but requires temperature of 170ºC [ 340 ºF]for about 2 hr.
•灭菌所需时间到后，切断电源，让灭菌锅内温 度自然下降，当压力表的压力降至0时，打开排 气阀，旋松螺栓，打开盖子，取出灭菌物品。如 果压力未降到0时，打开排气阀，就会因锅内压 力突然下降，使容器内的培养基由于内外压力不 平衡而冲出烧瓶口或试管口，造成棉塞沾染培养 基而发生污染。
婴幼儿体格生长
Lab-1培养基的配制与灭菌
2 Sterilization
– destruction of all forms of microbial life, including endospores.
Physical Methods
—Moist heat
Autoclaving • Action - protein denaturation. • Very effective method of sterilization; at
•将取出的灭菌培养基放入 37 ℃温箱培养24 h， 经检查若无杂菌生长，即可待用。
谢谢
about 15 psi（Ib/in2 ） of pressure (121ºC [ 250ºF]), all vegetative cells and their endospores are killed i heat
Direct flaming • example: Inoculation loop Hot-air sterilization • Baking in oven • Very effective method of sterilization,