pKD3编号 载体名称北京华越洋生物VECT4290 pKD3pKD3载体基本信息载体名称: pKD3质粒类型: 细菌表达；基因敲除系统高拷贝/低拷贝: -‐-‐启动子: R6K克隆方法: 多克隆位点，限制性内切酶载体大小: 2804 b p5' 测序引物及序列: R6K-‐33' 测序引物及序列: rrnB-‐T1-‐term-‐F载体标签: -‐-‐载体抗性: Ampicillin 和 Chloramphenicol筛选标记: -‐-‐备注: -‐-‐稳定性: -‐-‐组成型: -‐-‐病毒/非病毒: -‐-‐pKD3载体质粒图谱和多克隆位点信息pKD3载体序列ORIGIN1 AGATTGCAGC ATTACACGTC TTGAGCGATT GTGTAGGCTG GAGCTGCTTC GAAGTTCCTA 61 TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCAT TTAAATGGCG CGCCTTACGC 121 CCCGCCCTGC CACTCATCGC AGTACTGTTG TATTCATTAA GCATCTGCCG ACATGGAAGC181 CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG 241 TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA 301 AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA 361 ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT 421 GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT 481 GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT 541 TCATTGCCAT ACGTAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG 601 CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT 661 GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC 721 GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT 781 CCTTAGCTCC TGAAAATCTC GACAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT 841 TATGGTGAAA GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC CAAAAGTTGG 901 CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT TTATTCTGCG AAGTGATCTT 961 CCGTCACAGG TAGGCGCGCC GAAGTTCCTA TACTTTCTAG AGAATAGGAA CTTCGGAATA 1021 GGAACTAAGG AGGATATTCA TATGGACCAT GGCTAATTCC CATGTCAGCC GTTAAGTGTT 1081 CCTGTGTCAC TGAAAATTGC TTTGAGAGGC TCTAAGGGCT TCTCAGTGCG TTACATCCCT 1141 GGCTTGTTGT CCACAACCGT TAAACCTTAA AAGCTTTAAA AGCCTTATAT ATTCTTTTTT 1201 TTCTTATAAA ACTTAAAACC TTAGAGGCTA TTTAAGTTGC TGATTTATAT TAATTTTATT 1261 GTTCAAACAT GAGAGCTTAG TACGTGAAAC ATGAGAGCTT AGTACGTTAG CCATGAGAGC 1321 TTAGTACGTT AGCCATGAGG GTTTAGTTCG TTAAACATGA GAGCTTAGTA CGTTAAACAT 1381 GAGAGCTTAG TACGTGAAAC ATGAGAGCTT AGTACGTACT ATCAACAGGT TGAACTGCGG 1441 ATCTTGCGGC CGCAAAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 1501 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 1561 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 1621 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 1681 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 1741 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 1801 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 1861 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 1921 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 1981 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 2041 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 2101 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 2161 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 2221 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 2281 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 2341 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 2401 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 2461 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGCAT CGATGGCCCC 2521 CCGATGGTAG TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA 2581 CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT 2641 CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA 2701 GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC 2761 CTGACGGATG GCCTTTTTGC GTGGCCAGTG CCAAGCTTGC ATGC//其他大肠杆菌表达载体：pBV221 ptdTomato pET-52b(+) pAmCyan pDsRed-Express2 pBV220 pCold-GST pColdS-SUMO pCold TF pCold IV pCold III pCold IIpCold I pE-SUMO pCold-ProS2 pBAD102/D-TOPO pBAD202/D-TOPO pACYC184 pBAD/Thio-TOPO pBad/Myc-His C pBad/Myc-His B pBad/Myc-His A pBad/His C pBad/His B pBad/His A pBAD-TOPO pET-23b(+) pET-23a(+)pET-23c(+) pET-23(+) pET-12b(+) pET-12c(+)pET-12a(+) pET-11b(+) pET-11a(+) pET-11c(+) pBad24 pQE-82L pQE-81L pQE-80LpQE-32 pQE-9 pQE-16 pQE-31pQE-60 pQE-70 pQE-40 pET-51b(+)pET-50b(+) pET-49b(+) pET-48b(+) pET-47b(+)pET-26b(+) pET-32a(+) pET-21b(+) pET-22b(+)pET-14b pET-16b pET-15b pET-19bpET-20b(+) pET-21d(+) pET-21c(+) pET-21b(+)pET-21a(+) pET-24a(+) pET-24d(+) pET-25b(+)pET-27b(+) pET-28a(+) pET-30a(+) pET-42a(+)pET-43.1c(+) pET-43.1b(+) pET-43.1a(+) pET-44a(+)pET-44c(+) pET-46 EK/LIC pET-37b(+) pTrcHis2 C pTrcHis2 B pTrcHis2 A pET303/CT-His pET302/NT-His pRSET-CFP pRSET-EmGFP pRSET-BFP pGFPuvpET300/NT-DEST pET301/CT-DEST pGEM-T pBad43pGEX-4T-3 pGEX-5X-2 pBlueScript SK(+) pG-Tf2pG-KJE8 pGro7 pET-SUMO pSE380pET-17b pET102/D-TOPO pCDFDuet-1 pMAL-p5xpTf16 pET-28c(+) pBluescript II SK(+) pET-30b(+) pSUMO pProEX HTc pProEX HTb pProEX HTa pKD3 pKD13 pKD46 pTYB1pTYB2 pTWIN2 pBluescript II KS(-) pTYB12pMAL-p5e pACYCDuet-1 pEGM-11ZF(+) pEGM-7ZF(+) PinPoint Xa-3 PinPoint Xa-2 PinPoint Xa-1 pSP73pSP64 pTWIN1 pTYB11 pTXB1pET-5b(+) pBad/gIII C pBad/gIII B pBad/gIII A pET-5a(+) pMal-p4X pMal-p2G pkk223-3pkk232-8 pCYB1 pEZZ18 pBAD18pMAL-c5x pMal-p2E pMal-p2X pET-44 EK/LIC pET-43.1 EK/LIC pET-41 EK/LIC pMal-c4X pTrcHis BpET-31b(+) pET-3b(+) pET-41a(+) pGEX-3XpGEX-4T-2 pETDuet-1 pGEX-4T-1 pTrc99apET-28b(+) pET-His pALEX a,b,c pACYC177pBR322 pKD4 pKD20 pMXB10pEcoli-6xHN-GFPuv pKJE7 pRSET B pGEX-KGpGEX-2T pRSFDuet-1 pCOLADuet-1 pTrcHis C pTrcHis A pET-41b(+) pET-42b(+) pET-3a(+) pGEX-6P-3 pGEX-6P-2 pGEX-6P-1 pGEX-5X-3 pGEX-5X-1 pGEX-2TK pRSET A pMal-c2GpMal-c2E pMal-c2X pRSET C pQE-30pET-45b(+) pET-44b(+) pET-42c(+) pET-41c(+) pET-40b(+) pET-33b(+) pET-39b(+) pET-32 EK/LIC pET-32 Xa/LIC pET-32c(+) pET-32b(+) pET-30 Xa/LIC pET-30 EK/LIC pET-30c(+) pET-29c(+) pET-29b(+) pET-29a(+) pET-24c(+) pET-24b(+) pET-24(+)pET-23d(+) pET-11d(+) pBad33。