GAA
GAGTACTC AAAAGTACTTTT
切割率%
2 hr 20 hr
0
0
10
10
10
10
25
90
25
>90
10
>90
>90
>90
0
0
0
25
0
>90
0
0
0
25
0
50
75
>90
0
0
10
10
>90
>90
>90
>90
0
0
0
0
10
25
0
10
10
10
0
0
50
>90
0
0
10
50
10
75
10
25
75
75
Sma I Spe I Sph I Stu I Xba I Xho I Xma I
75
GGGTACCC GGGGTACCCC CGGGGTACCCCG
0
0
>90
>90
>90
>90
GACGCGTC CGACGCGTCG
0
0
25
50
CCCATGGG CATGCCATGGCATG
0
0
50
75
Nde I Nhe I
CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC
CTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG
CCTCGAGG CCCTCGAGGG CCGCTCGAGCGG
CCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA
寡核苷酸序列
GGTCGACC CGGTCGACCG CCGGTCGACCGG
0
0
25
75
50
>90
>90
>90
切割率%
2 hr 20 hr
0
0
0
0
0
0
0
0
>90
>90
>90
>90
>90
>90
>90
>90
>90
>90
Ava I BamH I Bgl II BssH II BstE II BstX I Cla I
EcoR I Hae III Hind III Kpn I Mlu I Nco I
酶 Acc I Afl III Asc I
CCCGGG CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
GACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG
GGCATGCC CATGCATGCATG ACATGCATGCATGT
AAGGCCTT GAAGGCCTTC AAAAGGCCTTTT
CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA
50
>90
>90
>90
>90
>90
CGGATCCG CGGGATCCCG CGCGGATCCGCG
10
25
>90
>90
>90
>90
CAGATCTG GAAGATCTTC GGAAGATCTTCC
0
0
75
>90
25
>90
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
CCGATCGG ATCGATCGAT TCGCGATCGCGA
CGAGCTCG
GCCGCGGC TCCCCGCGGGGA
GTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCG
G ACGCGTCGACGTCGGCCATAGCGGCCGCG
CACATGTG CCACATGTGG CCCACATGTGGG
GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA
0
10
0
10
10
50
>90
>90
10
>90
10
>90
0
50
0
50
0
0
0
25
10
50
>90
>90
>90
>90
>90
>90
0
0
>90
>90
75
>90
75
>90
0
0
10
25
10
75
GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG
0
0
0
0
0
0
0
0
75
>90
75
>90
0
0
10
25
10
50
0
0
0
0
50
>90
GGGT(A/T)ACCC
0
10
AACTGCAGAACCAATGCATTGG
0
0
AAAACTGCAGCCAATGCATTGGAA
25
50
CTGCAGAACCAATGCATTGGATGCAT
25
>90
CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG
0
0
0
0
>90
>90
50
50
GGAATTCC CGGAATTCCG CCGGAATTCCGG
>90
>90
>90
>90
>90
>90
GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA
>90
>90
>90
>9TTG CCAAGCTTGG CCCAAGCTTGGG
0
0
0
0
10
酶 Not I
Nsi I Pac I Pme I
Pst I
Pvu I Sac I Sac II Sal I
Sca I
寡核苷酸序列
TTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCAAAAGGAAAA
TGCATGCATGCA CCAATGCATTGGTTCTGCAGTT
TTAATTAA GTTAATTAAC CCTTAATTAAGG
GTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG
GCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT