细胞工程期末复习资料一.选择题( ) 1. RNA interference (RNAi) refers to a phenomenon that ＿＿＿inhibits expression of genes homologous to that ＿＿＿.A: single-stranded DNA, ssDNA B: double-stranded RNA, dsRNA C: single-stranded RNA, ssRNA D: double-stranded DNA, dsDNA ( ) 2. The Nobel Prize in Physiology or Medicine for 2007 was awarded jointly to Mario R. Capecchi, Martin J. Evans and Oliver Smithies, for their discoveries of ＿＿.A: principles for introducing specific gene modifications in mice by the use of embryonic stem cellsB: the bacterium Helicobacter pylori and its role in gastritis and peptic ulcer disease C: RNA interference - gene silencing by double-stranded RNAD: human papilloma viruses and human immunodeficiency virus( )3. The Nobel Prize in Physiology or Medicine for 2009 was awarded to scientists Elizabeth H. Blackburn, Carol W. Greider, and Jack W. Szostak for their work in the discovery of＿＿＿.A．principles for introducing specific gene modifications in mice by the use of embryonic stem cellsB．how chromosomes are protected by telomeres and the enzyme telomerase C．RNA interference - gene silencing by double-stranded RNAD．human papilloma viruses and human immunodeficiency virus( )4.贴壁型细胞附着在某一固相支持物表面才能生长，贴壁后呈现特定的形态特征。

如图所示的细胞类型称为＿＿＿.A:成纤维细胞型B:游走细胞型C:上皮型细胞D:多型细胞型（）5. 关于动物细胞培养要使用血清，下列说法正确的是＿＿＿。

A:血清使用前必须灭活B:到目前为止，所有动物细胞培养都需要使用血清C:血清中含有抗蛋白酶成分，起到中和作用，可以用血清来终止胰蛋白酶的消化。

D:血清的使用有利于实验和生产的标准化（）6. 目前，全球已有大约多少人通过体外受精技术出生？A 4万B 40万C 400万D 4000万（）7. Which of the following physical characteristics did Vincent NOT have to hide or alter in order to pass off as Jerome?A. His heightB. Eye-colorC. Left-handednessD. His accent (speech)（）8. After the mission director was murdered, what did the police find of Vincent’s near the crime scene that led them to suspect him of the crime?A. His eyelashB. His heart monitorC. His walletD. His fingerprint（）9. How did Jerome/Eugene become paralyzed?A. He was attacked by a drunk in a barB. He fell off a buildingC. He was hit by a carD. He was injured in a freak swimming accident （）10. How was the murderer identified in the end?A. The murderer was never identifiedB. From video surveillance footageC. From saliva found in the victim’s eyeD. A witness to the murder came forward（）11. 在电影《千钧一发》（Gattaca）中，没有使用以下哪种方法鉴别一个人的身份？A：血液B：尿液C：头发D：漱口水（）12. 在电影千钧一发（Gattaca）中，男主人公掉了什么东西而让警察怀疑自己是凶手？A：头发B：眼睫毛C：胡子D：腿毛（）13. 《千钧一发》（Gattaca）是一部讲述有关的电影?A 克隆人B 环境保护C 基因歧视D 干细胞（）14. What was the maximum age limit that the doctors gave to Vincent at his birth?A 30 yearsB 35 yearsC 40 yearsD 50 years（）15. What do the letters or terms, in the title 'GATTACA' represent?A. It is a term meaning that people are discriminated against.B. It is created solely from the characters names.C. It is created from the letters used to label nucleotide bases of DNA.（）16. At the end of the movie, Jerome/Eugene gave Vincent an envelope and told him not to open it until he was “upstairs” (in space). What did Eugene give to Vincent?A. His silver swimming medalB. MoneyC. A lock of his hairD. A letter thanking Vincent( )17.What is a stem cell?A: A cell that can make copies of itself AND make more specialized types of cell B: A cell that helps to fight against infectionsC: A cell that is specializedD: A cell that can produce all the cell types of the body( )18.Where can scientists obtain stem cells?A: Only from an embryoB: Only from adult tissuesC: Only from the brainD: From an embryo or adult tissues( )19.A blastocyst is…A: A very early stage embryoB: A type of stem cellC: Part of the blood systemD: A type of brain cell( )20.Embryonic stem cells can differentiate into which types of cell?A: Only brain stem cells and specialized brain cellsB: All types of specialized cells in the bodyC: Only cells that can produce insulinD: Only cells that can produce artificial skin( ) 21.Which of the following is not a property of adult stem cells?A: They are found among the differentiated cells in a tissue or an organ.B: They can only differentiated into that particular tissues or organs where they are originally from.C: They can proliferate without differentiating for a long period.D: They can differentiate into an intermediate cell type or types call called a progenitor cell before they achieve their fully differentiated state.( )22.Which of the following is NOT true about stem cell?A: Stem cell can specialize into different types of cells and tissues.B: Stem cell can retain a copy of itself.C: Stem cell is aneuploid, typically having many extra copies of various chromosomes.D: Stem cell can divide and differentiate under appropriate condition or environment, when there is presence of certain growth factors and other cell regulation signals. ( )23.Which of the following statements about reproductive and therapeutic cloning is false?A. Reproductive cloning could be used effectively for repopulating endangered species or to help make breeding of specific animals easier.B. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal.C. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in stem cell research.D. Dolly was created by therapeutic cloning.( )24.What method is used today to clone an animal?A. embryo splittingB. somatic cell nuclear transferC. blastomere separationD. Intracytoplasmic sperm injection（）25.《第六日》（The 6th Day ）是一部有关﹍的电影？A 克隆人B 环境保护C 基因歧视D 干细胞（）26._____ is a 2000 action film directed by Roger Spottiswoode and starring Arnold Schwarzenegger. The action hero plays family man Adam Gibson, who is cloned against his will in the future of 2015.A. GattacaB. AvatarC. The 6th DayD. The Island（）27.Which gene transfer technique involves a tiny needle which is used to inject DNA into a cell lacking that DNA sequence?A) spermatogonial stem cells transplantationB) liposome transferC) microinjectionD) gene targeting of embryo stem (ES) cells（）28..Genetic engineering manipulates gene products at the level of theA) protein. B) amino acid. C) DNA. D) RNA.（）29.Gene targeting is done on a(an)﹍？A) sperm cell. B) egg cell. C) fertilized ovum. D) embryonic stem cell. ( )30..A mule is a sterile hybrid from a ( ) and a ( ).A. female horse male donkeyB. male horse female donkeyC. female horse male monkeyD. female monkey male horse二．填空1. _____：___________________ _____________________①The other half of the 2008 Nobel Prize is shared jointly by a pair of French researchers, Françoise Barré-Sinoussi and Luc Montagnier, for their discovery of.②The cloning of enabled studies of its origin and evolution.③Successful anti-retroviral therapy results in life expectancies for persons with ____ infection now reaching levels similar to those of uninfected people.2. _____：___________________ _____________________①The 2008 Nobel Prize in Chemistry was awarded to scientists who advanced the use of ，an indispensable tool in molecular biology.②The man who discovered the gene, however, is doing something quite different these days.③With the aid of, researchers have developed ways to watch processes that were previously invisible, such as the development of nerve cells in the brain or how cancer cells spread.④are used in experiments to observe cell dynamics and behaviour----their fluorescent glow allows scientists to visualise processes inside cells._____：___________________ _____________________is a relatively new technology utilizing labeled DNA probes to detect or confirm gene or chromosome abnormalities that are generally beyond the resolution of routine Cytogenetics.3. _____：___________________ _____________________①is a solution commonly used in biological research.②has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances.4. _____：___________________ _____________________①When the media is needed, add 50-ml of and 5-ml of antibiotics to one of the portions. This completes the media for use.②I need to decrease the concentration of in my media. Normally, I use 10% _____ and I would like to use 1%. Does it cause any problem for cell growth?5. ATCC : ___________________ _____________________6._____：___________________ _____________________①The National Institutes of Health , a part of the U.S. Department of Health and Human Services, is the primary Federal agency for conducting and supporting medical research.②Helping to lead the way toward important medical discoveries that improve people's health and save lives, scientists investigate ways to prevent disease as well as the causes, treatments, and even cures for common and rare diseases.③Composed of 27 Institutes and Centers, the provides leadership and financial support to researchers in every state and throughout the world.7. _____：___________________ _____________________又称“第二代试管婴儿”①_____ can be effective when as few as one sperm can be obtained .②Studies published in scientific journals have shown that children resulting from ______are more likely than other children to have a genetic abnormality.8._____：___________________ _____________________①The size of a DNA piece that can be reproduced in ______ is 10 times greater than that which can be reproduced in bacteria.②Techniques for cloning genomic DNA into______ make it possible to analyze very long DNA sequences like human genes.9._____：___________________ _____________________①In support of the functional analysis of genes, the _____s are very useful for making transgenic animals with segments of human DNAs.②_____ is a vector used to clone DNA fragments (100- to 300-kb insert size; average, 150 kb) in Escherichia coli cells.三.名词解释1.(green fluorescent protein) :2. Contact Inhibition：3. Barr body：4. Primary Culture：5. sperm capacitation：6.ermatogenesis：8.In Vitro Fertilization：9.embryonic stem cells ：10.induced pluripotent stem cell：11.Hematopoietic stem cell：12.Hematopoietic stem cell transplantation：13.Mesenchymal Stem Cell ：14.Spermatogonial stem cells：15.Tissue Engineering：16.Gene targeting：17. mammary gland bioreactor：18.gynogenesis：19.Androgenesis：20.artificial chromosome：四.简答题1.简介2005-2011年诺贝尔生理医学奖及2008年诺贝尔化学奖奖。