Inhibition of cell metabolism Production of viruses compromised
Disruption of nucleic acid synthbrane antigenicity
CELL DEATH
DNA fragmentation due to Mycoplasmal nucleases NOT apoptosis
Program I-013 + DNA
57.7% GFP+ 转染效率由57.7%下降至23.0%！
23.0% GFP+
多家期刊要求发表文章前进行支原体检测
当你发表文章后，同行慕名，向实验室 求赠细胞系时。。。
Biotool支原体快速检测试剂盒
Biotool支原体快速检测试剂盒
最终反应颜色深浅程度与支原体污染程度呈正相关！
支原体污染严重影响实验结果的可靠性、重现性和一致性！
支原体污染影响肿瘤细胞在裸鼠体内的成瘤性
支原体污染降低转染效率
HeLa cells uninfected
Program I-013 no DNA
HeLa cells infected with Mycoplasma fermantans
no program + DNA
支原体污染影响细胞多种生理参数
Increased sensitivity Inhibition of cell growth to inducers of apoptosis
Alteration of DNA transfection efficiency
Chromosomal aberrations
Price comparison
Brand Biotool Lonza Sigma R& D Invivogen
Price/Test 0.88 USD 7.25 USD 10.29 USD 1.25 USD 1.35 USD