Vector
extrachromosomal state, most of which exist as
Forms of Plasmid DNA •Supercoiled, native form,
c ovalently c lose
d c ircles
超螺旋型，即共价闭合环
质粒的构型－电镜照片
Electrophoresis of plasmid DNA in agarose gels
1.
as
confer on their host cells
天然质粒
Zhu, et al, AAC,
2009,
A large plasmid isolated from Klebsiella pneumoniae
Chloramphenicol
抗生素抗性
¾
1.The ColE1 origin of replication is
Regulation of replication of
-445 -555
on the Rop protein
Low and High Copy Number
Replication of ColE1 also depends
stability of plasmids
离和分析－
PLASMID 56(2006):167-178
什么是军团菌？
z革兰氏阴性杆菌
z条件致病菌，可通过气溶胶传播，引致非典型肺炎——军团病。

z广泛存在于水体、土壤和寄生于自由生活的单细胞原生动物中，
z可在原生动物体内存活并繁殖，在淡水环境下有很强的抗逆能力。

如何研究军团菌与原生动物的关系
ColE1质
粒
复
制
起
始
的
控
制。