pET-14b编号 载体名称北京华越洋生物VECT4150 pET-‐14bpET14b载体基本信息别名: pET14b, p et 14b质粒类型: 大肠杆菌蛋白表达表达水平: 高克隆方法: 多克隆位点，限制性内切酶载体大小: 4671bp5' 测序引物: T75' 测序引物序列: 5'-‐TAATACGACTCACTATAGGG-‐3'载体标签: N-‐His, N-‐thrombin载体抗性: Ampicillin备注: Hosts: E.coli. R elated v ectors: p BR322. 稳定性: 瞬时表达 Transient组成型: 组成型 Constitutive病毒/非病毒: 非病毒pET14b载体质粒图谱和多克隆位点信息pET14b载体简介The p ET-‐14b v ector (Cat. N o. 69660-‐3) c arries a n N-‐terminal H is•Tag® s equence f ollowed b y a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that thesequence i s n umbered b y t he p BR322 c onvention, s o t he T7 e xpression r egion i s r eversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA p olymerase i s s hown b elow.pET14b载体序列ORIGIN1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT181 GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC361 CACACCCGTC CTGTGGATAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA421 CCCGTTTAGA GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA GCAGCCAACT481 CAGCTTCCTT TCGGGCTTTG TTAGCAGCCG GATCCTCGAG CATATGGCTG CCGCGCGGCA541 CCAGGCCGCT GCTGTGATGA TGATGATGAT GGCTGCTGCC CATGGTATAT CTCCTTCTTA601 AAGTTAAACA AAATTATTTC TAGAGGGAAA CCGTTGTGGT CTCCCTATAG TGAGTCGTAT661 TAATTTCGCG GGATCGAGAT CTCGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC721 GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG781 GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG841 GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC901 AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT961 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG1021 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG1081 GCAGCGCTCT GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC1141 CTGTCGCTTG CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC1201 GCCACCAAAC GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG1261 GGCTACGTCT TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCAT TATGATTCTT1321 CTCGCTTCCG GCGGCATCGG GATGCCCGCG TTGCAGGCCA TGCTGTCCAG GCAGGTAGAT1381 GACGACCATC AGGGACAGCT TCAAGGATCG CTCGCGGCTC TTACCAGCCT AACTTCGATC1441 ACTGGACCGC TGATCGTCAC GGCGATTTAT GCCGCCTCGG CGAGCACATG GAACGGGTTG1501 GCATGGATTG TAGGCGCCGC CCTATACCTT GTCTGCCTCC CCGCGTTGCG TCGCGGTGCA1561 TGGAGCCGGG CCACCTCGAC CTGAATGGAA GCCGGCGGCA CCTCGCTAAC GGATTCACCA1621 CTCCAAGAAT TGGAGCCAAT CAATTCTTGC GGAGAACTGT GAATGCGCAA ACCAACCCTT1681 GGCAGAACAT ATCCATCGCG TCCGCCATCT CCAGCAGCCG CACGCGGCGC ATCTCGGGCA1741 GCGTTGGGTC CTGGCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG ACCCGGCTAG1801 GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG CGAACGTGAA1861 GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC TTCGGTTTCC1921 GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CAGCGCCCTG CACCATTATG TTCCGGATCT1981 GCATCGCAGG ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA ACGAAGCGCT2041 GGCATTGACC CTGAGTGATT TTTCTCTGGT CCCGCCGCAT CCATACCGCC AGTTGTTTAC 2101 CCTCACAACG TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC GTGAGCATCC 2161 TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC ACGGAGGCAT 2221 CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG CTTTATCAGA AGCCAGACAT 2281 TAACGCTTCT GGAGAAACTC AACGAGCTGG ACGCGGATGA ACAGGCAGAC ATCTGTGAAT 2341 CGCTTCACGA CCACGCTGAT GAGCTTTACC GCAGCTGCCT CGCGCGTTTC GGTGATGACG 2401 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG 2461 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG 2521 CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG CGGCATCAGA 2581 GCAGATTGTA CTGAGAGTGC ACCATATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG 2641 AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 2701 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 2761 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 2821 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 2881 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 2941 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 3001 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 3061 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 3121 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 3181 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 3241 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC 3301 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 3361 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 3421 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 3481 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 3541 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 3601 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC 3661 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC 3721 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA 3781 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC 3841 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 3901 AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA 3961 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA 4021 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA 4081 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT 4141 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 4201 TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG 4261 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA 4321 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC 4381 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG 4441 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 4501 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 4561 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG 4621 ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTTCAAGA A//其他大肠杆菌表达载体：pBV221 ptdTomato pET-52b(+) pAmCyan pDsRed-Express2 pBV220 pCold-GST pColdS-SUMO pCold TF pCold IV pCold III pCold IIpCold I pE-SUMO pCold-ProS2 pBAD102/D-TOPO pBAD202/D-TOPO pACYC184 pBAD/Thio-TOPO pBad/Myc-His C pBad/Myc-His B pBad/Myc-His A pBad/His C pBad/His B pBad/His A pBAD-TOPO pET-23b(+) pET-23a(+)pET-23c(+) pET-23(+) pET-12b(+) pET-12c(+)pET-12a(+) pET-11b(+) pET-11a(+) pET-11c(+) pBad24 pQE-82L pQE-81L pQE-80LpQE-32 pQE-9 pQE-16 pQE-31pQE-60 pQE-70 pQE-40 pET-51b(+)pET-50b(+) pET-49b(+) pET-48b(+) pET-47b(+)pET-26b(+) pET-32a(+) pET-21b(+) pET-22b(+)pET-14b pET-16b pET-15b pET-19bpET-20b(+) pET-21d(+) pET-21c(+) pET-21b(+)pET-21a(+) pET-24a(+) pET-24d(+) pET-25b(+)pET-27b(+) pET-28a(+) pET-30a(+) pET-42a(+)pET-43.1c(+) pET-43.1b(+) pET-43.1a(+) pET-44a(+)pET-44c(+) pET-46 EK/LIC pET-37b(+) pTrcHis2 C pTrcHis2 B pTrcHis2 A pET303/CT-His pET302/NT-His pRSET-CFP pRSET-EmGFP pRSET-BFP pGFPuvpET300/NT-DEST pET301/CT-DEST pGEM-T pBad43pGEX-4T-3 pGEX-5X-2 pBlueScript SK(+) pG-Tf2pG-KJE8 pGro7 pET-SUMO pSE380pET-17b pET102/D-TOPO pCDFDuet-1 pMAL-p5xpTf16 pET-28c(+) pBluescript II SK(+) pET-30b(+) pSUMO pProEX HTc pProEX HTb pProEX HTa pKD3 pKD13 pKD46 pTYB1pTYB2 pTWIN2 pBluescript II KS(-) pTYB12pMAL-p5e pACYCDuet-1 pEGM-11ZF(+) pEGM-7ZF(+) PinPoint Xa-3 PinPoint Xa-2 PinPoint Xa-1 pSP73pSP64 pTWIN1 pTYB11 pTXB1pET-5b(+) pBad/gIII C pBad/gIII B pBad/gIII A pET-5a(+) pMal-p4X pMal-p2G pkk223-3pkk232-8 pCYB1 pEZZ18 pBAD18pMAL-c5x pMal-p2E pMal-p2X pET-44 EK/LIC pET-43.1 EK/LIC pET-41 EK/LIC pMal-c4X pTrcHis BpET-31b(+) pET-3b(+) pET-41a(+) pGEX-3XpGEX-4T-2 pETDuet-1 pGEX-4T-1 pTrc99apET-28b(+) pET-His pALEX a,b,c pACYC177pBR322 pKD4 pKD20 pMXB10pEcoli-6xHN-GFPuv pKJE7 pRSET B pGEX-KGpGEX-2T pRSFDuet-1 pCOLADuet-1 pTrcHis C pTrcHis A pET-41b(+) pET-42b(+) pET-3a(+) pGEX-6P-3 pGEX-6P-2 pGEX-6P-1 pGEX-5X-3 pGEX-5X-1 pGEX-2TK pRSET A pMal-c2GpMal-c2E pMal-c2X pRSET C pQE-30pET-45b(+) pET-44b(+) pET-42c(+) pET-41c(+) pET-40b(+) pET-33b(+) pET-39b(+) pET-32 EK/LIC pET-32 Xa/LIC pET-32c(+) pET-32b(+) pET-30 Xa/LIC pET-30 EK/LIC pET-30c(+) pET-29c(+) pET-29b(+) pET-29a(+) pET-24c(+) pET-24b(+) pET-24(+)pET-23d(+) pET-11d(+) pBad33。