Thermocycling is based on 96 well plate which remains open until last samples are loaded Requires proprietary master mix
Up to 10,000,000 droplets generated Single use disposable flow path
Total time ~4.5 hours excluding analysis
4
Company Confidential
RaRinaDinaDncaencTeecThenchonloogloiegsi™es
Selling Against QuantStudio 12K Flex OpenArray
RaRinaDinaDncaencTeecThenchonloogloiegsi™es
Selling Against BioRad Workflow
Competitor
RainDrop Solution
Take Home
20,000 droplets generated
Cross contamination of samples from reused sampling probe and flow path Vacuum delivery of samples can produce inconsistently sized droplets
Competitor
RainDrop Solution
Take Home
3072 maximum partitions
Up to 48 pipetting steps per sample Capillary filling of tips and array can fail to fill partitions
Fill with immersion fluid
Screw to seal
Place in holder
Merge sample info on instrument
Load into instrument and run ~4 hours
Analyze data using Digital Suite
Works with several master mixes
Leading edge science demands flexibility
3
Company Confidential
RaRinaDinaDncaencTeecThenchonloogloiegsi™es
Applied Biosystems QuantStudio™ 12K Flex by Life Technologies
Closed tube design eliminates the potential for contamination and spills. 8 sample strip design reduces waste and speeds time to first result
Contamination control is important in digital PCR
Superior Value Proposition for RainDrop™ Digital PCR
Partitions Multiplexing Dynamic range Sensitivity Closed-tube System Workflow Proprietary Reagents Throughput/Shift Instrument price Price per sample
Monitor droplet size during generation and adjust pressure as necessary to maintain consistent size
Consistency in processing is the basis for consistency in results
20,000
3,072
2-plex
2-plex
>3 lYes
Yes


Yes
Yes
18?
12
$90,000
$170,000
$15 - $35
$150
Company Confidential
RainDance Technologies™
QX100™ Droplet Digital™ PCR System
Load samples, MM, and assay into 384 well plate for AccuFill, seal with foil, then cut
Place 384 well plate, tips, and array in AccuFill
Place in plate sealer and apply lid – 90 seconds!
Works with several master mixes
3072 partitions don’t give you single molecule counting – reliance on Poisson statistics to generate results More pipetting, more time, more money, more error Consistency in processing is the basis for consistency in results
20K droplets don’t give you single molecule counting – reliance on Poisson statistics to generate results. Limited to duplexing.
You work hard to get the highest quality results…don’t risk this sample’s answer with the prior one
Requires proprietary master mix
Up to 10,000,000 droplets generated
-One pipetting step per sample -Closed loop pressure control allows RainDrop Source to dropletize the entire sample in each lane simultaneously Samples are in sealed PCR tubes, can not evaporate or spill, and can be stored up to 3 days if necessary Walk away workflow with minimal hands on time
Up to 10,000,000 droplets generated
One pipetting step per sample
Monitor droplet size during generation and adjust pressure as necessary to maintain consistent size Samples are in sealed PCR tubes, can not evaporate or spill, and can be stored up to 3 days if necessary
Once loaded arrays are fragile – sample can evaporate, get flushed out of partitions, or get jarred free, and break down if not run immediately Requires proprietary master mix
Competitor
RainDrop Solution
Take Home
Up to 20,000 partitions
Pipetting/squeegee loading of sample difficult, requires loader, uses capillary action
Pouring immersion fluid and snapping lid can flush samples out of partitions Must sit at instrument to feed chips in every couple minutes
QuantStudio™ 3D Digital PCR System
Mix sample, assay, master mix, and pipet onto chip
Place onto loader, use squeegee to distribute across chip
Pour immersion fluid on chip, snap lid into plate
Place in thermal cycler ~3.5 hours
Insert chip into instrument to read, ~2 min
Transfer data to computer
Set ROX, FAM, and VIC baselines
Future software for Rare Allele and Absolute Quantitation
Transfer samples into plate
Seal plate
Remove plate from cycler
2