？ ？
7 Development 139, 3-14, 2012
Two possible mechanisms for the generation of multiple cell types and polarity
8 Development 139, 3-14, 2012
Mouse trophectoderm distinguish from inner cell mass regulated by Hippo signaling pathway
9 Dev Cell 16, 398-410, 2009
Cell position-dependent differentiation mediated by Hippo signaling
10 Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
？
15 Development 139, 3-14, 2012
Emerging asymmetry and establishment of anteriorposterior polarity in the E5.75 mouse embryo
Axis of Proximal & Distal
Axis of Anterior-Posterior
23 Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
24 Development 139, 3-14, 2012
Early mouse embryo development and lineage segregation
16
Cell movements in early mouse embryos
Distal VE to AVE
Epiblast to posterior
Extraembryonic
Epiblast to
ectoderm to posterior posterior
epiblast
17
Movement of Otx2-positive cells from DVE to AVE
3.5dpc ICM
4.5dpc early epiblast
Nervous system
Skin and hair
Heart
……
Muscle
5.5dpc late epiblast
liver
……
Lungs
Primitive streak
6.5dpc
25
Rapid proliferation during gastrulation: dramatic signaling and gene expression changes
VE: visceral endoderm AVE: anterior VE DVE: distal VE
(late) Epiblast
Anterior neuroectoderm
5
Cell lineages in early mouse embryo
Morula
Primitive endoderm
？
11 Development 139, 3-14, 2012
Early mouse development
E4.5
E3.5 ICM
12
‘‘Salt and Pepper’’ expression of Nanog and Gata6 in the ICM of E3.5 blastocysts
19 Dev Cell 10, 451-459, 2006
Two types of Lefty1+ cell in mouse embryos between E3.5 and E4.5
20 Nat Cell Biol 13, 743-752, 2011
Distinct origins of DVE and AVE
21 Nat Cell Biol 13, 743-752, 2011
Origins of the dorsal and anterior visceral endoderm
22 Development 139, 3-14, 2012
Signaling pathways involved in A-P axis determination
小鼠早期胚胎发育
1
体轴形成和原肠运动 Axis formation & Gastrulation
2
小鼠早期胚胎发育
3
Early mouse development
Preimplantation
4
Early mouse development
Postimplantation
(early) Epiblast
13 Dev Cell 10, 615-624, 2006
Segregation and relocation of epiblast and PrE cells
14 Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
Inner Cell Mass
Trophectoderm
Epiblast
Parietal endoderm
Visceral endoderm
Mesendoderm
Ectoderm
Definitive endoderm Mesoderm Epidermis
CNS
6
Mouse embryo development from fertilization to gastrulation ？
5.5 dpc
5.75 dpc
6.0 dpc
6.25 dpc
Otx2 VEcis-lacZ
Otx2 KI-lacZ
DVE: distal visceral endoderm; AVE: anterior viscerammetric expression of Lefty1 transgenes in peri-implantation mouse embryos