Invitrogen中国测序通用引物序列引物名称序列(5'-3')M13R CAG GAA ACA GCT A TG ACCM13F TGT AAA ACG ACG GCC AGTM13F(-47) CGC CAG GGT TTT CCC AGT CAC GAC M13R(-48) AGC GGA TAA CAA TTT CAC ACA GGA M13(-96) CCC TCA TAG TTA GCG TAA CGSP6 A TT TAG GTG ACA CTA TAGT7 TAA TAC GAC TCA CTA TAG GGT7 terminator TGC TAG TTA TTG CTC AGC GGT3 A TT AAC CCT CAC TAA AGG GA pGEX-4T-5' GGG CTG GCA AGC CAC GTT TGG TG pGEX-4T-3' CCG GGA GCT GCA TGT GTC AGA GG GLp1 TGT A TC TTA TGG TAC TGT AAC TG GLp2 CTT TA T GTT TTT GGC GTC TTC CA RVp3 CTA GCA AAA TAG GCT GTC CCRVp4 GAC GA T AGT CA T GCC CCG CG pcDNA3.1R TAG AAG GCA CAG TCG AGG PinPoint primer CGT GAC GCG GTG CAG GGC G pCMV-F TCT AAA AGC TGC GGA A TT GT pCMV-R TCCAAACTCA TCAA TGTA TCpTRC99C-F: TTG CGC CGA CA T CA T AACpTRC99C-R: CTGCGTTCTGA TTTAA TCTGpCEP-F: AGA GCT CGT TTA GTG AAC CGEBV-R : GTG GTT TGT CCA AAC TCA TC pIRES2-EGFP.P5’:GTA GGC GTG TAC GGT GGG AG pIRES2-EGFP.P3’: AAC GCA CAC CGG CCT TA T TC3'AD: AGA TGG TGC ACG A TG CAC AGCMV -F CGC AAA TGG GCG GTA GGC GTGS1 CAA CGT GAA AAA A TT A TT A TT CGC S6 GTA AA T GAA TTT TCT GTA GTA GG5`AOX1 GAC TGG TTC CAA TTG ACA AGC3`AOX1 GCA AA T GGC A TT CTG ACA TCCα-Factor TAC TA T TGC CAG CA T TGC TGCGAL4 AD TAC CAC TAC AA T GGA TGpACT2-R GTGCACGA TGCACAGTTGAApB42ADF: CCA GCC TCT TGC TGA GTG GAG A TGpB42ADR: AAG CCG ACA ACC TTG A TT GGA G pEGFP-N-5’: TGG GAG GTC TA T A TA AGC AGA G pEGFP-N-3’: CGT CGC CGT CCA GCT CGA CCA G pEGFP-C-5’: CA T GGT CCT GCT GGA GTT CGT G pEGFP-C-3' : TA T GGC TGA TTA TGA TCA GTPBV220F: AAG AAG GGC AGC A TT CAA AGPBV220R: CTG CGT TCT GA T TTA A TC TGU6 A TG GAC TA T CA T A TG CTT ACC GTA2.0rev primer: AGG CGA TTA AGT TGG GTA3.0rev: GAG TTA GCT CAC TCA TTA GGCS.tag GAA CGC CAG CAC A TG GAC5'TriplEx2 CTC CGA GA T CTG GAC GAG CRVP4 GAC GA T AGT CA T GCC CCG CGRVP3 CTA GCA AAA TAG GCT GTC CCpQE30-R GTT CTG AGG TCA TTA CTG GpQE30-F TGA GCG GA T AAC AA T TTC ACpEF-F TCA AGC CTC AGA CAG TGG TTC pDSRED-N1-R TGA AGC GCA TGA ACT CCT TG pDsRED-express-C1-F TCC CAC AAC GAG GAC TAC ACpCMV5R A TT A TA GAG GAC ACC TAG TCpCMV5F TTC CAA AA T GTC GTA A TA ACP5' TGC GTA CTG CGG TGA TCA ACP3' CTG CAA GGC GA T TAA GTT GGBAC2 ACG CAC AGA A TC TAG CGC TTBAC1 AAC CA T CTC GCA AA T AAA TA3'BD: TAA GAG TCA CTT TAA AA T TTG TA T AC pTarget.F CCA GGA TTT TCC CAG TCA C pTarget.R GGC TTT ACA CTT TA T GCT TCT7(17base) ACA TCC ACT TTG CCT TTC TCpY es2.R TCG GTT AGA GCG GA T GTGGAL4 BD TCA TCG GAA GAG AGT AGpAS2-1.R AAG AGT TAC TCA AGA ACA AGA A pFlag-CMV-F: GGT AGG CGT GTA CGG TGGpFlag-CMV-R: GCA CTG GAG TGG CAA CTTpIRES-F: CTT ACT GAC A TC CAC TTT GC pIRES.R: CAC TGC A TT CTA GTT GTG GTP1 CCA GGC TTT ACA CTT TA T GCP2 GCG A TT AAG TTG GGT AAC GCpLEXA-F CGT CAG CAG AGC TTC ACC A TpLEXA-R TAA AAC CTA AGA GTC ACT TTpLNCX-F AGC TCG TTT AGT GAA CCG TCA GA T CG pLNCX-R ACC TAC AGG TGG GGT CTT TCA TTC CC pLXSN-F CTT GAA CCT CCT CGT TCG ACpLXSN-R GTT GCT GAC TAA TTG AGA TG pSHUTTLE-CMV-F GGT CTA TA T AAG CAG AGG TG pSHUTTLE-CMV-R GTG GTA TGG CTG A TT A TG A TC AG MalE Primer GGT CGT CAG ACT GTC GA T GAA GCC Invitrogen中国使用的测序载体和引物Primer of V ectorV ector Primer(F) Primer(R) pACT T7 T3pACT2 GAL4 AD pACT2-RpAS2-1 GAL4 BD pAS2-1.RpB42AD pB42ADF pB42ADRpBACPAK8 BAC1 BAC2pBK-CMV T7 T3PBS(SK/KS)/M13- M13F/T7 T3/M13RpBV220 PBV220F PBV220RpCAMBIA 1301(1300) P1 P2pCAMBIA 2300 M13R(-48) M13F(-47)PCANTB5E S1/M13R S6pCA T3-enhancer RVP3 此载体无反向引物pcDNA3.0 CMV-F/T7 SP6/BGHpcDNA3.1 T7 BGHpcDNA4 T7/CMV-F BGHpcDNA6 T7/CMV-F(J21025在T7前面）BGHpcDNAII T7 SP6pCE2.1 M13F M13RpCEP4 pCEP-F EBV-RpCF-T M13F M13RpCI T7（17Base）此载体无反向引物pCI-neo T7（17Base）T3pCMS-EGFP T7 T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5 pCMV5F pCMV5RpCMV5-Flag pCMV5F pCMV5RpCMV-MYC/HA pCMV-F pCMV-RpCMV-Sport M13F/T7 SP6/M13R pCMV-Tag(KAN+) T3 T7pCR2.1-TOPO M13F/T7 M13R pCR3.1 T7 BGH pCS2 SP6 T7 pDonar M13F M13R pDONR221 M13F M13R pDrive T7 SP6 pDRIveR(KAN+) T7 SP6pDsRED1-C1 pDsRED-ex-C1-F pEGFP-N-3’(距离很近,一般不用)pDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3’pDSRED-N1(KAN+) pEGFP-N-5’PDSRED-N-R pECFP-C pEGFP-C-5’pECFP-C-3' pECFP-N1 pEGFP-N-5’pEGFP-N-3’pEF/myc/ER pEF-F pCDNA3.1R pEGFP-C(KAN+) pEGFP-C-5’pEGFP-C-3' pEGFP-N(KAN+) pEGFP-N-5’pEGFP-N-3’pENTR/D-TOPO(KAN+) M13F M13RpET-*(His)(KAN+) T7 T7 TerpET22(a,b,c)(AMP+) T7 T7 TerpET28,30,24,49(KAN+) T7 T7 TerpET32(a,b,c)(AMP+) T7/S.tag T7 TerpET50(amp+) T7/s.tag T7TERpET44(AMP+) T7/s.tag T7 TerpEYFP-N1 pEGFP-N-5’pEGFP-N-3’pEYFP-C1 pEGFP-C-5’pEGFP-C-3' pFLAG-CMV pFLAG-CMV -F pFLAG-CMV -R pGAD424 GAL4 AD 3'AD pGADGH GAL4 AD 3'ADpGADT7-Rec GAL4 AD/T7 3'ADpGAPZa-A a-FACTOR 3'AOXpGBKT7(Kana+) T7 3'BDpGEM-T(-Easy) M13F/T7 SP6/M13R pGEX-(*)T pGEX-4T-5’pGEX-4T-3’pGL2 GLP1 GLP2pGL3 RVP3 GLP2(RVP4)(没有特别说明用GLP2)PinpointTM V ector Pinpoint Primer SP6pIRES PIRES-F/T7 T3/PIRES-R (J40720) pIRES2-EGFP pIRES2-EGFP.P5’pIRES2-EGFP.P3’pLXSN pLXSN-F pLXSN-RpMAL-c2E MalE primer M13F(-47)pMAL-C2x P5’P3’pMAL-p2X MalE primer M13F(-47)PMD18-T M13R(-48) M13F(-47)PMD19-T M13F(-47) M13R(-48)pPIC9K(AMP+、ZEO+）5’AOX/a-factor 3’AOXpPICZa 5'AOX/PPICZa-F 3'AOX pPROEXHTA M13R(-48) 无反向引物pQE30or40 pQE30-F pQE-R:pRSET T7 T7TERpSilencer3.1-H1 hygro M13F(-47) 3.0REVpSILENCEV2.0-U6 T7 2.0REVpSK01-T M13F M13RpSK-CMV(KAN+) T7 T3pSP72 T7 SP6pSport1 SP6/M13F(-47) T7/M13RpSUPER T7 T3pTA2 M13F/T7(优先使用M13F）T3/M13RpT-Adv M13F /T7 M13RpTARGET TM pTarget.F(在T7前面）/T7 pTarget.RpTO-T7 T7 T7TERpTRC99a-c pTRC99C-F pTRC99C-R pTriPLEx2 5'pTriPLEx2 T7pTWIN-1 T7 T7TER(客户确认再用）pUC18(19)/118(119) M13F(-47) M13R(-48)pUCm-T M13F/T7 M13RpV AX1 T7 BGHpWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48) pXT7 T7 SP6pYES2 T7 pY es2.RpLEXA pLEXA-F pLEXA-RpLNCX pLNCX-F pLNCX-R pRECEIVER CMV-F 此载体无反向引物pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R pSP64/65 SP6 此载体无反向引物pSTBlue-1 T7 SP6pT7Blue(R) T7 M13F(-47)。