(early) Epiblast
VE: visceral endoderm AVE: anterior VE DVE: distal VE
(late) Epiblast
Anterior neuroectoderm
Cell lineages in early mouse embryo
Morula
Primitive endoderm
Segregation and relocation of epiblast and PrE cells
Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
？
Development 139, 3-14, 2012
Dev Cell 16, 398-410, 2009
Cell position-dependent differentiation mediated by Hippo signaling
Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
Emerging asymmetry and establishment of anteriorposterior polarity in the E5.75 mouse embryo
Axis of Proximal & Distal
Axis of Anterior-Posterior
Cell movements in early mouse embryos
Inner Cell Mass
Trophectoderm
Epiblast
Parietal endoderm
Visceral endoderm
Mesendoderm
Ectoderm
Definitive endoderm Mesoderm Epidermis
CNS
Mouse embryo development from fertilization to gastrulation ？
？ ？
Development 139, 3-14, 2012
Two possible mechanisms for the generation of multiple cell types and polarity
Development 139, 3-14, 2012
Mouse trophectoderm distinguish from inner cell mass regulated by Hippo signaling pathway
5.5 dpc
5.75 dpc
6.0 dpc
6.25 dpc
Otx2 VEcis-lacZ
Otx2 KI-lacZ
DVE: distal visceral endoderm; AVE: anterior visceral endoderm
Asymmetric expression of Lefty1 transgenes in peri-implantation mouse embryos
Distal VE to AVE
Epiblast to postctoderm to posterior
epiblast
Epiblast to posterior
Movement of Otx2-positive cells from DVE to AVE
Dev Cell 10, 451-459, 2006
Two types of Lefty1+ cell in mouse embryos between E3.5 and E4.5
Nat Cell Biol 13, 743-752, 2011
Distinct origins of DVE and AVE
Fossat, N., et al. (2012) Cell Cycle 11: 26-32
？
Development 139, 3-14, 2012
Early mouse development
E4.5
E3.5 ICM
‘‘Salt and Pepper’’ expression of Nanog and Gata6 in the ICM of E3.5 blastocysts
Dev Cell 10, 615-624, 2006
小鼠早期胚胎发育
景乃禾
中国科学院上海生物化学与细胞生物学研究所
体轴形成和原肠运动 Axis formation & Gastrulation
小鼠早期胚胎发育
Early mouse development
Preimplantation
Early mouse development
Postimplantation
Nat Cell Biol 13, 743-752, 2011
Origins of the dorsal and anterior visceral endoderm
Development 139, 3-14, 2012
Signaling pathways involved in A-P axis determination
Development 139, 3-14, 2012
Mouse embryo development from fertilization to gastrulation
Development 139, 3-14, 2012
Early mouse embryo development and lineage segregation
3.5dpc ICM
4.5dpc early epiblast
Nervous system
Skin and hair
Heart
……
Muscle
5.5dpc late epiblast
liver
……
Lungs
Primitive streak
6.5dpc
Rapid proliferation during gastrulation: dramatic signaling and gene expression changes