645 649 650 650 696 650 650
660 FL-4 666 668 690 719 774 774 FL-4 FL-4 FL-3 APC APC APC APC/Cy5.5 APC/Cy5.5 APC/Cy7 APC/Cy7 APC/Cy7 APC/Cy7 APC/Cy5.5 APC APC APC APC APC/Cy5.5 APC/Cy5.5 APC/Cy7 APC/Cy7 APC APC APC APC/Cy5.5 APC/Cy5.5 APC/Cy7 APC/Cy7 FL-4 FL-4 FL-4 FL-4 FL-4 FL-5 FL-5 FL-7 FL-7 FL-7 FL-7 FL-7 FL-8 FL-8 APC APC APC APC/Cy5.5 PE/Cy5 APC/Cy7 APC/Cy7 FL-8 FL-8 FL-8 FL-4 FL-4 FL-9 FL-9 APC APC APC APC-Cy5.5 APC-Cy5.5 APC-Cy7 APC-Cy7 FL-4 FL-4 FL-4 FL-3 Channel 6 Channel 6 Channel 6 Channel 6 Channel 6
496, 565 514 496, 565 482 496, 565 482 496, 565 496, 565
488 488, 514 488 488 488 488 488 488 595, 633, 635, 647 633, 635 595, 633, 635, 647 595, 633, 635, 647 633, 635 595, 633, 635, 647 595, 633, 635, 647
546
647 FL-3 FL-3 FL-3 PerCP/Cy5.5 PerCP/Cy5.5
PerCP/Cy5.5 PerCP/Cy5.5
FL-3 FL-3
FL-3 FL-3
FL-4 FL-4
PE/Cy5 PE/Cy5
FL-4 FL-4
PM-2 PM-1
PE-Cy5 PE-TR
FL-3 FL-2
Channel 5 Channel 5
® ® ®
377 401 410 458 400 400 495 493
360, 405, 407 360, 405, 407 360, 405, 407 405, 407, 457 405, 407 360, 405, 407 488 488
420 421 455 489 550 551 519 525
Fluorochromes for Flow Cytometry and Microscopy
Excitation Laser Lines (nm) BD BD BD BD FAC- BD FACSVanFACFACFACSCanto™ SCalibur™ tage™SE Scan™ SCanto™ II Beckman Coulter® EPICS® XL™/ XL-MCL™ Beckman CYTOPEIA Dako CyAn™ Coulter® FC Dako MoFlo® Guava EasyCyte iCyt Reflection® inFlux™ ADP 500 Amnis ImageStream®
575 FL-2 566 670 675 690 690 613 774 FL-2 FL-3 FL-3 FL-3 FL-3 FL-2 FL-3 FL-3 FL-2 FL-2 FL-3 FL-3 FL-3 FL-3 PerCP/Cy5.5 PerCP/Cy5.5 PE PE/Cy7 PE/Cy7 PE PE PE/Cy5 PE/Cy5 PerCP/ Cy5.5 PerCP/ Cy5.5 PE PE PE PE PE/Cy5 PE/Cy5 PerCP/Cy5.5 PerCP/Cy5.5 PE PE/Cy7 PE PE PE/Cy5 PE/Cy5 PerCP/ Cy5.5 PerCP/ Cy5.5 PE PE/Cy7 FL-2 FL-2 FL-4 FL-4 FL-4 FL-4 FL-3 FL-2 FL-2 FL-4 FL-4 FL-4 FL-4 FL-3 FL-5 FL-4 FL-4 FL-6 FL-6 FL-6 FL-6 FL-5 PE PE PE/Cy5 PE/Cy5 PE/Cy5 PE/Cy5 PE/Texas Red PE/Cy7 FL-3 FL-5 PM-4 FL-4 FL-2 FL-2 FL-4 PM-1 PM-1 PM-2 PM-2 PM-2 PM-2 PE PE PE-Cy5 PE-Cy5 PE-Cy5.5 PE-Cy5.5 PE-TR PE-Cy7 FL-3 FL-2 FL-2 FL-3 FL-3 FL-3 FL-3 Channel 4 Channel 4 Channel 6 Channel 6 Channel 6 Channel 6 Channel 5
FL-1 FL-1 FL-1 FL-1 FL-2 FL-2 FL-3 FL-3
DAPI DAPI DAPI DAPI Pac. Orange Pac. Orange FITC FITC
FL-6 FL-6 FL-6 FL-6 FL-7 FL-7 FL-1 FL-1 PM-3 PM-3
DAPI/PacBlue DAPI/PacBlue DAPI/PacBlue DAPI/PacBlue Pac. Orange Pac. Orange FITC FITC FL-1 FL-1
305, 540
325, 360, 488
620
FL-3
Trademarks: BD FACScan™ , BD FACSCaliber™, BD FACSVantage™ SE, BD FACSCanto™, BD™ LSRII, BD FACSAria™ are trademarks of Becton, Dickinson and Company. Coulter® EPICS® XL™/XL-MCL™ are registered trademarks of Beckman Coulter, Inc. inFlux™ is a trademark of Cytopeia, Inc. iCyt Reflection® is a registered trademark of iCyt Mission Technology, Inc. CyAn™ ADP and MoFlo™ are trademarks of Dako Denmark A/S. C6™ is a trademark of Accuri. Image Stream® is a registered trademark of Amnis Corporation. Cascade Blue®, Alexa Fluor® , Texas Red® are registered trademarks of Molecular Probes, Inc. Pacific Blue™ is a trademark of Molecular Probes, Inc. * These fluorochromes can be used as single color configurations or multi-colored configurations depending on instruments, available lasers and filters, and appropriate color compensation settings. ** These instruments are provided as general information. User must check instrument capability and choose appropriate setting.
359
325, 360, 405, 407 488
461
DAPI PerCP/ Cy5.5 PerCP/ Cy5.5
DAPI
DAPI PerCP/ Cy5.5 PerCP/
DAPI/PacBlue
Channel 2
DAPI 7-AAD (7aminoactinomycin D) PI (Propidium Iodide)
Channel 2 Channel 2 Channel 2 Channel 2
Cascade Blue® Alexa Fluor® 405 Pacific Blue™ AmCyan Cascade Yellow™ Pacific Orange™
Channel 3 Channel 3
Alexa Fluor® 488 FITC (fluorescein) PE (RPhycoerythrin) Cy3 PE/Cy5 PerCP PE/Cy5.5 PerCP-Cy5.5 PE/Texas Red® PE/Cy7 APC (allophycocyanin) Cy5 Alexa Fluor® 647 APC/Cy5.5 Alexa Fluor® 700 APC/Cy7 APC/Alexa Fluor® 750 Viability Probe
Fluorochrome
Excitation Max (nm)
Emission Max (nm)
BD™
LSR II
BD FACSAria™
Accuri C6™ Flow Cytometer
Fluorochrome
Cascade Blue® Alexa Fluor® 405 Pacific Blue™ AmCyan Cascade Yellow™ Pacific Orange™ Alexa Fluor 488 FITC (fluorescein) PE (RPhycoerythrin) Cy3 PE/Cy5 PerCP PE/Cy5.5 PerCP-Cy5.5 PE/Texas Red® PE/Cy7 APC (allophycocyanin) Cy5 Alexa Fluor 647 APC/Cy5.5 Alexa Fluor 700 APC/Cy7 APC/Alexa Fluor® 750 Viability Probe DAPI 7-AAD (7aminoactinomycin D) PI (Propidium Iodide)