 75 >90 25 >90 0 0 0 0 50 >90 0 10 0 0 25 50 25 >90 0 0 0 0 >90 >90 50 50 >90 >90 >90 >90 >90 >90 >90 >90 >90 >90 >90 >90 0 0 0 0 10 75 0 0 >90 >90 >90 >90 0 0 25 50 0 0 50 75
XhoI
XmaI
GGCATGCC CATGCATGCATG ACATGCATGCATGT AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA
NdeI
NheI
NotI
NsiI PacI
PmeI
PstI
PvuI
SacI SacII SalI
ScaI SmaI
SpeI
CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT TTAATTAA GTTAATTAAC CCTTAATTAAGG GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT CCGATCGG ATCGATCGAT TCGCGATCGCGA CGAGCTCG GCCGCGGC TCCCCGCGGGGA GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA GAGTACTC AAAAGTACTTTT CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG
0 0 0 0 >90 >90 0 25 50 0 10 10 90 >90 >90 >90 0 25 >90 0 25 50 >90 0 10 >90 >90 0 0 25 10 10 0 >90 0 50 75 25 75 10 10 50 >90 >90 >90 50 50
SphI
StuI
XbaI
8 12 14 8 10 12 8 10 12 14 8 10 12 8 10 12 14
0 0 10 >90 >90 >90 0 >90 75 75 0 10 10 0 25 50 >90
0 25 50 >90 >90 >90 0 >90 >90 >90 0 25 75 0 75 &
AscI
AvaI
BamHI
BglII
BssHII
BstEII BstXI
ClaI
EcoRI
HaeIII
HindIII
KpnI
MluI NcoI
Chain Length 8 10 12 8 10 12 8 10 12 8 10 12 8 10 12 8 10 12 8 10 12 9 22 24 27 8 8 10 12 8 10 12 8 10 12 8 10 12 8 10 12 8 10 8 14
Enzyme AccI
Oligo Sequence GGTCGACC CGGTCGACCG CCGGTCGACCGG CACATGTG CCACATGTGG CCCACATGTGGG GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA CGGATCCG CGGGATCCCG CGCGGATCCGCG CAGATCTG GAAGATCTTC GGAAGATCTTCC GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA GGGT(A/T)ACCC AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG GGAATTCC CGGAATTCCG CCGGAATTCCGG GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG GGGTACCC GGGGTACCCC CGGGGTACCCCG GACGCGTC CGACGCGTCG CCCATGGG CATGCCATGGCATG