如何测定荧光量子效率首先要有一个已知量子产率的标准物质，一般用硫酸奎宁。
分别做标准物质和未知样品的吸收和荧光光谱。
Φu=Φs(Yu/Ys)(As/Au)Φu——待求物质的荧光量子产率；Φs——标准物质的荧光量子产率；Yu——待求物质的荧光积分面积；Ys——标准物质的荧光积分面积；Au——待求物质的荧光激发波长处的吸收值；As——标准物质的荧光激发波长处的吸收值。
测定激发光强度和荧光强度就可以了知道吧？如果已知一标准物质的量子产率，可以配置相同浓度的标准和待测物质，在相同的测量条件下，测量两者的荧光强度.未知物质的荧光量子产率=F未知/F标准*标准的量子产率。
量子效率是指发射的光子数Nf与激发时吸收的光子数NX之比,即量子效率=Nf/NX我们知道,一般的发光总有一定的能量损失,激发光子的能量总是大于发射光子的能量.所以,量子效率通常都是小于100%.在实际测量时我们不好去测量发射的光子数Nf与激发时吸收的光子数NX,而通常用用下面的公式求算:量子效率=发射峰的最大发光强度/激发峰的最大发光强度但是注意根据待测物的激发和发射波长的范围，选择不同激发和发射波长的基准物。
请问在有机电致发光中关于量子效率中的外量子效率是如何计算的公式是什么样子的exp(eV/kT)=aP2+bP+c(1)其中V为光生电压，k为玻尔兹曼常数，T为绝对温度，e为电子电荷，a，b，c 为常数。
Additionally,α-NPN thin film emits bright blue fluorescence(461 nm) 1.-NPD(5.5%). efficiency of 8.5%,higher than that of αwith a quantum该化合物可发明亮的蓝色荧光(461 nm),积分球测定其薄膜荧光量子效率为8.5%,比α-NPD 的5.5%高。
收藏指正emission properties were studied and their photolysisTheir fluorescence2.kinetics was investigated indifferent media by means of steady statephotolysis method.较低，光谱特性，发现甲氧基苯甲酰亚甲基光产碱剂的荧光量子效率考察了其荧光而萘甲酰亚甲基光产碱剂较高。
收藏指正of oxazine 1 perohlorate in 1, 2-efficiency quantum The fluorescence3.dichloro-ethane was measured with photoacoustic speetroscopy in whichthe fluorescence quenching wag used.荧光高氯酸盐的二氯乙烷溶液进行)嗪1利用光声光谱技术,采取猝灭的方法对(口恶收藏指正量子效率的测量。
for theefficiencyquantum The experimental results indicate that the 4.μm than that at 0.80 μm, emission at 1.5 μm is higher upon excitation at 0.98owing to the much weaker excited state absorption (ESA) compared withthose of excitation at 0.80 μm. 因而其激发下小得多, ,激发态吸收较0 .80μm研究表明:由于在0 .98μm激发下;收藏指正也比0 .80μm激发下高得多荧光1.5μm发射量子效率-property of coumarin derivative CND_(500) with βFluorescence5.-CD) in aqueous solution and CND_(500) with ethyl alcohol, cyclodextrin (βmethod. It n-saf Triton X-100 micelle solution are examined by fluorescence fluorescence is observed that the peak, relative intensity (F), polarization(P), relative quantum yield ((?)用荧光法研究了香豆素衍生物CND_(500)在β-CD水溶液中和在乙醇及非离子表面活性剂(n-Saf)Triton X-100等胶束溶液中的荧光特性,发现CND_(500)在β-CD水溶液中的荧光强度(F),荧光偏振度(P)、相对量子效率((?)收藏指正Abstract: Polyphenylacetylenes were synthesized by ［Rn(nbd)Cl］2 and 6.WCl6/Ph4Sn catalyst systems in N2 at room temperature.Polymerswithhigh yield and high molecular weight were obtained.Their structures werecharacterized by UV,IR,NMR and GPC,respectively.The fluorescence of thepolymer solution with different concentration was investigated using variable excitation wavelength from UV to visible region.The relation between fluorescence and polymer structure was studied.The resultsshowed that the molecular chain structure has great influence on fluorescence of the polymers.The emitting peak wavelength offluorescence for the polymer of high stereoregularity is independent ofsolution concentration and excitation wavelength.The emitting peak wavelength of fluorescence for the polymer with disordered molecularchain arrangement varies with changing the solution concentration and excitation wavelength,and different emission sites in molecular chains andlow emitting yield are shown.The measurement and analysis of fluorescence spectroscopy of polyphenylacetylenes may provide someuseful information to judge the molecular chain arrangement in someconjugated polymers.文摘:使用有机金属铑、金属钨/四苯基锡催化剂体系聚合苯乙炔，分别获得了高产等分别对聚合物结构进行了GPC，NMR，IR，UV使用.率和高分子量的聚苯乙炔．表征.采用不同波长的激发光对聚合物荧光性能进行研究，详细分析了聚合物结构与荧光性能之间的关系.研究结果发现，不同聚合物结构对聚合物荧光性能产生很大影响，规整性高的聚合物，将有较高的发光量子效率；聚合物规整性差，将可能导致多个荧光发光结构点，其荧光强度降低.聚合物荧光光谱研究将对某些共轭聚合物结构的规整性分析提供一些有用的信息.收藏指正The rate constant and the efficiency of main energy trasfer process were 7.calculated from fluorescence lifetime, phosphorescence lifetime,quantumyield and time-resolved spectra of I3MK and I3MK-Tb(Ⅲ)stsyem. Accordingly,the dynamics of the energy-transfer process in this system was analysed. A modification of the perrin formula was proposed and the mechanism of the energy transfer of the complex system was disscussed.从I3MK和I3MK－Tb（Ⅲ）体系的荧光寿命、磷光寿命、量子产率，时间分辨光谱等，计算了各主要能量传递过程的速率常数和能量传递效率，进行了该体系能量传递过程的动态分析，提出了Perrin公式的修正参数，讨论了I3MK－Tb（Ⅲ）体系的能量传递机理．收藏指正The results showed that with the increasing water8.stress,Fv/Fm,Fv/Fo,Yield,qP and Photo values decreased,whileqN,whichreflects the protective mechanism of light energy,increased significantly.研究结果表明,叶片的叶绿素荧光参数PSII原初光能转换效率(Fv/Fm)、PSII潜在活性(Fv/Fo)、光合量子产额(Yield)、光化学淬灭系数(qP)和净光合速率(Photo)值随水分胁迫强度增强而呈下降的趋势;收藏指正Either of the two stresses increased minimum chlorophyll9.florescence(F0),and decreased maximum chlorophyllfluorescence(Fm),PSⅡprimary chemicalefficiency(Fv/Fm)and PSⅡpotential activity(Fv/F0),butdid not significantly result in variation in quantum yield of PSⅡlinearelectron(ΦPSⅡ) relative to the un-stressed control. POD activity,MDA andPro contents were significantly raised by drought or salinity.干旱或盐胁迫引起初始荧光(F0)上升,最大荧光(Fm)、PSⅡ原初光能转化效率(Fv/Fm)和PSⅡ潜在活性(Fv/F0)显著下降,PSⅡ实际光化学量子产量(ΦPSⅡ)略有降低,而POD活性、MDA和Pro含量显著提高。