Angiogenesis Sprouting or Non-sprouting of cells from mature endothelial cells of the vessel wall
Vasculogenesis Formation of blood vessels by differentiation from (hem)angioblasts
• Dan Meng, M.D., Associate Professor Department of Physiology & Pathophysiology
Fudan University
Room 212, West 7 th Building, 130 Dongan Road
Shanghai 200032, China.
Basement Membrane
Endothelium
Sprout Elongation
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Angiogenesis - Capillary Morphogenesis
VEGF
Smooth Muscle Cells
Basement Membrane
Endothelium
New Lumen Formation
Several German pathologists observe that some human tumours are highly vascularised, leading to the
suggestion that the new vessels have an important pathogenic role in the cancer process1
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Angiogenesis - Vascular Maturation
VEGF
Smooth Muscle Cells
Basement Membrane
Endothelium
SMC, per课件
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Angiogenesis - Vascular Maturation
4- Angiogenesis-related diseases
5- Anti-angiogenesis therapies and therapeutic Angiogenesis
6- Angiogenesis assay model
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Angiogenesis and vasculogenesis
Smooth Muscle Cells
Basement Membrane
Endothelium
Endothelial cell-cell junctions
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Non-Sprouting Angiogenesis (Intussuseptive angiogenesis)
Landmark publication: Judah Folkman proposes tumour growth is angiogenesis-dependent2
Discovery by Napoleone Ferrara and colleagues of possibly the most important
ANGIOGENESIS
Dan Meng, MD
Department of Physiology and Pathophysiology, Shanghai Medical College Fudan University
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ConCtoantcatcttoto mmee
• 上海市东安路130号西7号楼212室 复旦大学上海医学院生理与病理生理学系 Tel:021-54237392 Email:dmeng@
Tel: 86-21-54237392
Email:dmeng@
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Outline
1- Angiogenesis and vasculogenesis
2- Important factors, receptors and signaling pathways
3- Tumor angiogenesis
VEGF
Smooth Muscle Cells
Basement Membrane
Endothelium
Nascent Vascular Sprouts
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Angiogenesis - Endothelial Cell Proliferation
VEGF
Smooth Muscle Cells
angiogenic factor, VEGF
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1Ferrara. Nat Rev Cancer 2002 6 2Folkman. NEJM 1971
Structure of vessels and capillaries
Small artery:
Capillary: endothelial cell, basal lamina, pericytes
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Angiogenesis - Basement Membrane Breakdown
Angiogenic Stimulus (VEGF)
Smooth Muscle Cells
Basement Membrane
Endothelium
Proteases
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Angiogenesis - Endothelial Cell Migration
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Angiogenesis: Sprouting angiogenesis
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Angiogenesis: Sprouting angiogenesis
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Mouse cornea: wounding induces angiogenesis, chemotactic response to angiogenic factors
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Angiogenesis and vasculogenesis
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Key landmarks in understanding angiogenesis
1787
Angiogenesis first described by Dr John Hunter
1990s
1800 1971 1989