pET-32b(+)编号 载体名称北京华越洋生物VECT5030 pET-‐32b(+)pET32b载体基本信息别名: pET32b, p et 32b质粒类型: 大肠杆菌蛋白表达表达水平: 高克隆方法: 多克隆位点，限制性内切酶载体大小: 5899bp5' 测序引物: T7或者Trx-‐F5' 测序引物序列: T7: 5'-‐TAATACGACTCACTATAGGG-‐3';Trx-‐F: 5' T TCCTCGACGCTAACCTG 3' 载体标签: thioredoxin (N端); H is (中间和C端) 载体抗性: Ampicillin备注: Production of soluble, active target proteins; N-‐term thrombin cleavage s ite;Nterm e nterokinase c leavage s ite; a,b,c v ary b y M CS稳定性: 瞬时表达 Transient组成型: 组成型 Constitutive病毒/非病毒: 非病毒pET32b载体质粒图谱和多克隆位点信息pET32b载体简介The pET-‐32a-‐c series is designed for cloning and high-‐level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection and purification. Unique sites are shown on the circle map. Note that t he s equence i s n umbered b y t he p BR322 c onvention, s o t he T7 e xpression r egion i s reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-‐stranded DNA that corresponds to the coding strand. Therefore, single-‐stranded sequencing should be performed u sing t he T7 t erminator p rimer .pET32b载体序列ORIGIN1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT 181 CGACGGAGCT CGAATTCGGA TCCGATATCG CCATGGCCTT GTCGTCGTCG TCGGTACCCA 241 GATCTGGGCT GTCCATGTGC TGGCGTTCGA ATTTAGCAGC AGCGGTTTCT TTCATACCAG 301 AACCGCGTGG CACCAGACCA GAAGAATGAT GATGATGATG GTGCATATGG CCAGAACCAG 361 AACCGGCCAG GTTAGCGTCG AGGAACTCTT TCAACTGACC TTTAGACAGT GCACCCACTT 421 TGGTTGCCGC CACTTCACCG TTTTTGAACA GCAGCAGAGT CGGGATACCA CGGATGCCAT 481 ATTTCGGCGC AGTGCCAGGG TTTTGATCGA TGTTCAGTTT TGCAACGGTC AGTTTGCCCT 541 GATATTCGTC AGCGATTTCA TCCAGAATCG GGGCGATCAT TTTGCACGGA CCGCACCACT 601 CTGCCCAGAA ATCGACGAGG ATCGCCCCGT CCGCTTTGAG TACATCCGTG TCAAAACTGT 661 CGTCAGTCAG GTGAATAATT TTATCGCTCA TATGTATATC TCCTTCTTAA AGTTAAACAA 721 AATTATTTCT AGAGGGGAAT TGTTATCCGC TCACAATTCC CCTATAGTGA GTCGTATTAA 781 TTTCGCGGGA TCGAGATCGA TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC 841 CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG 901 GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT 961 GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT 1021 CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG 1081 TCGAGATCCC GGACACCATC GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC 1141 CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG 1201 AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT 1261 CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC 1321 GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC 1381 TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG 1441 GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG 1501 TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC 1561 AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT 1621 CTGACCAGAC ACCCATCAAC AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG 1681 TGGAGCATCT GGTCGCATTG GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT 1741 CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC1801 AGCCGATAGC GGAACGGGAA GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC 1861 AAATGCTGAA TGAGGGCATC GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC 1921 TGGGCGCAAT GCGCGCCATT ACCGAGTCCG GGCTGCGCGT TGGTGCGGAC ATCTCGGTAG 1981 TGGGATACGA CGATACCGAA GACAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC 2041 AGGATTTTCG CCTGCTGGGG CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC 2101 AGGCGGTGAA GGGCAATCAG CTGTTGCCCG TCTCACTGGT GAAAAGAAAA ACCACCCTGG 2161 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC 2221 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT AAGTTAGCTC 2281 ACTCATTAGG CACCGGGATC TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC 2341 TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG 2401 CAACTCGTAG GACAGGTGCC GGCAGCGCTC TGGGTCATTT TCGGCGAGGA CCGCTTTCGC 2461 TGGAGCGCGA CGATGATCGG CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT 2521 CAAGCCTTCG TCACTGGTCC CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC 2581 GGCATGGCGG CCCCACGGGT GCGCATGATC GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC 2641 TGGCGGGGTT GCCTTACTGG TTAGCAGAAT GAATCACCGA TACGCGAGCG AACGTGAAGC 2701 GACTGCTGCT GCAAAACGTC TGCGACCTGA GCAACAACAT GAATGGTCTT CGGTTTCCGT 2761 GTTTCGTAAA GTCTGGAAAC GCGGAAGTCA GCGCCCTGCA CCATTATGTT CCGGATCTGC 2821 ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA ACACCTACAT CTGTATTAAC GAAGCGCTGG 2881 CATTGACCCT GAGTGATTTT TCTCTGGTCC CGCCGCATCC ATACCGCCAG TTGTTTACCC 2941 TCACAACGTT CCAGTAACCG GGCATGTTCA TCATCAGTAA CCCGTATCGT GAGCATCCTC 3001 TCTCGTTTCA TCGGTATCAT TACCCCCATG AACAGAAATC CCCCTTACAC GGAGGCATCA 3061 GTGACCAAAC AGGAAAAAAC CGCCCTTAAC ATGGCCCGCT TTATCAGAAG CCAGACATTA 3121 ACGCTTCTGG AGAAACTCAA CGAGCTGGAC GCGGATGAAC AGGCAGACAT CTGTGAATCG 3181 CTTCACGACC ACGCTGATGA GCTTTACCGC AGCTGCCTCG CGCGTTTCGG TGATGACGGT 3241 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 3301 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC 3361 ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC 3421 AGATTGTACT GAGAGTGCAC CATATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 3481 AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 3541 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 3601 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 3661 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 3721 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 3781 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 3841 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 3901 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 3961 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 4021 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 4081 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 4141 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 4201 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 4261 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 4321 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 4381 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG4441 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 4501 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 4561 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 4621 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 4681 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 4741 CGTTGTTGCC ATTGCTGCAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 4801 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 4861 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 4921 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 4981 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 5041 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 5101 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 5161 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 5221 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 5281 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 5341 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 5401 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA 5461 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA 5521 AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA 5581 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA 5641 GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG 5701 TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC 5761 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC 5821 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA 5881 GGGCGCGTCC CATTCGCCA//其他大肠杆菌表达载体：pBV221 ptdTomato pET-52b(+) pAmCyanpDsRed-Express2 pBV220 pCold-GST pColdS-SUMOpCold TF pCold IV pCold III pCold IIpCold I pE-SUMO pCold-ProS2 pBAD102/D-TOPO pBAD202/D-TOPO pACYC184 pBAD/Thio-TOPO pBad/Myc-His C pBad/Myc-His B pBad/Myc-His A pBad/His C pBad/His BpBad/His A pBAD-TOPO pET-23b(+) pET-23a(+)pET-23c(+) pET-23(+) pET-12b(+) pET-12c(+)pET-12a(+) pET-11b(+) pET-11a(+) pET-11c(+)pBad24 pQE-82L pQE-81L pQE-80LpQE-32 pQE-9 pQE-16 pQE-31pQE-60 pQE-70 pQE-40 pET-51b(+)pET-50b(+) pET-49b(+) pET-48b(+) pET-47b(+)pET-26b(+) pET-32a(+) pET-21b(+) pET-22b(+)pET-14b pET-16b pET-15b pET-19bpET-20b(+) pET-21d(+) pET-21c(+) pET-21b(+)pET-21a(+) pET-24a(+) pET-24d(+) pET-25b(+) pET-27b(+) pET-28a(+) pET-30a(+) pET-42a(+) pET-43.1c(+) pET-43.1b(+) pET-43.1a(+) pET-44a(+) pET-44c(+) pET-46 EK/LIC pET-37b(+) pTrcHis2 C pTrcHis2 B pTrcHis2 A pET303/CT-His pET302/NT-His pRSET-CFP pRSET-EmGFP pRSET-BFP pGFPuvpET300/NT-DEST pET301/CT-DEST pGEM-T pBad43pGEX-4T-3 pGEX-5X-2 pBlueScript SK(+) pG-Tf2pG-KJE8 pGro7 pET-SUMO pSE380pET-17b pET102/D-TOPO pCDFDuet-1 pMAL-p5xpTf16 pET-28c(+) pBluescript II SK(+) pET-30b(+) pSUMO pProEX HTc pProEX HTb pProEX HTa pKD3 pKD13 pKD46 pTYB1pTYB2 pTWIN2 pBluescript II KS(-) pTYB12pMAL-p5e pACYCDuet-1 pEGM-11ZF(+) pEGM-7ZF(+) PinPoint Xa-3 PinPoint Xa-2 PinPoint Xa-1 pSP73pSP64 pTWIN1 pTYB11 pTXB1pET-5b(+) pBad/gIII C pBad/gIII B pBad/gIII A pET-5a(+) pMal-p4X pMal-p2G pkk223-3pkk232-8 pCYB1 pEZZ18 pBAD18pMAL-c5x pMal-p2E pMal-p2X pET-44 EK/LIC pET-43.1 EK/LIC pET-41 EK/LIC pMal-c4X pTrcHis BpET-31b(+) pET-3b(+) pET-41a(+) pGEX-3XpGEX-4T-2 pETDuet-1 pGEX-4T-1 pTrc99apET-28b(+) pET-His pALEX a,b,c pACYC177pBR322 pKD4 pKD20 pMXB10pEcoli-6xHN-GFPuv pKJE7 pRSET B pGEX-KGpGEX-2T pRSFDuet-1 pCOLADuet-1 pTrcHis C pTrcHis A pET-41b(+) pET-42b(+) pET-3a(+) pGEX-6P-3 pGEX-6P-2 pGEX-6P-1 pGEX-5X-3 pGEX-5X-1 pGEX-2TK pRSET A pMal-c2GpMal-c2E pMal-c2X pRSET C pQE-30pET-45b(+) pET-44b(+) pET-42c(+) pET-41c(+) pET-40b(+) pET-33b(+) pET-39b(+) pET-32 EK/LIC pET-32 Xa/LIC pET-32c(+) pET-32b(+) pET-30 Xa/LIC pET-30 EK/LIC pET-30c(+) pET-29c(+) pET-29b(+) pET-29a(+) pET-24c(+) pET-24b(+) pET-24(+)pET-23d(+) pET-11d(+) pBad33。