细胞中微量元素含量测定原子吸收光谱法测定细胞中微量元素原子吸收光谱分析基本原理基于从光源辐射出待测元素的特征谱线的光，通过试样蒸气时，被蒸气中待测元素的基态原子所吸收，根据特征谱线的光减弱的程度来测定试样中待测元素含量的方法。
共振线：在原子吸收光谱中能被基态原子吸收的谱线。
2017/11/162017/11/16单光束原子吸收光谱仪示意图高压电源读数器放大器光电倍增管单色器光源原子化器分光系统燃料气载气检测系统试样光源（空心阴极灯）工作原理示意图A = log I0I= k N l光的吸收定律I0 —光源所发射的待测元素“共振线”的强度；A —吸光度；I —被火焰中待测元素吸收后的透光强度；k —原子吸收系数；N —蒸汽中基态原子的浓度；l —“共振线”所通过的火焰长度；A = k C（原子吸收光谱进行定量分析的基本公式）2017/11/16原子吸收光谱仪2017/11/16原子吸收分光光度计2017/11/162017/11/16自动进样器方法特点及应用（1）干扰少准确度高（2）仪器简单操作方便（3）灵敏度高（4）测定元素范围广应用：痕量分析冶金、地质、采矿、石油、化工、环境保护、医药卫生等2017/11/16实例火焰原子吸收光谱法测定淋巴细胞中微量的铜和锌1. 淋巴细胞的分离抽取外用静脉血3.0 ml，以等渗氯化理溶液4׃1稀释后，以Ficoll分离液分离淋巴细胞，以转速2000 rpm，分离30 min。
将分离的淋巴细胞层吸出，加4倍等渗氯化锂后以转速1500 rpm，离心分离10 min，弃去上清液，加1.0 ml 超纯水轻轻振摇1 min，即加高渗氯化锂1.0 ml，振摇1 min，再加等渗氯化锂，清洗3遍，每次离心10 min。
用等渗氯化锂0.12 ml加入经离心沉淀的淋巴细胞中轻轻振摇，使其成为均匀的悬浮液，从中吸取0.02 ml计数，另吸出0.1 ml置于康氏管，加等量胃蛋白酶消化液置于37℃水浴，18 h后，加0.4％硝酸至2.0 ml标定，并按公式换算每个淋巴细胞的铜、锌浓度。
2. 标准加入法曲线制作由于淋巴细胞内铜、锌的含量极低，且灵敏度受到共存物的影响，使用标准加入法可以对基体影响加以校正。
即在等量同一样品中加入标准溶液使铜浓度分别为0、0.1、0.2、0.5、1.0、2.0 mg/ml，绘制标准加入法曲线。
线性方程Cu：Y＝0.021X + 0.001，r＝1.000；Zn：Y＝0.100X + 0.004，r＝0.9992。
3. 精密度按上述方法步骤对同一份淋巴细胞样品进行8次平行试验，结果见表2。
4. 回收率采用样品加标准测定其回收率，来验证方法的准确度。
回收率见表3。
5．应用采用上述方法对克山病区、非病区正常人及潜在、慢性克山病人淋巴细胞内铜、锌进行了调查，结果见下表6。
Pro-inflammatory effects and oxidative stress in lung macrophages and epithelial cellsinduced by ambient particulate matterEnvironmental Pollution. 2013Fig. 1. Illustration of PM-induced intracellular and extracellular toxicological mechanisms in the lung.Fig. 2. Experimental procedure for PM preparation and cell exposure.ICP-MSFig. 3. Cell viability [%] of A549 and RAW264.7 cells during 0e96 h incubation with PM10 samples from urban traffic and rural sites. Each measuring point shows mean standard deviation of one experiment in triplicate. Statistically significant difference between exposed and unexposed cells (*) p < 0.01, (**) p < 0.001, (***) p < 0.0001.Fig. 4. D total GSH content [nmol/106 cells] of A549 and RAW264.7 cells after 0e96 h incubation with urban traffic and rural PM10. The data described as D total GSH content (measured values without control values), below the x-axis the graphic shows the control values. Each bar shows meanstandard deviation of one experiment in triplicate. Statistically significant differences between exposed and unexposed cells (*) p < 0.01, (**) p < 0.001, (***) p < 0.0001.Fig. 5. SOD activity [mmol/min/106 cells] of A549 and RAW264.7 cells after 0-96 h incubation with urban traffic and rural PM10. The data described as D SOD activity (measured values without control values), below the x-axis the graphic shows the control values. Each bar shows mean standard deviation of one experiment in triplicate. Statistically significant differences between exposed and unexposed cells (*) p < 0.01, (**) p < 0.001, (***) p < 0.0001.Fig. 6. CAT activity [nmol/min/106 cells] of A549 and RAW264.7 cells after 0-96 h incubation with urban traffic and rural PM10. The data described as D CAT activity (measured values without control values), below the x-axis the graphic shows the control values. Each bar shows mean standard deviation of one experiment in triplicate. Statistically significant differences between exposed and unexposed cells (*) p < 0.01, (**) p < 0.001, (***) p < 0.0001.Fig. 7. IL-6 release [ng/ml] of A549 and RAW264.7 cells after 0-96 h incubation with urban traffic and rural PM10. The data described as D IL-6 release (measured values without control values), below the x-axis the graphic shows the control values. Each bar shows mean standard deviation of one experiment in triplicate. Statistically significant differences between exposed and unexposed cells (*) p < 0.01, (**) p < 0.001, (***) p < 0.0001.Fig. 7. IL-8 and TNF-ɑrelease [ng/ml] of A549 and RAW264.7 cells after 0-96 h incubation with urban traffic and rural PM10. The data described as D IL-6 release (measured values without control values), below the x-axis the graphic shows the control values. Each bar shows mean standard deviation of one experiment in triplicate. Statistically significant differences between exposed and unexposed cells (*) p < 0.01, (**) p < 0.001, (***) p < 0.0001.。