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Chapter3 Results and Discussions
Fig3-1 glucose’s standard curve
0.3 0.25 y = 0.0058x - 0.0136 2 R = 0.9919
absorbance
0.2 0.15 0.1 0.05 0 -0.05 0 10 20 30 40 50 60 concentraction of glucose
2.52 2.5
4.31 2.5
4.59 2.5
4.87 2.5
mass of 5 pumpkin powder (g)
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Fig.3-2 Effect of the ratio of material to solvent on extraction rate
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Table3.1 Effect of the ratio of material to solvent on extraction rate
ratio of 1:10 material to solvent absorbanc e 0.027
1:20
1:30
1:40
1:50
1:60
0.014 4.76
0.023 6.31
0.049 10.79
0.053 11.48
0.057 12.17
concentrat 7.00 ion of polysacch aride (μg /mL) extraction rate﹪ 1.40
1.90 2.5
6
extraction rate
5 4 3 2 1 0 1:10 1:20 1:30 1:40 1:50 ratio of material to solvent 2:00
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Table3.2 Effect of time on extraction rate
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K2 30.69 K3 49.5138.70 44.4641.26 34.19
k1
12.827
11.840 12.900
10.230 14.820 16.503 6.273 2.980
14.410 13.753
11.397 3.013
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k2
k3 R

From results of orthogonal experiment, we were told that the optimal ratio of complex enzymes was: the cellulose is 1%，pactinase is 1.5%，papain is 1%. In the above optimal conditions， extraction rate is 19.32%.
1.00 2.5
1.63 2.5
2.52 2.5
3.84 2.5
5.21 2.5
5.56 2.5
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Fig.3-3 Effect of time on extraction rate
6
extraction rate
5 4 3 2 1 0 0.5h 1h 2h 3h extraction time 4h 5h
4h 0.072
14.76
5.90
ratio of material to solvent
1:40
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3.5 Determination of the optimal ratio of complex enzyme
Orthogonal Table factors serial number A cellulose (%) 1(0.3) 1(0.3) 1(0.3) B pectinase (%) 1(0.5) 2(1) 3(1.5) C papain (%) 1(1) 2(1.5) 3(2) extraction rate(%) 12.74 13.71 13.12
Conclusions
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Chapter1 Introduction Recently, it was found pumpkin is not only nutritious, but also has a variety of therapeutic health effects, the research on extraction of polysaccharides from pumpkin is provided to improve its applications .
1 2 3
4
5 6
2(0.5)
2(0.5) 2(0.5)
1(0.5)
2(1) 3(1.5)
2(1.5)
3(2) 1(1)
9.68
8.07 13.56
7
8 9 K1
3(1)
3(1) 3(1) 38.48
1(0.5)
2(1) 3(1.5) 35.52
3(2)
1(1) 2(1.5) 43.23
13.86
18.08 19.04
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3.4 Determination of pumpkin polysaccharide in the
optimum condition
extraction time absorbance concentration of polysaccharide (μg/mL) extraction rate ﹪
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Conclusions
1. 1:40 was chosen as the ratio of material to solvent,4 hours was chosen as the extraction time. TEXT TEXT TEXT TEXT 2. Optimal ratio of complex enzymes: the cellulose is 1%,pactinase is 1.5% and papain is 1% . 3. Enzymes extraction is superior to hot water extraction.
extraction time absorbance concentration of pumpkin polysacchari de (μg/mL) extraction rate﹪ mass of pumpkin powder (g) 0.5h 0.001 2.51 1h 0.010 4.07 2h 0.023 6.31 3h 0.042 9.59 4h 0.062 13.03 5h 0.067 13.90
Extraction of pumpkin polysaccharide from the pumpkin powder
南瓜粉中南瓜多糖的提取
指导教师：张华 姓 名：张成林
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Contents
Introduction Test method Results and discussions
1
2
3
0.5
1.0
1.0
1.5
1.5
2.0
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2.4 Determination of the rate of pumpkin polysaccharide
Extraction rate of polysaccharides (%)=(concentration of polysaccharides ×dilution rate×10-6)/weight of sample×100%
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Fig.3-7 Comparison between hot water extraction and complex enzymes extraction

25
extraction rate
20 15 10 5 0 1 2 two different ways of extraction hot water extraction enzymes extraction
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Chapter2 Test method
2.1 Effect of the ratio of material to solvent on extraction rate
the ratio of 1：10 materia l to solvent Temperatu re (℃) Time(hour) 70 1:20 1:30 1:40 1:50 1:60
70
70
70
70
70
2
2
2
2
2
2
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2.2 Effect of time on the extraction rate
the ratio of materia l to solvent
1:30
1:30
1:30
1:30
1:30
1:30
Temperatu re (℃)
Time (hour)
70
70
70
70
70
70
0.5
1
2
3
4
5
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2.3 Determination of complex enzymes ratio
Level
A cellulase (%) 0.3