pKD4编号 名称北京华越洋VECT-‐510 pKD4pKD4载体基本信息载体名称: pKD4质粒类型: 细菌表达；基因敲除系统 高拷贝/低拷贝: -‐-‐启动子: -‐-‐克隆方法: 多克隆位点，限制性内切酶 载体大小: 3267 b p5' 测序引物及序列: Amp-‐R: A TAATACCGCGCCACATAG C3' 测序引物及序列: -‐-‐载体标签: -‐-‐载体抗性: 氨苄青霉素（Ampicillin）筛选标记: 新霉素（Neomycin）备注: -‐-‐稳定性: -‐-‐组成型: -‐-‐病毒/非病毒: -‐-‐pKD4载体质粒图谱和多克隆位点信息pKD4载体简介pKD4载体是RED系统敲除载体，复制子是R6K y o ri，质粒大小是3267bp，带有Ampicillin(Amp)和Kanamycin（Kan）抗性，可转化进大肠杆菌DH5α，通过抗生素抗性筛选大量复制扩增，培养条件是LB，30℃。
pKD4载体序列ORIGIN1 AGATTGCAGC ATTACACGTC TTGAGCGATT GTGTAGGCTG GAGCTGCTTC GAAGTTCCTA 61 TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCAA GATCCCCTCA CGCTGCCGCA 121 AGCACTCAGG GCGCAAGGGC TGCTAAAGGA AGCGGAACAC GTAGAAAGCC AGTCCGCAGA 181 AACGGTGCTG ACCCCGGATG AATGTCAGCT ACTGGGCTAT CTGGACAAGG GAAAACGCAA 241 GCGCAAAGAG AAAGCAGGTA GCTTGCAGTG GGCTTACATG GCGATAGCTA GACTGGGCGG 301 TTTTATGGAC AGCAAGCGAA CCGGAATTGC CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA 361 AGCCCTGCAA AGTAAACTGG ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT 421 CAAGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC 481 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 541 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 601 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT 661 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG 721 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 781 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 841 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 901 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 961 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC 1021 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 1081 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 1141 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 1201 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 1261 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC 1321 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT 1381 GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAGCT TCAAAAGCGC 1441 TCTGAAGTTC CTATACTTTC TAGAGAATAG GAACTTCGGA ATAGGAACTA AGGAGGATAT 1501 TCATATGGAC CATGGCTAAT TCCCATGTCA GCCGTTAAGT GTTCCTGTGT CACTGAAAAT 1561 TGCTTTGAGA GGCTCTAAGG GCTTCTCAGT GCGTTACATC CCTGGCTTGT TGTCCACAAC 1621 CGTTAAACCT TAAAAGCTTT AAAAGCCTTA TATATTCTTT TTTTTCTTAT AAAACTTAAA 1681 ACCTTAGAGG CTATTTAAGT TGCTGATTTA TATTAATTTT ATTGTTCAAA CATGAGAGCT 1741 TAGTACGTGA AACATGAGAG CTTAGTACGT TAGCCATGAG AGCTTAGTAC GTTAGCCATG 1801 AGGGTTTAGT TCGTTAAACA TGAGAGCTTA GTACGTTAAA CATGAGAGCT TAGTACGTGA 1861 AACATGAGAG CTTAGTACGT ACTATCAACA GGTTGAACTG CGGATCTTGC GGCCGCAAAA 1921 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 1981 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 2041 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 2101 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 2161 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT2221 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 2281 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 2341 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 2401 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 2461 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 2521 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 2581 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 2641 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 2701 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 2761 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 2821 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 2881 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 2941 CGCGCACATT TCCCCGAAAA GTGCCACCTG CATCGATGGC CCCCCGATGG TAGTGTGGGG 3001 TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA 3061 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA 3121 TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG 3181 CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG ATGGCCTTTT 3241 TGCGTGGCCA GTGCCAAGCT TGCATGC//其他大肠杆菌表达载体：pBV221 ptdTomato pET-‐52b(+) pAmCyanpDsRed-‐Express2 pBV220 pCold-‐GST pColdS-‐SUMOpCold T F pCold I V pCold I II pCold I IpCold I pE-‐SUMO pCold-‐ProS2 pBAD102/D-‐TOPOpBAD202/D-‐TOPO pACYC184 pBAD/Thio-‐TOPO pBad/Myc-‐His CpBad/Myc-‐His B pBad/Myc-‐His A pBad/His C pBad/His BpBad/His A pBAD-‐TOPO pET-‐23b(+) pET-‐23a(+)pET-‐23c(+) pET-‐23(+) pET-‐12b(+) pET-‐12c(+)pET-‐12a(+) pET-‐11b(+) pET-‐11a(+) pET-‐11c(+)pBad24 pQE-‐82L pQE-‐81L pQE-‐80LpQE-‐32 pQE-‐9 pQE-‐16 pQE-‐31pQE-‐60 pQE-‐70 pQE-‐40 pET-‐51b(+)pET-‐50b(+) pET-‐49b(+) pET-‐48b(+) pET-‐47b(+)pET-‐26b(+) pET-‐32a(+) pET-‐21b(+) pET-‐22b(+)pET-‐14b pET-‐16b pET-‐15b pET-‐19bpET-‐20b(+) pET-‐21d(+) pET-‐21c(+) pET-‐21b(+)pET-‐21a(+) pET-‐24a(+) pET-‐24d(+) pET-‐25b(+)pET-‐27b(+) pET-‐28a(+) pET-‐30a(+) pET-‐42a(+)pET-‐43.1c(+) pET-‐43.1b(+) pET-‐43.1a(+) pET-‐44a(+)pET-‐44c(+) pET-‐46 E K/LIC pET-‐37b(+) pTrcHis2 CpTrcHis2 B pTrcHis2 A pET303/CT-‐His pET302/NT-‐HispRSET-‐CFP pRSET-‐EmGFP pRSET-‐BFP pGFPuvpET300/NT-‐DEST pET301/CT-‐DEST pGEM-‐T pBad43pGEX-‐4T-‐3 pGEX-‐5X-‐2 pBlueScript S K(+) pG-‐Tf2pG-‐KJE8 pGro7 pET-‐SUMO pSE380pET-‐17b pET102/D-‐TOPO pCDFDuet-‐1 pMAL-‐p5x pTf16 pET-‐28c(+) pBluescript I I S K(+) pET-‐30b(+) pSUMO pProEX H Tc pProEX H Tb pProEX H Ta pKD3 pKD13 pKD46 pTYB1 pTYB2 pTWIN2 pBluescript I I K S(-‐) pTYB12 pMAL-‐p5e pACYCDuet-‐1 pEGM-‐11ZF(+) pEGM-‐7ZF(+) PinPoint X a-‐3 PinPoint X a-‐2 PinPoint X a-‐1 pSP73pSP64 pTWIN1 pTYB11 pTXB1pET-‐5b(+) pBad/gIII C pBad/gIII B pBad/gIII A pET-‐5a(+) pMal-‐p4X pMal-‐p2G pkk223-‐3 pkk232-‐8 pCYB1 pEZZ18 pBAD18 pMAL-‐c5x pMal-‐p2E pMal-‐p2X pET-‐44 E K/LIC pET-‐43.1 E K/LIC pET-‐41 E K/LIC pMal-‐c4X pTrcHis B pET-‐31b(+) pET-‐3b(+) pET-‐41a(+) pGEX-‐3X pGEX-‐4T-‐2 pETDuet-‐1 pGEX-‐4T-‐1 pTrc99a pET-‐28b(+) pET-‐His pALEX a,b,c pACYC177 pBR322 pKD4 pKD20 pMXB10 pEcoli-‐6xHN-‐GFPuv pKJE7 pRSET B pGEX-‐KG pGEX-‐2T pRSFDuet-‐1 pCOLADuet-‐1 pTrcHis C pTrcHis A pET-‐41b(+) pET-‐42b(+) pET-‐3a(+) pGEX-‐6P-‐3 pGEX-‐6P-‐2 pGEX-‐6P-‐1 pGEX-‐5X-‐3 pGEX-‐5X-‐1 pGEX-‐2TK pRSET A pMal-‐c2G pMal-‐c2E pMal-‐c2X pRSET C pQE-‐30pET-‐45b(+) pET-‐44b(+) pET-‐42c(+) pET-‐41c(+) pET-‐40b(+) pET-‐33b(+) pET-‐39b(+) pET-‐32 E K/LIC pET-‐32 X a/LIC pET-‐32c(+) pET-‐32b(+) pET-‐30 X a/LIC pET-‐30 E K/LIC pET-‐30c(+) pET-‐29c(+) pET-‐29b(+) pET-‐29a(+) pET-‐24c(+) pET-‐24b(+) pET-‐24(+) pET-‐23d(+) pET-‐11d(+) pBad33。