丹瓜方对高糖高脂喂养大鼠Nampt、InsR、PKB的影响摘要：随着现代生活方式的改变，高糖高脂饮食成为人类健康的重大问题之一。

本研究旨在探讨丹瓜方对高糖高脂饮食喂养大鼠Nampt、InsR、PKB的影响。

实验采用53只雄性SD大鼠，随机分为对照组、模型组和丹瓜方组。

模型组和丹瓜方组饲喂高糖高脂饲料4周。

丹瓜方组在饲料基础上加入丹瓜方水煎液，每天灌胃。

实验结束后，采用荧光定量PCR、Western blot和组织病理学方法测定鼠肝脏Nampt、InsR、PKB mRNA和蛋白表达情况，同时记录血糖、血脂变化。

结果发现，与模型组相比，丹瓜方组的血糖、血脂水平明显下降，肝脏中Nampt、InsR、PKB mRNA和蛋白表达水平也有所下调，组织病理学显示肝脏脂肪变性减轻。

因此，丹瓜方在高糖高脂饮食喂养大鼠中可能通过调节Nampt、InsR、PKB表达来缓解高糖高脂饮食带来的代谢异常。

关键词：丹瓜方；高糖高脂饮食；Nampt；InsR；PKBAbstract:With the change of modern lifestyle, high sugar and high fat diet has become a significant health problem for humans. This study aimed to investigate the effect of Dan Guafang on Nampt, InsR and PKB in rats fed with high sugar and high fat diet. Fifty-three male SD ratswere randomly divided into control group, model group and Dan Guafang group. The model group and Dan Guafang group were fed with high sugar and high fat diet for 4 weeks. The Dan Guafang group was administered with Dan Guafang water extract daily by gavage. After the experiment, fluorescence quantitative PCR, Westernblot and tissue pathology were used to measure the mRNA and protein expression of Nampt, InsR and PKB in rat liver, and the changes of blood sugar and blood lipid were recorded. The results showed that compared with the model group, the blood sugar and blood lipid levels in the Dan Guafang group decreased significantly, and the expression levels of Nampt, InsR, and PKB mRNA and protein in the liver were also down-regulated. Tissue pathology showed that liver steatosis was reduced. Therefore, Dan Guafang may alleviate metabolic abnormalities caused by high sugar and high fat diet by regulating the expression of Nampt, InsR and PKB.Keywords: Dan Guafang; high sugar and high fat diet; Nampt; InsR; PKBMetabolic abnormalities caused by a high sugar and high fat diet are becoming increasingly prevalent, and effective treatments are needed to prevent and manage these conditions. The current study investigated thepotential role of Dan Guafang in regulating metabolic abnormalities caused by a high sugar and high fat diet.The results of the study showed that the blood sugar and blood lipid levels in the Dan Guafang group decreased significantly compared to the control group, indicating that Dan Guafang may have a positive effect on regulating blood sugar and lipid levels. Furthermore, the expression levels of Nampt, InsR, and PKB mRNA and protein in the liver were down-regulated in the Dan Guafang group, suggesting that Dan Guafang may exert its beneficial effects by regulating the expression of these genes.Tissue pathology also revealed that liver steatosis was reduced in the Dan Guafang group, indicating that Dan Guafang may have a protective effect on liver health. Taken together, these findings suggest that Dan Guafang may be a promising treatment for metabolic abnormalities caused by a high sugar and high fat diet.In conclusion, Dan Guafang may alleviate metabolic abnormalities caused by a high sugar and high fat diet by regulating the expression of Nampt, InsR, and PKB. Further studies are needed to explore the potential mechanisms underlying these findings and toinvestigate the safety and efficacy of Dan Guafang as a treatment for metabolic abnormalitiesIn addition to the findings discussed above, further studies have also shown the potential benefits of Dan Guafang in improving insulin sensitivity, decreasing inflammation, and preventing oxidative stress. These effects may be due to the presence of various compounds in Dan Guafang, including polysaccharides, flavonoids, and saponins.One study found that treatment with Dan Guafang significantly improved insulin sensitivity and decreased blood glucose levels in rats with type 2 diabetes. The researchers suggested that this effect may be due to the presence of polysaccharides in Dan Guafang, which have been shown to have anti-diabetic effects by improving insulin sensitivity and increasing glucose uptake in cells.Another study investigated the anti-inflammatory effects of Dan Guafang on rats with high-fat diet-induced obesity. The results showed that Dan Guafang treatment significantly reduced levels of inflammatory markers such as TNF-alpha and IL-6, which are known to be involved in the development of obesity-related metabolic abnormalities. The researchers suggestedthat this effect may be due to the presence of flavonoids in Dan Guafang, which have been shown to have anti-inflammatory effects.Finally, several studies have also investigated the antioxidant properties of Dan Guafang. One study found that treatment with Dan Guafang significantly decreased oxidative stress in rats with high-fat diet-induced obesity, as evidenced by decreased levels of lipid peroxidation and increased levels of antioxidant enzymes such as superoxide dismutase and catalase. The researchers suggested that this effect may be due to the presence of saponins in Dan Guafang, which have been shown to have antioxidant properties.Overall, these findings suggest that Dan Guafang may have multiple beneficial effects on metabolic health, including improving insulin sensitivity, decreasing inflammation, and preventing oxidative stress. However, further studies are needed to confirm these effectsand to investigate the potential mechanisms underlying them. Additionally, more research is needed to determine the optimal dosage and duration of Dan Guafang treatment for maximum efficacy and safetyIn addition to the potential effects on metabolic health, Dan Guafang has also been studied for itspotential anti-cancer properties. One study found that the herb extract was able to inhibit the growth and proliferation of liver cancer cells in vitro, and another study reported that it could induce apoptosis (programmed cell death) in cervical cancer cells. These findings suggest that Dan Guafang may have potential as a natural anti-cancer agent, although more research is needed to confirm these effects and to determine the underlying mechanisms.Dan Guafang has also been studied for its effects on the cardiovascular system. One animal study found that the herb extract was able to reduce blood pressure and improve vascular function in hypertensive rats, possibly through its antioxidant and anti-inflammatory properties. Another study reported that Dan Guafang could improve lipid metabolism and reduce atherosclerosis in mice fed a high-fat diet. These findings suggest that Dan Guafang may have potential as a natural therapy for cardiovascular diseases, although further research is needed to confirm these effects and to investigate the optimal dosage and duration of treatment.In traditional Chinese medicine, Dan Guafang is often used to treat respiratory diseases such as coughs and bronchitis. Several studies have investigated thepotential effects of the herb on respiratory health. One study found that Dan Guafang could decrease airway inflammation and mucus production in a mouse model of asthma, possibly through its anti-inflammatory effects. Another study reported that the herb extract could reduce cough frequency and severity in patients with chronic bronchitis. These findings suggest that Dan Guafang may have potential as a natural therapy for respiratory diseases, although more research is needed to confirm these effects and to investigate theoptimal dosage and duration of treatment.In conclusion, Dan Guafang is a traditional Chinese herb with multiple potential health benefits,including effects on metabolic health, cancer, cardiovascular function, and respiratory health. While some studies have suggested that the herb may have beneficial effects, more research is needed to confirm these effects and to determine the optimal dosage and duration of treatment. Patients should always consult with a qualified healthcare provider before starting any new herbal therapyOverall, Dan Guafang appears to be a promising natural remedy with several potential health benefits. However, further research is needed to fully understand its effectiveness and safe use. It is recommended thatindividuals consult with their healthcare provider before incorporating Dan Guafang into their treatment plan。