B cell differentiation with anatom ical sites of various stages
Could you recognize cellular elements as follows in lymph node?
T-zone
Germinal center Mantle zone Marginal zone
Even more, the molecules in the related cells are the key, especially involved in the development and differentiation of lymphoid & hematopoietic cells
B cleaved cell
B non-cleaved cell
T lymphocyte
T lmmunocyte
Memory cell Plasma cell
Paradigm of Lymphocyte transform
by morphology and immunotyping considering molecular events
Thymus-depending zone
Capsular and afferent lymphatics
Reticulum supporting lymphatic tissue
Cortex
Subcapsular sinus
Cortbeculae Medullary cords
分子病理学技术进展及临床应用
内容:
一、临床分子病理学方法1 二、当前分子病理学技术在临床的实际应用 三、分子病理学临床应用存在的问题及对策思考
来自临床的问题
Contemporary Understanding of Carcinogenesis by molecular biology
as our introduction
Understanding of B cell differentiation until 2005 by H Stein
Bone Marrow
T cell-rich zone
Germinal Center
Precursor B Lymphoblast
Somatic Hypermutation/ Affinity Maturation
Centrocyte T cell Macrophage FDC
Proliferation zone
Selected for apoptosis
On-going mutation
Update to B-lymphocyte development model in 2000’s
B cell differentiation by immuno-markers
V(D)J recombination
Naive B cell
Intact BCR
Antigen
BCL2 ↓ BCL6 ↑
B B
B
B DC
BT
Increased Affinity
B
Decreased Affinity
Class Switching IGM → IgG, IgA
DC
T
B
B
BCL6 ↓ BCL2 ↑ IRF4 ↑ B
Hilus
Medullary sinuses Efferent lymphatic vessels
Could you recognize the following cellular elements in lymph node?
T-zone
Germinal center Mantle zone Marginal zone
Lennert’s Presumption based on 80’s of last Century
Mantle zone
Germinal center Dark zone Light zone
Mantle zone Marginal zone
Centroblast Blast Immuoblast Macrophage FDC
More than 100 years ago (1830), British physician Thomas Hodgkin discovered a type of Lymphoma with his naked eyes.
In the time, obviously Thomas Hodgkin did never know R-S cells in his cases before the microscope available in clinic.
Understanding based on 70’s of last Century
Only by morphology
Lymphocyte transform in center of Follicle Interfollicle zone
FDC B lymphocyte
B lmmunocyte
That is the beginning of our story today.
Understanding of Lymphocyte Development
1950s-2010
1. structure and function of lymphoid tissue
Germenal Center(GC)：structure，function，transformation
While at present time, for us, what can be down with our naked eyes rather then with microscope or modern molecular techniques?
The cellular elements in lymphoid & hematopoietic tumors are the key to understand and diagnose their diseases.
Apoptosis
No BCR